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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Thrombopoietin TPO Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... microscale transfection was performed (∼1 mL per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2020Quote: ... microscale transfection were performed (~1 ml per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2022Quote: Recombinant human β2m-MR1-K43A-Fc was produced in CHO-K1 cells as previously described (10) and 4 µg/mL were coated onto 96 wells plates (Nunc, Cat#439454) for 18 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... These confluent CHO cells were transfected with 700 ng pUNO1-Mincle or pUNO1-DC-SIGN using TurboFect Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: CHO-suspension cells were plated at a concentration of 7.5x10^4 cells/mL in Freestyle CHO Expression medium supplemented with 8 mM L-glutamine (Thermo Fisher Scientific, MA) in 125-mL disposable polycarbonate Erlenmeyer flasks with vented caps (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (heavy chain to light chain) using ExpiFectamine CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The AF-03 and AF03-NL plasmid were transfected into ExpiCHO-S cells using the ExpiFectamine™ CHO Transfection Kit (Gibco, A29129) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Purified CD4+ T cells were co-cultured with CHO cells for 16 hours in the presence of T cell activation using anti-CD3/CD28 Dynabeads™ (ThermoFisher Scientific) at an estimated 1:1 bead-to-cell ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... microscale transfection was performed (∼1 ml per antibody) with CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected using JetPEI® DNA transfection reagent (CHO dhFr- cells, Polyplus Transfection, Illkirch-Graffenstaden, France) or lipofectamin®2000 reagent (COS-7 and MDCK cells, Invitrogen, Carlsbad, US). Experiments were performed 24h (TIRF imaging ...
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bioRxiv - Immunology 2021Quote: ... the transfected cells were transferred to T-75 flasks at a 2 × 106 vc/mL density for a 1-day recovery in EX-CELL CD CHO Fusion medium (MilliporeSigma, Burlington, MA, USA) with 6mM GlutaMAX (Gibco; Waltham, MA, USA). After 1-day recovery ...
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bioRxiv - Immunology 2021Quote: Sigma Aldrich’s CHOZN cells were cultured in EX-CELL CD CHO Fusion medium (MilliporeSigma, Burlington, MA, USA) supplemented with 6 mM GlutaMAX (Gibco, Waltham, MA, USA), shaking at 37°C ...
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bioRxiv - Biochemistry 2022Quote: For confocal microscopy 25,000 adherent CHO-K1 cells were seeded on 8-well Nunc™ Lab-Tek™ (Thermo Scientific™, United States) and incubated overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The PACRG/pEGFP-N2 and DNALI1/Flag were transfected individually or together into CHO cells by using Lipofectamine™ 2000 transfection reagent (Invitrogen, Waltham, MA). The CHO cells were cultured with DMEM (with 10% fetal bovine serum ...
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bioRxiv - Immunology 2020Quote: ... of Chinese hamster ovary (CHO) cell cultures using the Gibco™ ExpiCHO™ Expression System and a protocol for deep 96-well blocks (ThermoFisher Scientific). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Chinese hamster ovary cells (CHO) (RRID: CVCL_0214) were grown at pH 7.4 in filtered sterile F12 (Ham’s) nutrient medium (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 5% FBS and maintained in a humidified environment at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2020Quote: CHO-K1 and the GAG-deficient variant CHO-745 cells (generous gift from Dr. Antonio Alcami, Spanish Research Council, Spain) were grown in DMEM/F12-Glutamax (Life Technologies, Carlsbad, CA) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... The CHO-K1 cells were grown in low glucose (1.0 g/L) Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, Cat. no. 11,885-084) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: Total RNA extraction and isolation from normal and PSC liver cells or 3D-CHOs were performed using Trizol™ reagent and a PureLink RNA kit (Invitrogen, Waltham, MA). cDNAs were synthesized with a SuperScript™ First Strand Synthesis System (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: Chinese hamster ovary (CHO) cells transfected with CXCR3A were cultured in Ham’s F-12 growth medium (Gibco; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... Flp-In CHO cells were chemically co-transfected with the linearized rDNA and circular pOG44 Flp-recombinase vector using Lipofectamine 3000 (Thermo Fisher Scientific, USA) following their protocol ...
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bioRxiv - Biochemistry 2023Quote: ... of paired heavy and light chain antibody sequences cloned into a single expression vector and expressed in Chinese hamster ovary (CHO) cells using the Gibco™ ExpiCHO™ Expression System and a protocol for deep 96-well plates (ThermoFisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2022Quote: Chinese Hamster Ovary (CHO) cells (ATCC® CCl-61™) were maintained at 37°C and 5% CO2 in DMEM/F-12 (Thermo Fisher, Cat# 11330057) with 10% FBS (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... CHO-K1 cells constitutively expressing human KCNE1 (designated CHO-KCNE1 cells) were generated as previously described (74) using the FLP-in™ system (Thermo Fisher Scientific, Waltham, MA, USA) and maintained under selection with hygromycin B (600 μg/mL).
