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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for TREM 1 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... FreeStyle™ 293-F cells were grown in FreeStyle™ 293 Expression Medium (Gibco) under constant agitation at 37 °C with 5% CO2 in a humidified incubator.
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bioRxiv - Biochemistry 2021Quote: ... FreeStyle 293-F cells were grown in suspension in FreeStyle 293 Expression Medium (Gibco).
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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A quantitative analysis of the interplay of environment, neighborhood and cell state in 3D spheroidsbioRxiv - Systems Biology 2020Quote: T-REx-293 cells (Invitrogen) and DLD1 cells (Flp-In T-Rex DLD-1 ...
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bioRxiv - Microbiology 2021Quote: ... T-REx-293 cells (Invitrogen) were grown in DMEM supplemented with blasticidin (10 µg/mL ...
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bioRxiv - Microbiology 2021Quote: T-REx-293 cells (Invitrogen), which constitutively expresses the Tet repressor (TetR ...
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bioRxiv - Microbiology 2021Quote: FreeStyle 293-F (Thermo Fisher) cells were grown to a density of 1×106 cells/mL at 37°C with 8% CO2 with regular 135 rpm agitation ...
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bioRxiv - Systems Biology 2022Quote: ... ExpiFectamine 293 Reagent (Thermo Fisher) was diluted with Opti-MEM separately then combined with diluted plasmid DNA for 10 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... Flp-In 293 cells (Invitrogen) were cultured in DMEM media supplemented with fetal bovine serum (10% ...
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bioRxiv - Cancer Biology 2020Quote: ... GSE6532 ((n= 293, platform: Affymetrix Human Genome U133A and B Array ...
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bioRxiv - Molecular Biology 2022Quote: T-Rex-293 cells (Invitrogen) were cultured and maintained as previously described63 in DMEM (Gibco 11-965-092 ...
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bioRxiv - Physiology 2022Quote: ... ExpiFectamine 293 Reagent (Thermo Fisher) was diluted with Opti-MEM separately then combined with diluted plasmid DNA for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... FreeStyle 293-F (Thermo Fisher) cells were transiently co-transfected using PEI MAX (Polysciences ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmid DNA (1 µg/ml culture) and ExpiFectamine™ 293 Reagent (Gibco) were diluted in Opti-MEM® I medium (Gibco ...
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bioRxiv - Immunology 2020Quote: Recombinant HIV-1 Env gp120 was expressed in Freestyle 293 cells (ThermoFisher) by transient transfection ...
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bioRxiv - Biochemistry 2023Quote: ... in 1 ml of Freestyle™ 293 Expression Medium (ThermoFisher Scientific, 12338026) supplemented with 2% heat inactivated FBS (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Equal amounts of heavy and light chain expression plasmids and a 1:3 ratio of PEI was used to transfect 293-6E cells at a density of 1×10^6 cells/mL in Freestyle 293 media (Thermo Fisher Scientific, Cat#: 12338018). Supernatants were collected 6 days post transfection by centrifugation at 4,000g followed by filtration through a 0.22 μM filter (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... ATCC CRL-1586) and HEK-293T (human embryonic kidney cells) cells were maintained in Dulbecco’s Modified Eagle Medium (Gibco). All media were supplemented with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Immunology 2020Quote: ... human embryonic kidney cells (HEK) 293T were grown in a T75 flask with Dulbecco’s modified eagle medium (DMEM) (Gibco) + 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... human embryonic kidney 293T cells (HEK; CRL-3216; ATCC; RRID: CVCL_0063) were cultured in DMEM media (12430062; Gibco™) with 10% FBS (098-150 ...
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bioRxiv - Microbiology 2022Quote: Human HEK-293T adherent cells were seeded at 37°C with 5% CO2 in DMEM + GlutaMAX™ (Gibco, Germany) containing 10% heat inactivated fetal calf serum (dFCS ...
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bioRxiv - Microbiology 2023Quote: Human telomerase immortalized fibroblasts (MRC5-hT) and HEK 293T cells were cultured in Dulbecco’s Modified Eagle Media (DMEM; Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... and Gentamycin 40µg/mL and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... or an untransfected wild type HEK Flp-in cell line (HEK wt, Invitrogen, R75007) were cultured in high-glucose DMEM (HyClone ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293Trex ERGIC53 KO or HEK 293Trex SURF4 KO cells using lipofectamine 2000 (Invitrogen) or polyethyleneimine (PEI) ...
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bioRxiv - Immunology 2022Quote: ... HEK 293F cells were maintained in HEK 293F in 293FreeStyle expression medium (Life Technologies) and cultured at 37°C with 8% CO2 while shaking at 125 rpm ...
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bioRxiv - Neuroscience 2020Quote: FreeStyle 293-F cells grown in serum-free FreeStyle 293 Expression Medium (Gibco, 12338-018) were transfected with Sez6L2MH or His-DAF plasmids using Fectopro (Polyplus ...
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bioRxiv - Microbiology 2019Quote: ... 293 F cells were thawed and incubated with FreeStyleTM 293 Expression Medium (Life Technologies, CA) in a shaker incubator at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) were coupled with 200 µg of histidine-tagged SARS-CoV-2 S protein ...
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bioRxiv - Synthetic Biology 2021Quote: ... HEK-293FT cells (Thermo Fisher), and HeLa cells (ATCC ...
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bioRxiv - Synthetic Biology 2020Quote: ... HEK-293FT cells (Thermo Fisher), HeLa cells (ATCC) ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293T cells (Life Technologies) were maintained in Dulbecco’s modified Eagles medium (DMEM ...
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bioRxiv - Biochemistry 2022Quote: HEK Viral Production cells (ThermoFisher) were maintained in suspension in Freestyle F17 (ThermoFisher ...
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bioRxiv - Physiology 2023Quote: HeLa and HEK 293T (Invitrogen) cells were grown in high glucose Dulbecco’s Modified Eagle Medium with high glucose ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: ... Expi293 HEK cells (Thermofisher, #A14527) were expanded and passaged in Expi293 expression media (Thermofisher ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-293T cells (Life Technologies) were cultured in DMEM High Glucose (4.5 g/L ...
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bioRxiv - Biophysics 2023Quote: ... and HEK 293A (Thermo Fisher) cells were cultured at 37 °C in a 5% CO2(g ...
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bioRxiv - Cancer Biology 2024Quote: The HEK 293FT (Invitrogen, # R70007) cell line was transfected with either pBabe-puro-HA-KRASG12C (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: HEK Viral Production cells (ThermoFisher) were maintained in suspension in Freestyle F17 (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: The knock-out cell line (GTPBP5KO) was generated in the Flp-In T-Rex human embryonic kidney 293 (HEK293T) cell line (Invitrogen) using CRISPR/Cas9 technology targeted on exon 1 of MTG2 gene ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney 393T cells were adapted to suspension culture in Gibco Freestyle 293 medium (Thermo Fisher Scientific, Waltham USA) supplemented with 20 U/L penicillin ...