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Citations for Thermo Fisher :
601 - 650 of 3609 citations for Super Resolution microscope since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... connected to a Dionex UltiMate 3000 UHPLC system and a Q-Exactive Orbitrap high resolution mass spectrometer (Thermo Fisher Scientific) equipped with a heated electrospray ionization (HESI-II ...
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bioRxiv - Plant Biology 2024Quote: ... The mass spectra were acquired using a Q-exactive Orbitrap high-resolution mass spectrometer: Fourier-transform mass spectrometer (FT-MS) (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... High-resolution ESI-MS spectra were performed with LTQ-XL equipped with an Ultimate 3000 HPLC system (Thermo Fisher Scientific) mass spectrometer.
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bioRxiv - Pathology 2024Quote: ... LC–MS/MS technique was used in a high-resolution mass spectrometer Q Exactive HF system (Thermo Fisher Scientific, USA). Data was collected and further analyzed using the R 4.1.1 DeSeq2 package ...
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bioRxiv - Biochemistry 2024Quote: Phosphopeptides were directly analyzed following reconstitution in 0.6% acetic acid by autosampler injection onto a liquid chromatography system coupled to a high-resolution mass spectrometer (Thermo Scientific UltiMate 3000 RSLCnano ...
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bioRxiv - Genetics 2024Quote: ... samples were diluted to equal concentrations and heated at 70°C for 10 min before resolution on 12% or 10-20% SDS-PAGE gels (Invitrogen). Resolved proteins were transferred to nitrocellulose membrane in CAPS transfer buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with Super Signal Chemiluminescent Substrate (Thermo 34580) for 5 min with gentle rotation and imaged by iBright FL1000 (Invitrogen).
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bioRxiv - Developmental Biology 2022Quote: ... Cryosectioning of samples was performed using a Leica CM1850 cryostat and 20μm thick transverse sections were collected in glass slides (Super Frost Ultra Plus, #11976299, Thermo Scientific) for Phalloidin (#A12379 ...
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bioRxiv - Cell Biology 2020Quote: ... After 3×15 min washes in TBST signal was detected using Super Signal West Pico/Femto Chemiluminescence Substrate (Thermo Fisher) and a ChemiDoc Touch imaging system (Bio-Rad).
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bioRxiv - Plant Biology 2019Quote: ... cDNA was synthesized from 5 µg RNA using Super Script® II Reverse Transcriptase in a 20 µl system (Invitrogen). Quantitative RT-PCR was performed on a CFX384 Touch™ Real-Time PCR Detection System using SYBR Green dye (iQ™ SYBR® Green Supermix ...
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bioRxiv - Cancer Biology 2020Quote: ... cells from the previous steps were barcoded by cotransfecting PB-EF1α-Puro-V8.2 library and Super PiggyBac Transposase plasmid (SBI #PB210PA-1) at a 1:10 molar ratio using Lipofectamine 3000 (Thermofisher). Barcoded cells were puromycin-selected for 7 d ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 units of RNase OUT (40 unit/μL) and 10 units of Super Script III reverse transcriptase (200 unit/μL) (Invitrogen), to make a total volume of 20 μL ...
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bioRxiv - Microbiology 2020Quote: The sequence containing the first 186 amino acids of LacZ (LacZ186) and the super-folder GFP (sfGFP) open reading frame (78) expressed by J23119 constitutive promoter was obtained from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-PCR was performed using the Super-Script III One-Step RT-PCR System with Platinum Taq (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... were used at 1:10,000 dilution to detect the bait-prey complex and blot was developed by Super signal west pico chemiluminescent substrate (Thermofisher).
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bioRxiv - Developmental Biology 2022Quote: Total RNAs were isolated from sorted skin cells prepared by TriZol method and used for reverse transcription by Super script III (Invitrogen). The primers used were as follows ...
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bioRxiv - Immunology 2022Quote: ... then RNA was converted to cDNA with an Invitrogen Super Script IV First Strand synthesis System kit (18091050 from Invitrogen). Final cDNA concentration was checked with a spectrophotometer ...
