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Citations for Thermo Fisher :
101 - 150 of 6133 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... Cells were grown to 80% confluency at 26 °C in T175 tissue culture flasks containing 25 ml RPMI 1640 media (Life Technologies) supplemented with 10% foetal bovine serum (Life Technologies) ...
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bioRxiv - Zoology 2020Quote: ... Tissues were cut into small pieces with a scalpel and washed twice in ADPBS containing antibiotics prior to placing 4-6 tissue pieces into a 25 cm2 plug-seal tissue culture treated flask (BioLite, Thermo Scientific) containing 1.5 mL establishment medium to cover the tissue pieces ...
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bioRxiv - Cell Biology 2021Quote: ... Subsequently the pulp cells were plated in a flask with 25 cm2 growth area containing 5 ml of Iscove’s Modified Dulbecco’s medium (IMDM) (Gibco™, Carlsbad, USA) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: Inoculated starter cultures of 6mL LB-kanamycin that were shaker incubated at 37C 250rpm overnight were used to make 25% glycerol stocks and inoculate in a 1:50 ratio TB-kanamycin in baffled 1L flasks (Thermo Fisher). Cultures were shaker incubated at 37C 250rpm until OD600 (Nanodrop ...
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bioRxiv - Neuroscience 2022Quote: ... Mouse NSPCs were cultured in a PDL/Laminin coated 25 cm2 flask at 10,000 cells per cm2 concentration using Neurobasal plus medium (GIBCO, Life Technologies), supplemented with 2% B27 (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... Green monkey kidney fibroblast cells (Vero) were grown in 25 cm2 flasks with MEM media supplemented with 10% fetal serum (Gibco, USA) and incubated at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Immunology 2022Quote: ... BM cells were then placed into culture in T-75 flasks with BMDM media (DMEM base, 10% FBS, 1x Anti Anti (Gibco, 15240-062), 1x MEM-NEAA (Gibco ...
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bioRxiv - Microbiology 2021Quote: 293T cells plated 24 hours previously at 2 × 107 cells per T-175 flask were transfected using Lipofectamine 2000 (Invitrogen, #11668-019) using manufacturers protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and HT-22 cells (undefined-sex mouse hippocampal cell line) were grown in t-75 flasks containing Dulbecco’s Modified Eagle Medium (DMEM) (GIBCO; Gaithersburg, MD, USA) with added 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: VeroE6 cells were seeded at 106 cells per flask in T25 flasks (Nunc) and the following day infected at MOI 0.000016 with SARS-CoV-2/human/Denmark/DK-AHH1/2020 ...
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bioRxiv - Neuroscience 2021Quote: ... Neural precursors were manually picked and transferred to 25 cm2 flasks in NIM medium supplemented with B27 and ascorbic acid (NIM + 2% B27 supplement (Gibco, 17504-044), 0.1% ascorbic acid (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... MDA-MB-231 human breast adenocarcinoma cells were maintained in adherent culture on T-75 tissue culture flasks in Dubelco’s modified eagle medium (Gibco from Thermo Fisher Scientific) supplemented with 10% FBS (Atlas Biologicals ...
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bioRxiv - Neuroscience 2023Quote: ... in T75 flasks (ThermoFisher, 156499) in 5% CO2 at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... T175 flask (Thermofisher, Massachusetts, USA). Mouse pre-adipocyte 3T3-L1 cells (Catalogue no ...
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bioRxiv - Neuroscience 2024Quote: ... in cell culture flasks (Nunc, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: MAb IgG proteins in supernatants of cloned hybridoma cell lines were prepared by washing cells from T-75 flasks in serum-free Hybridoma-SFM Medium (Thermo Fisher Scientific, cat. # 12045076) and seeding 3 to 6 wells of a 6-well G-Rex plate (Wilson Wolf ...
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bioRxiv - Neuroscience 2024Quote: ... dispersed and cells were cultured on T-75 cell culture flasks in Dulbecco′s Modified Eagle′s Medium (DMEM; Thermo Fisher Scientific, Waltham, UK) substituted with 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2019Quote: ... MSCs were expanded in 5 mL of expansion medium in Nunc™ EasYFlask™ 25 cm2 (T25) cell culture flasks (Thermo Fischer Scientific Nunc A/S). In both cases ...
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bioRxiv - Microbiology 2019Quote: ... coated flasks in neurobasal media (Invitrogen) containing Neural Survival Factor (Lonza) ...
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bioRxiv - Cancer Biology 2021Quote: ... in non-treated culture flasks (Nunc). U87 ...
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bioRxiv - Cell Biology 2023Quote: ... culture flasks with DMEM-F12 (Gibco) supplemented with 20 µg/mL endothelial cell growth supplement (Gibco ...
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bioRxiv - Pathology 2024Quote: ... in a T25 culture flask (Nunc) and incubated at 37°C and 5% CO2 ...
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bioRxiv - Genomics 2019Quote: ... Activation was performed by addition of Dynabeads Human T-Activator CD3/CD28 (25 ul/0.5 million cells; Life Technologies), along with IL-2 (30 U/ml ...
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bioRxiv - Evolutionary Biology 2021Quote: ... which consisted of two baffled shake flasks (250 mL baffled Erlenmeyer Flask, Thermo Fisher Scientific, 4116-0250) connected in series (Fig ...
