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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Streptavidin-HRP (peroxidase-conjugated streptavidin biotin-binding protein, Thermo Scientific, 21130, 1:10000).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in TIM-4 binding buffer (PBS (ThermoFisher, Cat# 14190-144) + 0.5mM CaCl2 (VWR ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor™ 594 (conjugated streptavidin binding for biocytin, 1:400, Thermofisher, S11227).
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bioRxiv - Genetics 2021Quote: ... Poly (A) RNA was obtained from 2 μg of total RNA using the Dynabeads Oligo (dT)25-61005 Kit (Thermo Fisher, CA, USA), with two rounds of purification ...
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bioRxiv - Physiology 2020Quote: ... DNase I was used to eliminate genomic DNA and cDNA was synthesized from 2 μg RNA with RNA SuperScript III Reverse Transcriptase (Invitrogen, Life Technologies Incorporated). Expression of CTL1 ...
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bioRxiv - Molecular Biology 2021Quote: ... An adenylated adaptor was ligated to the 3’-end of the captured transcripts (Supplementary Table 3) by using a mixture of T4 RNA ligase and truncated T4 RNA ligase 2 (ThermoFisher Scientific and NEB), which served as a template for the reverse transcription primer (Supplementary Table 3) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was primed using a 2:1 molar ratio of random hexamer and polydT (Invitrogen) and reverse transcribed with Superscript IV (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... a maximum of 10 μg total RNAs was treated with 2 U Turbo DNase (Ambion) at 37 °C for 30 min followed by acid phenol extraction and ethanol precipitation ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA (2-5 μg) was resolved on a 15 % TBE-Urea gel (Invitrogen EC6885BOX). RNA of size between 17-25 nt was excised from the gel and extracted in 0.3 M NaCl overnight at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from 2×107 BCBL-1 cells using TRIzol™ (ThermoFisher 15596026), followed by DNase I treatment (ThermoFisher AM1907) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2 ul of a 1:20 dilution of ERCC RNA Spike-in mix (Invitrogen) was added to each sample.
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bioRxiv - Plant Biology 2019Quote: ... Reverse transcription was carried out using 2 µg of total RNA Multiscribe Transcriptase (Applied Biosystems). The equivalent of 20 ng of cDNA was used for quantitative PCR using gene specific primers for AtVip1 and AtVip2 and SYBRgreen as described in Desai et al. ...
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bioRxiv - Plant Biology 2019Quote: ... Purified RNA (2 μg) was incubated with DNase 1 (RNase-Free DNase, Thermo Fisher, USA) at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μg of RNA was digested with Turbo DNase (2 U/μl, # AM1907, ThermoFisher Scientific). 500 ng of DNase-digested RNA was tagged with G/I tails in a 10 μl reaction ...
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bioRxiv - Plant Biology 2021Quote: ... and 2 ug of total RNA were reverse transcribed using Superscript IV (Invitrogen, Carlsbad, USA). To confirm the plant response against R ...
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bioRxiv - Physiology 2021Quote: ... Samples (2 μl) were run on Qubit 3.0 using the Qubit RNA HS Kit (Invitrogen) to determine RNA quantification ...
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bioRxiv - Physiology 2021Quote: ... cDNA was synthesized using 2 ug RNA with RevertAid RT Kit (Thermofisher, catalog number K1691). Maxima SYBR Green/ROX qPCR Master Mix (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... and 2 µg of total RNA was depleted of contaminating DNA using TurboDNase (Applied Biosystems), using the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was removed from purified RNA by adding 2 µL of Turbo DNase (Invitrogen) in a 100 µL final volume using the provided buffer and incubating 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription was performed on 2 μg of total RNA using Superscript IV (Invitrogen Inc.) and real-time PCR was performed on Roche Light Cycler 96 using the specific primers with SYBR Green chemistry (see Table S5 and key resource table) ...
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bioRxiv - Genomics 2022Quote: ... UHRR or HEK-293T RNAs (5 μg) were DNased with 2 U TURBO DNase (Invitrogen) and purified using an RNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... We used 2 µg RNA for cDNA synthesis using a RevertAid cDNA synthesis kit (ThermoFisher) and measured gene expression using qRT-PCR (BioRad CFX system) ...
