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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Siglec 6 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: Human 293F cells were maintained at 37°C with 5-8% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Biochemistry 2023Quote: Human Embryonic Kidney Cells 293 (HEK293T cell) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Scientific™) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: Freestyle 293-F cells were grown in Freestyle 293 expression medium (Thermo Fisher) in suspension culture ...
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bioRxiv - Genomics 2024Quote: Freestyle 293-F cells were grown in Freestyle 293 Expression Medium (ThermoFisher Scientific) at 37°C and 8% CO2 while shaking at 135 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... Freestyle 293 Expression Medium and Expi 293 Expression Medium were purchased from Gibco, along with Expi293F cells and the ExpiFectamine 293 Transfection Kit ...
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Cell Biology 2020Quote: ... Human IL-6 and IL-8 ELISAs were from Thermofisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Human Interleukin 6 concentrations were obtained via ELISA (Invitrogen, EH2IL6). ELISA was performed according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: The IL-6 Receptor (Soluble) Human ELISA Kit (Invitrogen; BMS214) was used as per the manufactures instructions to quantify soluble IL-6R expression in day 14 MGL and day 18 NPC vehicle/treated cell culture media ...
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bioRxiv - Neuroscience 2023Quote: The IL-6 Receptor (Soluble) Human ELISA Kit (Invitrogen; BMS214) was used to quantify soluble IL-6Ra expression in cell culture media ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Developmental Biology 2021Quote: ... and HEK-A (ThermoFisher) were maintained in Dulbeccos Modified Medium (DMEM ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... and HEK-A (ThermoFisher) were maintained in Dulbeccos Modified Medium (DMEM ...
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bioRxiv - Bioengineering 2020Quote: HEK 293F cells (Invitrogen) were cultured in Freestyle medium (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-A (ThermoFisher) were maintained in Dulbeccos Modified Medium (DMEM ...
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bioRxiv - Biophysics 2020Quote: ... HEK 293F cells (Invitrogen) were transfected with the four plasmids and harvested after 60 hours ...
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bioRxiv - Cell Biology 2022Quote: HEK-293FT cells (Invitrogen) were cultured in 1x DMEM ...
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bioRxiv - Immunology 2022Quote: HEK-293T cells (Invitrogen) were transfected using SARS-CoV-2 S expression plasmid expression vector and lipofectamine (thermofisher ...
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bioRxiv - Developmental Biology 2019Quote: HEK cells were grown in 6 well plates and transfected with 4µg of each DNA with lipofectamine 2000 (Invitrogen) for 6hrs according to manufacturers instructions.
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bioRxiv - Immunology 2019Quote: ... and the ELISPOT plate was incubated with biotinylated goat anti-human IgG Fc (Invitrogen), followed by HRP-conjugated avidin (Vector Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated antibody specific for the Fc region of human IgG (Invitrogen) was used as secondary antibody to detect antibody binding in ELISA experiments.
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled anti-human IgG Fc antibody (Catalog# 31413, Thermo Fisher) was added to each well and incubated for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... nanovials were modified with biotinylated goat anti-human IgG Fc antibody (Thermo Fisher Scientific). Samples were resuspended in DMEM and seeded in a well plate ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated Abs specific for the Fc region of human IgG (Invitrogen) were used as secondary antibodies to detect antibody binding in ELISA experiments ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Protease susceptibility measurements were performed in biological replicates (n = 2 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Titrations were performed in biological replicates (cultures from independent colonies grown on separate days ...
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bioRxiv - Immunology 2020Quote: ... followed by addition to the Fc-chimeric human ACE2-coated MaxiSORP ELISA plate (Nunc) for an hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Human JAGGED1-Fc (Martin et al., 2023) was transfected into Expi293F cells (ThermoFisher, A14527) using FectroPro (Polyplus ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human Fc chip was regenerated to baseline using IgG Elution Buffer (Thermo Scientific) and the process was repeated for all humAbs in the panel ...
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bioRxiv - Immunology 2020Quote: ... cells were pre-treated with 20µg/ml Siglec-1 mAb (Invitrogen, HSn 7D2) for 30 minutes before HIV was added.
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bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293T overexpressing the human ACE2 were kindly provided by Integral Molecular Company and maintained in DMEM (Invitrogen) with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in DMEM+GlutaMAX-I (Thermo Fisher Scientific) supplemented with heat-inactivated 8% FBS.
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bioRxiv - Microbiology 2021Quote: ... HEK-293T overexpressing the human ACE2 were kindly provided by Integral Molecular Company and maintained in DMEM (Invitrogen) with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... human embryonic kidney (HEK) 293T and Vero cells (ATCC) were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) (Gibco), supplemented with 10% fetal calf sera (FCS ...
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bioRxiv - Microbiology 2023Quote: ... Human embryonic kidney 293T cells (HEK 293T) were maintained in DMEM GlutaMAX (Dulbecco’s Modified Eagle Medium, ThermoFisher Scientific) supplemented with decomplemented FCS 10% (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Cell Biology 2021Quote: ... FreeStyle™ 293-F cells were grown in FreeStyle™ 293 Expression Medium (Gibco) under constant agitation at 37 °C with 5% CO2 in a humidified incubator.
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bioRxiv - Biochemistry 2021Quote: ... FreeStyle 293-F cells were grown in suspension in FreeStyle 293 Expression Medium (Gibco).
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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A quantitative analysis of the interplay of environment, neighborhood and cell state in 3D spheroidsbioRxiv - Systems Biology 2020Quote: T-REx-293 cells (Invitrogen) and DLD1 cells (Flp-In T-Rex DLD-1 ...
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bioRxiv - Microbiology 2021Quote: ... T-REx-293 cells (Invitrogen) were grown in DMEM supplemented with blasticidin (10 µg/mL ...
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bioRxiv - Microbiology 2021Quote: T-REx-293 cells (Invitrogen), which constitutively expresses the Tet repressor (TetR ...