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Citations for Thermo Fisher :
451 - 500 of 7949 citations for Tau Methyl L Histidine Methyl D3 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... pET151/D-TOPO plasmids with the sequences of interest inserted under control of Lac operon and T7 viral promoter with N-terminal hexa-histidine tag and Ampicillin selection marker was obtained from Invitrogen. Sequences were optimized for expression in E ...
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bioRxiv - Cell Biology 2022Quote: Histidine-tagged Mindin was purified from conditioned media collected from CHO-Mindin cells using Ni-NTA beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: The T7 gp5.9 gene from phage T7 was produced as a synthetic gene construct with an N-terminal 3C-cleavable histidine tag (GeneArt, Invitrogen). This was subcloned into the pACEBAC1 (MultiBac ...
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Microbiology 2024Quote: ... They were produced with a histidine-tag in the Bac-to-Bac™ Baculovirus Expression System (Invitrogen, for VP3 proteins) or Escherichia coli prokaryotic system (for 2xFYVE) ...
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bioRxiv - Cell Biology 2019Quote: Different lengths of the promoter regions of both CAP-D2 and CAP-D3 were cloned between the SalI and NotI restriction sites of the pEntr 1A plasmid (Invitrogen). The sequences were amplified from gDNA with the primer pairs D2-1156F/D2ProR for the Pro4 fragment ...
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bioRxiv - Neuroscience 2019Quote: ... The hCMEC/D3 96-well plates had been pre-coated with 0.1mg/ml rat tail collagen type1 (Gibco cat#A1048301).
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bioRxiv - Cell Biology 2022Quote: ... Both the LentiCRISPRv2-sgRNA plasmid and the ss donor oligonucleotides were transfected into D3 mouse ESC line using Lipofectamine 3000 (Invitrogen). Transfected cells were selected with puromycin (1.5 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Both the LentiCRISPRv2-sgRNA plasmid and the ss donor oligonucleotides were transfected into D3 mouse ES cell line using lipofectamine 3000 (Invitrogen). Transfected cells were selected with puromycin (1.5 μg/ml ...
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bioRxiv - Physiology 2023Quote: Cells from the human brain microvascular EC line hCMEC/D3 [37] were seeded on collagen I-coated 35 mm tissue culture dishes (Nunc) and grown in EGM2-MV (Lonza).
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bioRxiv - Cell Biology 2021Quote: ... 2 mmol/L L-glutamine (Invitrogen), 4×104 monothioglycerol (MTG ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM/L L-glutamine (Invitrogen), 4×104 monothioglycerol (MTG ...
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bioRxiv - Immunology 2022Quote: ... 2 mmol/L l-glutamine (Gibco), N2- and B27-supplement (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... 1% L-glutamine (L-glu, Gibco), 1% sodium bicarbonate (NaHCO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mmol/L L-glutamine (Gibco), and 100 mg/mL streptomycin/100 U/mL penicillin (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mmol/L L-glutamine (Gibco), 0.1 mmol/L beta-mercaptoethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 % L-Glutamine (L-glut, Gibco) and 1 % Penicillin/ Streptomycin (P/S ...
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bioRxiv - Neuroscience 2021Quote: ... we used the following mouse monoclonal antibodies: anti-tau AT8 (pS202/pT205; 1:1000; Thermo Scientific), anti-phospho-TDP-43 (pS409/410 ...
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bioRxiv - Neuroscience 2020Quote: ... primary antibodies were added (mouse anti-phospho-Tau [Ser202, Thr205] monoclonal antibody [AT8], 1:150, ThermoFisher cat#MN1020 ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal antihuman phospho-Tau (S202, T205) AT8 (1:1000, Thermo Fisher Scientific, MN1020, UK) for dystrophic neurites ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting tau fibrils were fluorescently labeled with AlexaFluor 647 NHS-ester dye (Invitrogen, Carlsband, CA) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... followed by desalting and buffer exchange into tau buffer over Zeba protein desalting columns (Thermo Fisher).
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bioRxiv - Biochemistry 2023Quote: ... an aliquot (30 μL) of resuspended beads and anti-Tau-5 antibody (Thermo Fisher, 1:250) were added to prepared lysates and incubated at 4 °C overnight with rotation ...
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bioRxiv - Neuroscience 2023Quote: ELISA was performed on total and SEC fractions using Tau (Total) Human ELISA Kit (KHB0041, Invitrogen) by following the directions provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant tau was labeled using an Alexa Fluor 647 Protein Labeling Kit (Cat No. A20173, Invitrogen) with 500 mg of recombinant protein input at a concentration of 1 mg/mL according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with primary antibody (mouse anti-phospho-Tau (AT8, 1:500, Invitrogen, UK), rabbit anti-NeuN (D3S3I ...
