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Citations for Thermo Fisher :
1 - 50 of 6119 citations for Sodium 40 wt. % dispersion in oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 6-8-week-old WT mice were randomly selected to receive 2% dextran sodium sulfate (DSS) 40-50,000 MW (Affymetrix, Santa Clara, CA) in drinking water for 7 days followed by normal drinking water afterwards ...
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bioRxiv - Neuroscience 2021Quote: ... 40 ng/mL sodium selenite (#31966-021, Invitrogen), 60 µg/mL N-acetyl-cystein (#A9165 ...
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bioRxiv - Bioengineering 2020Quote: ... was blended together with a 40 wt% mineral suspension of phosphoric acid (Fisher Scientific) and calcium hydroxide (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Organoids were passaged every 4–6 days by dispersion with TrypLE Express (Invitrogen, Carlsbad, CA) [13] ...
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bioRxiv - Microbiology 2022Quote: ... The solid dispersion was further dried overnight using a vacuum oven (Fisher Scientific, Waltham, MA). The NCL-EPO solid dispersions ...
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bioRxiv - Bioengineering 2023Quote: ... The dispersion was transferred into a dialysis bag with a MWCO of 7 kDa (ThermoFisher, USA) for dialysis against deionized water to remove organic solution and stored at 4 C° for further use ...
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bioRxiv - Cell Biology 2022Quote: ... Organoids were passaged every 4 to 6 days by dispersion with TrypLE Express (Invitrogen, Carlsbad, CA, USA) and re-embedded in Matrigel-GFR.
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bioRxiv - Biochemistry 2020Quote: ... a DMSO/NaOH slurry was first prepared by dissolving 300 µL 50 wt % aqueous solution sodium hydroxide (Acros Organics # 25986025) in 600 µL methanol (Fisher Scientific # AF544) ...
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bioRxiv - Cell Biology 2022Quote: ... MEF ANT Triple KO cells were grown in the same media as MEF WT cells with the addition of 1mM Sodium Pyruvate (Gibco) and 25mg/500mL Uridine (Sigma Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... hippocampi from WT and App KI pups (P0-P2) were isolated in ice-cold HBSS (1 mM sodium pyruvate, 1% HEPES; Gibco) and trypsinised (0.25% trypsin ...
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bioRxiv - Microbiology 2021Quote: Enriched β-GD (40 mg) was reduced with sodium borodeuteride (20 mg/ml) (ACROS Organics, cat.no. 194950050) in 1 M ammonium hydroxide for 90 minutes at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... embryos were placed in halocarbon oil and injected using a microinjection setup with ROCK inhibitor (H-1152, 40 mM, Invitrogen), Embryos were immersed in halocarbon oil for spinning disk imaging.
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bioRxiv - Microbiology 2022Quote: ... centrifuged at 1500 rpm for 5 minutes at 4°C and lysed with RIPA lysis buffer (25mM Tris HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) (ThermoFisher) supplemented with protease inhibitor cocktail (Sigma) ...
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bioRxiv - Immunology 2023Quote: Cells or extracted mitochondria were lysed by sonication in RIPA lysis buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, ThermoFisher) containing Minitab protease inhibitors (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... 1.5 wt/wt % Me-HA was crosslinked in phenol-free DMEM (Gibco) with bifunctional thiol dithiothreitol (DTT ...
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bioRxiv - Bioengineering 2020Quote: ... A total volume of 60 µL composed of approximately 20 µL of drops and 40 µL of oil were collected in 100 µL PCR tubes (Applied Biosystems). After the drops were collected ...
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bioRxiv - Biochemistry 2020Quote: ... Tissues were finally suspended in 1% (w/v) low-melting agarose at ~40°C mixed with Slowfade Diamond oil (Molecular Probes) on a slide ...
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bioRxiv - Neuroscience 2021Quote: ... E17.5 cortical tissue was dissected in ice-cold dissection medium (HBSS medium [Invitrogen], supplemented with 100 mM sodium pyruvate [Invitrogen], 20% (wt/vol) glucose [Merck Millipore] and 1 M Hepes [SigmaAldrich] ...
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bioRxiv - Microbiology 2020Quote: ... were mixed with 60 μL of 20× saline-sodium citrate (SSC) buffer (3 M NaCl, 0.3 M trisodium citrate) and 40 μL of 37% formaldehyde (Invitrogen) and incubated at 65 °C for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Cortical samples were stored at -80 °C until homogenization in RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Fisher) containing cocktails of protease and phosphatase inhibitors ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The striatal and hippocampal samples were stored at -80°C until homogenization in RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Fisher) containing cocktails of protease and phosphatase inhibitors ...
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bioRxiv - Genetics 2023Quote: ... assay buffer (150 mM Tris pH 8.0, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% NP-40; Thermo Fisher Scientific), supplemented with protease inhibitor cocktail (Roche) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were placed in a 100 mL beaker with 40 mL of 1% sodium dodecyl sulfate (SDS) (Thermo Scientific) solution and agitated in an orbital shaker at 100 RPM at ambient temperature for 3 days ...
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bioRxiv - Cell Biology 2023Quote: Protein from 2×106 cells was harvested on ice after 1 PBS wash using the Pierce RIPA lysis and extraction buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Life Technologies) containing fresh protease and phosphatase inhibitors (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... or ∼10 mg of tissue were lysed by sonication in RIPA buffer (25mM Tris-HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Scientific) containing 0.5 mM phenylmethyl sulphonyl fluoride ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks and nasal washes were diluted 10-fold in 1× minimum essential medium (MEM) (10% 10× minimal essential medium [Gibco], 2 mM L-glutamine, 0.1% of sodium bicarbonate [wt/vol; Gibco], 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... The CNS tissue was homogenized and dissociated in 100 μl of Dispersion medium (Hank’s Balanced Salt Solution (1xHBSS, Life Technologies, 14170088) supplemented with Phenylthiourea (Sigma-Aldrich P7629 ...
