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Citations for Thermo Fisher :
601 - 650 of 7360 citations for TNFRSF10C Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: FreeStyleTM 293-F cells were purchased from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2 using FreeStyle 293 Expression Medium (ThermoFisher). Cells were maintained in suspension format using disposable shaker flasks placed on Innova 2100 platforms (Eppendorf ...
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bioRxiv - Biochemistry 2022Quote: ... using the ExpiFectamine 293 Transfection Kit (Thermo Fisher) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... the Flp-In T-REx 293 system (Invitrogen) was used as described.17 Protein extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... Flp-In T-Rex 293 (obtained from ThermoFisher) and HeLa Flp-In T-Rex (authenticated with Eurofins ...
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bioRxiv - Neuroscience 2023Quote: Flp-In-293 cells (ThermoFisher Scientific Ref R75007) were transfected according to standard lipofection procedure (Lipofectamine 2000 ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: FreeStyle™ 293-F cells (Thermo Fisher R79007) were separately transfected using PEI MAX® - linear polyethylenimine hydrochloride (MW 40,000 ...
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bioRxiv - Biochemistry 2023Quote: ... in Freestyle 293 expression medium (Thermo Fisher Scientific), cells were transduced with baculovirus and supplemented with 5 mM sodium butyrate ...
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bioRxiv - Molecular Biology 2023Quote: ... 293-FT cells were obtained from Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: Flp-In T-REx 293 cells (Life Technologies) were maintained in 10% FBS and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Microbiology 2022Quote: The plasmids pVRC containing human codon-optimized RBD constructs were transiently transfected into Expi293F cells using the ExpiFectamine™ 293 Transfection Kit (Thermo Fisher, Waltham, MA). Briefly ...
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bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the mixture incubated for 5 min before addition to the shaker flask containing 106 Freestyle 293-F cells/mL grown in suspension in Freestyle 293 expression media (Thermo Fisher). Expression was allowed to continue for 6 days before purification.
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bioRxiv - Immunology 2023Quote: ... and B2(TN99-419) antigens were expressed in FreeStyle 293-F cells by transient transfection in FreeStyle 293 expression media (Thermo Fisher). Cells were co-transfected at a 4:1 ratio of plasmids encoding human metapneumovirus F and furin ...
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bioRxiv - Biochemistry 2022Quote: All recombinant human huntingtin and PRKACA cDNA plasmids were transfected into HEK 293T cells or HTT null HEK 293T cells with Lipofectamine 3000 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... For neutralization assays HEK 293T expressing the SARS-CoV-2 receptor ACE2 (HEK 293T/ACE2) were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Adherent PB2-expressing HEK-293T (HEK-293T-PB2) cells (41) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, #41966029) supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK-Lucia RIGI and HEK-Dual TLR3 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies, Waltham, MA) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Biophysics 2021Quote: ... of Plasmodium falciparum plasmepsin X (XP_001349441) (Fig. 5a) was engineered for expression in a mammalian host (Human Embryonic Kidney cells, HEK cells) by GeneArt (Thermo Fisher, Waltham, MA, USA) and cloned into a variant of pTT5 with the mouse Ig secretion signal peptide on the N terminus and a TEV-cleavable 8x Histidine tag on the C terminus ...
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bioRxiv - Neuroscience 2023Quote: Plasma membrane (PM) fraction was prepared from mouse or human DRG tissues and HEK cells using Mem-PER Plus Kit (Thermo Scientific, Cat No. 89842) and manufacture recommend protocol after biotinylation/streptavidin-magnetic bead (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: HEK-293T cells were cultured in DMEM (Invitrogen) supplemented with 10% heat inactivated FBS (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... HEK-GCaMP6s cells were grown in DMEM:F12 (Invitrogen) containing 5% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK-293T cells were cultured in DMEM (Gibco), supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: HEK-293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK 293FT cells were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: HEK-Blue hNOD1 cells (Invitrogen, hkb-hnod1, USA) were grown in DMEM containing 4.5 g/l glucose ...
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bioRxiv - Biophysics 2022Quote: ... into HEK 293T cells with Lipofectamine 2000 (Gibco). Media was harvested after 48 hours ...
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bioRxiv - Biochemistry 2022Quote: HEK-293A cells (R70507, Life Technologies, Carlsbad, CA) and HEK-293T cells (ATCC ...
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bioRxiv - Genomics 2022Quote: ... HEK 293T was cultured in DMEM (Life Technologies) with 10% FBS (Peak Serum) ...
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bioRxiv - Neuroscience 2019Quote: ... HEK cells were grown in DMEM (GIBCO 11995) containing 10% FBS (Thermo Fisher Scientific A3840101) ...
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bioRxiv - Microbiology 2019Quote: HEK cells were grown in a DMEM (Gibco) containing 10% (vol/vol ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... HEK 293FT cells were obtained from Thermo Scientific. K562 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) ...
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bioRxiv - Cell Biology 2021Quote: HEK 293FT cells were purchased from Thermo Fisher Scientific (cat# R70007 ...
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bioRxiv - Cancer Biology 2022Quote: HEK-293T (ATCC) was grown in DMEM (Gibco) supplemented with 10% FCS (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... HEK 293T (ATCC) were cultured in DMEM (Invitrogen) with 10% FBS and 1% penicillin/streptomycin antibiotics ...
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Long-term precision editing of neural circuits in mammals using engineered gap junction hemichannelsbioRxiv - Neuroscience 2023Quote: HEK 293FT cells were purchased from Thermo Fisher Scientific (cat# R70007 ...
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bioRxiv - Genetics 2023Quote: The HEK-293FT cells (Thermo Fisher Scientific, R70007) were cultured to be 70-90% confluent at the point of transfection to produce lentiviruses ...
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bioRxiv - Cell Biology 2023Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 1% PS ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T cells were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum and 1% penicillin- streptomycin (Gibco).
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bioRxiv - Biochemistry 2024Quote: ... HEK-293F cells were purchased from Thermo Fisher Scientific company ...