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bioRxiv - Developmental Biology 2021Quote: ... The signal in both the blots were detected using Super Signal West Dura Extended Duration Substrate (ThermoFisher Scientific, Cat. #34076). Images were captured using the UVP Imaging System ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x 105 ESCs were co-transfected with 1.5 μg wild-type or mutants of PB-ATRX-HA-Neo and 1.5 μg Super PiggyBac transposase using Lipofectamine 3000 (Thermo Fisher). After two days of transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNAs were obtained from 1 µg of total DNase-treated RNA in a 20 µl reaction containing 200 units of Super-Script III Reverse Transcriptase (Invitrogen) and 50 pmol oligo (dT)20 (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... selected RNAs were primed with oligo dT and reverse transcribed using a Super Script III First Strand cDNA Synthesis kit (Invitrogen). Primers were designed against selected lncRNAs from the 5’-ends for most transcripts except for RP11-574K11.28 and GAS5-838 where primers were designed against the 3’-ends ...
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bioRxiv - Biophysics 2021Quote: ... Total RNA from the PBLs was extracted and reverse transcribed into cDNA using a Super-ScriptTM III FIRST-Strand SUPERMIX Kit (Invitrogen). The VHH encoding sequences were amplified with two-step enriched-nested PCR using VHH-specific primers and cloned between PstI and BsteII sites of pMECS vector ...
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bioRxiv - Cell Biology 2019Quote: RNA from asexual blood stages was isolated as described above and 2 µg of each total RNA sample was used as template for cDNA synthesis using the Super Script IV First-Strand Synthesis System (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR was performed in triplicates from 5ng of RNA per reaction using Platinum SYBR Green qPCR Super Mix-UDG w/ROX (Invitrogen) on a ViiA7 qPCR detection system (Life Technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... primed reverse transcription of first-strand cDNA synthesis was carried out with 7 mg total RNA using Super ScriptTM III (Invitrogen). Equal volumes of each first-strand reaction were amplified with gene-specific primer pairs ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed with PBS-T thrice before visualization with enhanced chemiluminescence via the super signal west femto maximum sensitivity substrate (ThermoFisher) on ChemiDoc XRS+ (Biorad ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extracted from mouse ovaries or 50 immature oocytes was used for cDNA synthesis using the Super Script III First Strand Synthesis System (Invitrogen). The full length of Mad2 mRNA was amplified with the cDNA and primer sets specific to Mad2 ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated from ~1 ug RNA per condition with random hexamers using Super Script IV First-Strand cDNA Synthesis Reaction (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Equal volumes of RNA from each fraction was then reverse transcribed using super script III reverse transcriptase (Thermo Fisher Scientific) and polydT(20 ...
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bioRxiv - Immunology 2021Quote: ... reverse transcription (RT) of 200 ng of RNA was carried out using the Super Script II reverse transcriptase kit (ThermoFisher). Real time quantitative PCR (RT-qPCR ...
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bioRxiv - Biochemistry 2022Quote: ... and the super was added to 15 µL of washed Pierce anti-DYKDDDDK (FLAG) magnetic agarose beads (Thermo Fisher Scientific). The mixture was rotated 1-2 hours at 4°C and washed 3x with PBS + 0.1% NP-40 + 150 mM NaCl ...
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Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platformsbioRxiv - Developmental Biology 2022Quote: ... The snap-frozen tissue was then bio-pulverized under super-cooled conditions and total RNA was prepared from these cells using TRIzol reagent (Invitrogen) followed by RNeasy Mini kit (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: ... treated-RNAs were retro-transcribed using random hexo-nucleotides or reverse gene-specific primers and the Super-Script II enzyme (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR analyzes were done in triplicate using 2ng/ul cDNA with Platinum SYBR Green qPCR Super Mix (Invitrogen) in an ABI Prism 7000 thermocycler (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... Stably expressing cell-lines were generated by transfecting 1 μg of the HaloTagged TF Piggybac construct together with 1 μg of the Super Piggybac transposase plasmid (gifted from the Tjian Lab) using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The magnetic force field created by the magnet tip was first characterized by pulling super-paramagnetic 1 μm Dynabeads (MyOne Streptavidin C1; Thermofisher) in a viscous test fluid (80% glycerol ...