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bioRxiv - Immunology 2023Quote: ... mouse anti-mouse PCSK9 (Invitrogen), mouse anti-mouse vinculin (Santa Cruz) ...
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bioRxiv - Physiology 2024Quote: ... and TaqMan primers (mouse Panx1 mm0045091, mouse Panx2 mm01308054, mouse Pan×3 mm00552586, mouse Gja1 mm00439105, mouse Gja5 mm00433619– Invitrogen) (Rat Panx1 Rn01447976_m1 – Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: Expression SΔTM trimers was performed by transfection of ExpiCHO-S™ cells with the plasmids described above using the ExpiFectamine™ CHO transfection kit (Gibco™, Thermo Fisher Scientific, Waltham, MA, USA) and according to manufacturer instructions ...
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bioRxiv - Physiology 2021Quote: ... mouse anti-mouse GAPDH (Thermo Fisher, #AM4300), rabbit-anti-mouse LSD1 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... cells were blocked for one hour with mouse-on-mouse blocking reagent (ReadyProbes™ Mouse-on-Mouse IgG Blocking Reagent, 1:30 dilution, ThermoFisher, R37621) between antibody staining procedures.
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-V5 mouse (Invitrogen, # R960-25, 1/1000), mouse anti-FLAG (Sigma ...
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bioRxiv - Immunology 2023Quote: ... PE-Cy5-conjugated mouse anti-mouse CD45.1 (A20; Thermofisher), PE-Cy5 or PE-CF594-conjugated rat anti-mouse CD45R/B220 (RA3-6B2 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Pathology 2024Quote: ... HT7 was the only anti-mouse antibody used and a mouse-on-mouse blocking agent (Invitrogen: R37621) was applied for 30 minutes post protein block washes to reduce noise from secondary antibody binding and an additional three 5-minute washes were added before primary antibody incubation ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IgM-HRP and anti-mouse IgA-HRP (ThermoFisher), goat anti-mouse Igκ ...
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bioRxiv - Plant Biology 2024Quote: Mouse liver microsomes (purchased from Thermo: GIBCO Mouse (CD-1) Microsomes ...
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bioRxiv - Cancer Biology 2022Quote: ... Mm00440280_g1 (mouse, ThermoFisher), and TaqMan™ Universal Master Mix II ...
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bioRxiv - Genomics 2019Quote: ... α-mouse (Invitrogen #A31570), α-goat (Invitrogen #A21432) ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse laminin (Gibco) was then added and incubated at 37°C for 4 hours ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... mouse (Affymetrix, 902931), performed by Cnkingbio Biotech Co. ...
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bioRxiv - Cell Biology 2021Quote: ... GST (Mouse, Invitrogen), GM130 (Mouse ...
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bioRxiv - Cell Biology 2023Quote: ... mouse (ThermoFisher, #23017015); Vitronectin ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse (Invitrogen, A315570), and rabbit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ZO-1 (mouse monoclonal, Invitrogen 33-9100, 1:500), rabbit anti-aPKC (rabbit polyclonal ...
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bioRxiv - Immunology 2020Quote: ... mouse IFNβ (PBL Biomedical Laboratories) and mouse CXCL10 ELISA (ThermoFisher; BMS6018MST) were obtained from commercial sources.
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bioRxiv - Developmental Biology 2023Quote: ... Mouse aggregation medium (mouse AM) consisted of 1x DMEM (GIBCO-41965) supplemented with 20% FBS (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-©tubulin (mouse monoclonal, MilliporeSigma T6557, 1:200) and mouse anti-ZO-1 (Life Technologies, 1:300). Rabbit anti-Pard3 (rabbit polyclonal ...
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bioRxiv - Physiology 2020Quote: ... βTC3 cells were transfected with plasmids encoding mouse furin and/or mouse insulin receptor (pcDNA3.1 backbone) and/or mouse Atp6ap1/Ac45-Flag using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... for more than 1.5 hours in a shaker and incubated with the primary antibody (Mouse anti- GAD67, Millipore; Mouse anti-CaMK2a, Abcam; Mouse anti-mCherry, Invitrogen) in 0.2% Triton and 5% Goat serum in PBS at 4 °C for 24 -36 hours ...