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bioRxiv - Microbiology 2022Quote: NCL-EPO-NPs dispersion (0.5 ml) was transferred to the Amicon Ultra-0.5 device (3 kDa membrane; Fisher Scientific, Waltham, MA). The Amicon-Ultra centrifugal filter containing NCL-EPO-NPs was centrifuged for 15 min at 14000 rpm (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Fluorescent liposomes and EVs were obtained by incubating the vesicle dispersions overnight with β-Bodipy TM FL C12-HPLC (emission wavelength 512 nm, Thermofisher) and 18:1 Cyanine-5-Phosphatidylethanolamine (Cy5 ...
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bioRxiv - Physiology 2024Quote: ... It was achieved by initial dispersion of isolated pancreatic islets with trypsin and subsequent sonication (sonic dismembrator model 705, ref: 86853K-09-15, Thermo Fisher). After the final centrifugation step in the pancreatic islet dispersion ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclease S1 cleavage was carried out for 15 minutes at room temperature in 1× Reaction Buffer (40 mM sodium acetate pH 4.5, 300 mM NaCl and 2 mM ZnSO4; Thermo Scientific), and 0.1 μg of total yeast tRNA (Ambion ...
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bioRxiv - Neuroscience 2020Quote: ... buffer (25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, and 0.1% SDS) (ThermoFisher Scientific, #89900) containing protease (MilliporeSigma ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Pelleted cells were subjected to lysis in an appropriate volume of Radio Immuno Precipitation Assay buffer (RIPA; 1% NP-40; 0.5% Sodium Deoxycholate; 0.1% SDS) supplemented with 1X protease and phosphatase inhibitor (Thermo Scientific, 1860932) for 15 minutes on ice ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, supplemented with Halt protease inhibitor cocktail [Thermo Fisher Scientific]) to obtain whole-cell lysates or lysed using the NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell pellets were lysed with 3-5x packed cell volume of RIPA buffer (1x PBS, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with protease and phosphatase inhibitors (Thermo Scientific, A32959). Cells were lysed on ice for 30 minutes with periodic vortexing every 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: Cells were washed twice with PBS and lysed with RIPA buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Fisher; 89901) supplemented with 1 mM EDTA (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected with an siRNA or a miRNA mimic/inhibitor and then collected and lysed 48 or 72 hours post transfection using RIPA buffer (1x PBS, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with protease and phosphatase inhibitor (ThermoFisher Scientific, A32959). Total protein concentration was determined using the Bradford quantification assay (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Dephosphorylated mRNA fragments were precipitated in 100% ethanol with 40 μl 3 M sodium acetate and 1 μl linear acrylamide (AM9520, Ambion) at -20°C for >16 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein was isolated from these brains using RIPA buffer (25 mM Tris HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Scientific #89900) supplemented with 1X Protease Inhibitor Cocktail (Roche # 5892791001 ...
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bioRxiv - Microbiology 2024Quote: Protein was isolated by scraping cells with 100 uL of Pierce RIPA Lysis and Extraction Buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Fisher Scientific). Lysates were incubated on ice for 30 minutes and sonicated 2X for 15 seconds to lyse cells ...
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bioRxiv - Biochemistry 2024Quote: Cell pellets were lysed in 90uL of cold RIPA lysis buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Scientific, 89901), protease inhibitors (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... 0.5% (wt/vol) AlbuMAXII (Invitrogen) and 10 μg/mL gentamycin ...
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bioRxiv - Biochemistry 2022Quote: ... 2% (wt/vol) B27 (GIBCO), and 1% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and 40-50 μg of proteins were loaded per lane on NuPAGE 4-12% (wt/vol) Bis-Tris gels (Invitrogen; for all genes except CDH23) or 3-8% Tris-Acetate gels (Invitrogen ...
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bioRxiv - Genomics 2023Quote: Cell lysates were prepared in chilled RIPA buffer (25 mM Tris-HCL pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Scientific, Rockford, IL) containing protease inhibitor (complete Mini ...
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bioRxiv - Bioengineering 2023Quote: (GT)15-SWCNT-containing (1.0 mg/L) alginate-acrylamide gels were prepared by modifying a previously published procedure.40 Sodium alginate (116.7 mg, Thermo Fisher Scientific) and n-isopropylacrylamide (1524.94 mg ...
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bioRxiv - Physiology 2020Quote: ... The GeneChip WT Expression Kit and GeneChip WT Terminal Labeling and Controls Kit (Thermo Fisher Scientific) were used to generate amplified and biotinylated sense-strand cDNA from expressed transcripts (250 ng of total RNA) ...
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bioRxiv - Microbiology 2023Quote: ... and 1% (wt/vol) ultraglutamine (Gibco) at 37°C and 5 % (vol/vol ...
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bioRxiv - Microbiology 2022Quote: ... bacterial were incubated in hot phenol lysis solution (1% SDS, 2 mM EDTA, 40 mM sodium acetate in acid phenol (Invitrogen #15594-047)) for 45 minutes at 65 °C ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were lysed in a hot (60 °C) phenol solution (2 mM EDTA, pH8, 1% SDS, 40 mM sodium acetate in acid phenol (Invitrogen #15594-047)) ...