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bioRxiv - Plant Biology 2021Quote: ... 2 μg of total RNA was reverse transcribed with random primers using SuperScript II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... We extracted RNA from Caco-2 cells and ileum epithelium scrapings using TRIzol (Thermo Fisher) and used 1 µg for cDNA synthesis ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μg total RNA was reverse transcribed into cDNA using SuperScript VILO cDNA Synthesis (Invitrogen). Amplification was carried out for 40 cycles with annealing temperature of 60°C in 25 μl final volume ...
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bioRxiv - Microbiology 2021Quote: ... DNA contamination was removed by incubating the RNA extracts with 2 U TURBO DNase (Ambion) for 30min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Aliquots containing 2 μg total RNA were reverse transcribed to cDNA using Superscript III (Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was denatured at 80 °C for 2 min in Gel Loading Buffer II (Ambion) and size-separated on 0.5 x TBE (45 mM Tris-borate ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 2 mg total RNA using oligo (dT) and Superscript II (Invitrogen).
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bioRxiv - Plant Biology 2021Quote: ... A total of 2 μg RNA was used for cDNA synthesis using Superscript III (ThermoFisher). RNAse activity was inhibited by adding RiboLock (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... The total RNA (2 μg) was reverse transcribed using SuperScript II ribonuclease H-RT (Invitrogen) in 20 μl reactions following instructions.
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bioRxiv - Genomics 2022Quote: ... The remaining RNA (< 3 µg) was treated with 2 U TURBO™ DNase (Invitrogen, USA) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... 2 ug of total RNA were treated with TURBO DNA-free Kit (Thermo Fisher Scientific) to remove any residual genomic DNA ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μg of total RNA from each sample was reverse transcribed using Superscript IV (InVitrogen) and a mixture of 20 μM random hexamer and 20 μM oligo(dT)20 primers ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized using 2 μg of total RNA with SuperScript II reverse transcriptase (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: 1.5-2 μg total RNA was diluted 1:1 in 2x sample buffer (Invitrogen™), loaded onto 7.5% TBE-Urea PAGE gels ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of DNase-treated RNA was subjected to cDNA synthesis using Superscript III (Invitrogen) reverse transcriptase and a combination of both random hexamers and oligo dT primers (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was collected from 1-2 million UOK262 cells using Trizol reagent (ThermoFisher #15596026) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was collected from 1-2 million UOK262 cells using Trizol reagent (ThermoFisher #15596026) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... 8 µg of total RNA was treated with 2 U of TURBO DNase (Life technologies) for 20 min at 37°C followed by a 5 min incubation step at room temperature with the DNase Inactivation Reagent ...
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bioRxiv - Pathology 2023Quote: ... and/or 2 mM SYTO RNA-Select Green Fluorescent Cell Stain Kit (Thermo Fisher Scientific) for 1 h at 37°C to stain RNAs inside EV ...
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bioRxiv - Neuroscience 2022Quote: ... total RNA from Cohort 2 was isolated using TRIzol reagent (Life Technologies, Scoresby, VIC, Australia) and RNeasy mini kits (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... 2 ug of DNase-treated RNA was reverse transcribed into cDNA using Superscript III (Invitrogen) with random hexamers (Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... RNA integrity was confirmed by gel electrophoresis on E-gel EX 2% agarose gels (Thermofisher).
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bioRxiv - Biochemistry 2023Quote: ... cDNA was synthesised from 2 μg of RNA using TaqMan Reverse Transcription Reagent (Applied Biosystems) and random hexamer primers ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg total RNA was reverse transcribed into cDNA using SuperScript VILO cDNA Synthesis (Invitrogen). Amplification was carried out for no more than 28 cycles with annealing temperature of 60°C in 25 μl final volume ...
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bioRxiv - Cell Biology 2024Quote: ... followed by cDNA synthesis from 2 µg total RNA using SuperScript III reverse transcriptase (Invitrogen) and a mixture of 80 pmol random primers (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... indicated SARS-CoV-2 Z-RNAs) was incubated with Lipofectamine 2000 transfection reagent (Invitrogen, 11668019), for 15 mins at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
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bioRxiv - Microbiology 2021Quote: ... incubated in primary antibody (anti-SARS-CoV-2 Spike, which detects the S2 subunit of SARS-CoV-2 S (Invitrogen, PA1-41165), anti-GAPDH (proteintech ...