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bioRxiv - Biochemistry 2023Quote: ... Concentrations of purified tau protein were determined using a reducing agent compatible BCA assay (Thermo Fisher). Protein purity of final preparations was assessed by coomassie-stained SDS-PAGE or MALDI-TOF Mass spectrometry (Bruker Autoflex Speed MALDI-ToF ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections were incubated in primary antibodies for P-Tau (AT8) (Invitrogen, Grand Island, NY, USA) Immunoreactivity was labeled using appropriate fluorescent secondary antibodies conjugated to Alexa-fluor 568 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated from 25-98°C in a QuantStudio Flex 12 RT-PCR machine (ThermoFisher Scientific). Measurements of fluorescence emission at 570nm were followed for each 0.3°C increment ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-induced (98°C) antigen retrieval was performed with a citrate buffer (pH 6.0) (Thermo Fisher Scientific), and slides were incubated with Triton X-100 0.25% in PBS for 5 minutes ...
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bioRxiv - Biophysics 2020Quote: ... and unprocessed H57-scFV (still containing a 12x histidine tag) were removed by a batch purification with Ni-NTA agarose (ThermoFischer Scientific) and glutathione agarose (ThermoFisher Scientific). The supernatant containing biotinylated H57-scFV was further purified via gel filtration using Superdex 75 (10/300 GL ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant proteins containing a hexa-histidine tag was purified by passing through the nickel-NTA-affinity Column (HisPurTM Ni-NTA Spin Columns, Thermo Fisher) following supplier’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with BsiWI and ligated in frame with a N-terminal 3 x FLAG-tag and a C-terminal 6 × histidine tag into a modified and BsiWI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH1.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Both of the in vitro systems depend on the application of three-dimensional embryoid body cultures derived from either mouse embryonic stem cells (mESC, ES-D3, ATCC, CRL-1934) or human episomal induced pluripotent stem cells (hiPSC, Gibco, A18945). The teratogenicity determination of the mEST is based on the half maximal inhibition of differentiation into beating cardiomyocytes (ID50 ...
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bioRxiv - Neuroscience 2021Quote: ... or with a pool of two different USP30 siRNAs (D1: 5’-CAAAUUACCTGCCGCACAA-3’; D3, 5’-ACAGGAUGCUCACGAAUUA-3’, Dharmacon; siUSP30) by using Lipofectamine RNAiMAX (Thermo Scientific) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Clathrin and caveolin-1 knockdown in polarized hCMEC/D3 cell monolayers was performed using siRNA kit containing RNAi mix (Invitrogen, CA) and reduced serum medium ...
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bioRxiv - Neuroscience 2022Quote: ... GFP and non-GFP K44-negative-dominant dynamin transfections of hCMEC/D3 cell monolayers were performed using Lipofectamine reagent (Invitrogen, CA) as per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The nuclear pellet was washed with 1X wash buffer C and then once with shearing buffer D3 with Halt protease inhibitor cocktail (Thermo scientific)] ...
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bioRxiv - Cell Biology 2022Quote: All cells were cultured and imaged in high-glucose DMEM (4.5 g/L D-glucose, 0.584 g/L L-glutamate, 110 mg/L sodium pyruvate; Gibco) supplemented with 10% fetal bovine serum (MedSupply Partners) ...
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bioRxiv - Microbiology 2023Quote: ... 4 g/L dextrose, 5.957 g/L HEPES, 0.05 g/L hypoxanthine, 5 g/L Albumax II, Invitrogen) supplemented with 200 mM L-glutamine ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM L-glutamine (L-glut, Invitrogen) and either 400 μg/mL Neomycin (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... 2 mM L-alanine-L-glutamine (Gibco), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... 2mM L-alanyl-L-glutamine dipeptide (Gibco) and in growth medium additionally FGF (20ug/mL ...
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bioRxiv - Immunology 2022Quote: ... with 2mM L-Glutamine (L-Glu: Thermofisher), 10% FBS and 1% P/S ...
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bioRxiv - Immunology 2022Quote: ... 2 mmol/L L-glutamine (Thermo Fisher) and 50 U/mL/50 ug/mL of Penicillin/Streptomycin (Thermo Fisher ...
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bioRxiv - Bioengineering 2023Quote: ... 30 mL/L L-glutamine (Gibco, 25030081) and 0.5 mL/L geneticin (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 mmol/L L-glutamine (Gibco). The cells were passaged every 2-3 days and passages 3-7 were used in the current study.
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bioRxiv - Cancer Biology 2023Quote: ... and 2 mmol/L L-glutamine (GIBCO). HEK293T cells (CRL-11268 ...
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bioRxiv - Molecular Biology 2023Quote: ... lectin (L-21409 or L-32459, ThermoFisher) was diluted at 1/500 in 1X PBS and incubated for 1 h at room temperature along with secondary antibodies (see (Comptour et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4.5 g/L glucose (1g/L, Gibco #31600-091 ...