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bioRxiv - Bioengineering 2023Quote: ... and 106 MSCs were seeded into an initial T-150 tissue culture flask in complete media containing Gibco™ Minimum Essential Media α with nucleosides (Thermo Fisher Scientific, Waltham, MA), 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting single cell suspension was transferred into T-75 flasks in ∼10 ml of neural induction media (50% DMEM/F12 (Gibco/Thermo Fisher Scientific, Cat. No. 11320-033) and 50% Neurobasal-A (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: One million cells PBMCs were incubated in 24 well plates with 30 IU/mL IL-2 (NCI) and with or without 25 μL of Human T-Activator CD3/CD28 dynabeads (ThermoFisher). Following 48 hours ...
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bioRxiv - Immunology 2023Quote: ... cells were washed and re-cultured with Dynabeads mouse CD3/CD28 T cell stimulator (25 μl/ml) (Thermo Fisher Scientific) and IL-2 (1500 U/ml ...
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bioRxiv - Bioengineering 2024Quote: ... CAR-T cells were counted and plated with GFP/Luc tumoroids at the stated E:T ratios in half ‘neurosphere medium’ (expanded on in the Cell Lines section of Methods and Materials) and half T cell medium (RPMI-GlutaMAX 1640 + 25 mM Hepes (ThermoFisher) supplemented with 10% FCS (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1h at room temperature with blocking buffer (3% skim milk powder dissolved in tris-buffered saline supplemented with 0.1% Tween-20 [TBS-T]) and then probed with CV3-25 mAb (1ug/mL) followed by HRP-conjugated anti-human IgG (1:3000, Invitrogen). HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences ...
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bioRxiv - Evolutionary Biology 2021Quote: ... in standard T75 flasks (Thermo Fisher Scientific). Media was changed every three days ...
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bioRxiv - Bioengineering 2020Quote: ... were expanded in T75 flasks (Fisher Scientific) and cultured in endothelial cell growth media (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... thirty 75cm2 cell culture flasks (Nunc, USA), containing 7×106 Acanthamoeba castellanii cells/flask ...
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bioRxiv - Microbiology 2022Quote: T75 flask (Thermo Fisher Scientific, catalog #: 156499).
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bioRxiv - Microbiology 2022Quote: T225 flask (Thermo Fisher Scientific, catalog #: 159934).
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bioRxiv - Molecular Biology 2019Quote: ... were plated in T25 flasks and transiently transfected with 2.5 µg plasmid DNA per flask using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Activation of CD4+ T cells was achieved by addition of 25 U/ml of interleukin-2 (IL-2) and CD4+ T-cell activation Dynabeads (Life Technologies). CD4+ T-cells activated for 4-5 days were used for HIV-1 infections ...
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bioRxiv - Immunology 2020Quote: ... Activated CD8+ T cells were maintained in DMEM medium (10% FCS) with 25 mM (HG) or 5.6 mM (NG) glucose (Thermo Fisher Scientific). Raji cells were maintained in RPMI 1640 medium (10% FCS ...
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bioRxiv - Genomics 2021Quote: ... poly(A)+ RNA was extracted using Oligo d(T)25 Magnetic Beads using the manufacturer’s recommended protocol (Thermo Fisher Scientific, 61002). Poly(A)+ concentration was measured using Qubit™ RNA HS Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... which is composed of two variable bases at its end followed by the poly(T)25 and then with a random adapter sequence using the Superscript III Reverse Transcriptase (Invitrogen, USA). The taqman based q-RT-PCR was performed using the TaqMan Fast Universal PCR Master Mix (2X ...
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bioRxiv - Microbiology 2021Quote: ... small_pMA-T and large_pMA-T (synthesized by Invitrogen). To construct the final plasmid ...
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bioRxiv - Physiology 2021Quote: ... were incubated in a membrane cell culture flask following the manufacturer’s instructions (Wheaton CELLine Bioreactor Flask and Hybridoma-SFM ThermoFisher 12045076). Crosslinking of Y-3 antibody and Protein A+G was done using sulfosuccinimidyl (4-iodoacetyl)aminobenzoate (Sulfo-SIAB ...
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bioRxiv - Neuroscience 2020Quote: ... The mixture of dissociated cells was plated in a T75 culture flask (Nunc EasyFlask 75cm2 cell culture flasks, Thermo Scientific), previously coated with poly-D-lysine hydrobromide (PDL ...
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bioRxiv - Cell Biology 2022Quote: ... Monocytes were seeded in T75 cell culture flasks (5 x 106 cells/flask) in α-minimal essential medium (αMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... 15140-122]) in T150 flasks (Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... Nunc non-treated T175 flasks (Thermo Scientific 159926) were coated with 9ug/mL retronectin (Takara bio T110B ...
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bioRxiv - Neuroscience 2021Quote: ... Flasks were passaged when confluent using TrypLE (Gibco) and plated at a density of 100,000 cells per 13mm coverslip.
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bioRxiv - Genetics 2021Quote: ... Cells were grown in T225 flasks (Thermo Fisher) and regularly passaged at 80-90% confluence with 10x concentrated Tryple Express (Thermo Fisher) ...
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bioRxiv - Physiology 2020Quote: ... T25 flasks (Nunc™, Thermo Fisher Scientific, Denmark) containing 7.5 ml of growth medium (GM ...