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bioRxiv - Developmental Biology 2022Quote: ... all cell aggregates were collected and individually transferred to a new 96-well U-bottom plate with a super-low cell attachment surface in 100 µl of E6 medium (A1516401, Gibco) with Normocin (concentration 100 µg/ml) ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated with Super Signal Chemiluminescent Substrate (Thermo 34580) for 5min with gentle rotation and imaged by iBright FL1000 (Invitrogen). For HIF-1a ...
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bioRxiv - Biochemistry 2022Quote: ... was created by sticking the PLL-PEG passivated side of the coverslip to a glass slide (Super Frost, Thermo Scientific) using a double-sided adhesive tape ...
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bioRxiv - Molecular Biology 2023Quote: ... selected RNAs were primed with oligo dT and reverse transcribed using a Super Script III First Strand cDNA Synthesis kit (Invitrogen). Primers were designed against selected transcripts from the 5’-ends and spike in controls as done earlier ...
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bioRxiv - Bioengineering 2023Quote: ... At least 320 ng RNA per sample was synthesized for cDNA using Super Script III Reverse Transcriptase Kit (Thermo Scientific). Gene expression was performed using Applied Biosystems Step One Plus Light Cycler instrument using SYBR green master mix (Thermo Scientific).
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bioRxiv - Genomics 2023Quote: ... approximately 1.5 million wildtype MCF10A cells were incubated with 1 µg PiggyBac-NLS-ZapCV2 and 200 ng Super PiggyBac Transposase (Systems Biosciences #PB210PA-1) and electroporated using a Neon Electroporation System (ThermoFisher). Cells were plated into antibiotic-free media (the same composition as above ...
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bioRxiv - Molecular Biology 2024Quote: ... After three washes with TBST the protein bands were detected with Super Signal West Pico Substrate (34078, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... in 5% milk and 0.1% Tween 20 in TBS and visualized using Super Signal West Pico or Femto Chemiluminescent Substrate (Pierce, Thermo Fisher) on a ChemiDoc-MP imaging system (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Fluorescence microscope was performed using microscope of EVOS™ M5000 (Invitrogen).
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bioRxiv - Cell Biology 2019Quote: ... The X-Y resolution of N-SIM was calibrated to 120-150 nm using 20 nm FluoSphere beads (Thermo Scientific, F887) in 580 nm laser ...
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bioRxiv - Biochemistry 2021Quote: ... MS data were acquired in full-scan mode at high resolution on a hybrid Orbitrap Elite (Thermo Fisher Scientific, Bremen, Germany). The system was operated at 240,000 resolution (m/z 400 ...
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bioRxiv - Systems Biology 2021Quote: LC-MS/MS experiments were performed on the Dionex Ultimate 3000 UHPLC system coupled with the high resolution nano-ESI Orbitrap-Elite mass spectrometer (Thermo Scientific). Each sample was reconstituted in 10 μL loading solution composed of 0.1 % v/v formic acid ...
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bioRxiv - Microbiology 2020Quote: ... Samples were analyzed using an HPLC-MS system consisting of a Dionex UHPLC coupled by electrospray ionization (ESI; negative mode) to a hybrid quadrupole-high-resolution mass spectrometer (Q Exactive orbitrap, Thermo Scientific) operated in full scan mode ...
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bioRxiv - Microbiology 2021Quote: ... Ions were generated by heated electrospray ionization (HESI; negative mode) and quantified by a hybridquadrupole-high-resolution mass spectrometer (Q Exactive orbitrap, Thermo Scientific). MS scans consisted of full MS scanning for 70-1000 m/z from time 0–18 min except MOPS m/z of 208-210 was excluded from 1.5-3 minutes ...