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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Septin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Dried peptides were resuspended in DI water and then quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Plant Biology 2024Quote: ... Labeled peptides were separated into 6 fractions with Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher, Cat. no. 84868) and dried in a Speed Vac at 45 °C for 3.5 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The deuterium exchange levels were determined by subtracting the centroid mass of undeuterated peptide from the centroid mass of deuterated peptide using HDExaminer (Version PD1.4, Thermo-Fisher Scientific, USA).
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bioRxiv - Cell Biology 2023Quote: ... with a monoclonal antibody against B5 (19C2, rat, 1:1000; (Schmelz et al. 1994)) followed by an Alexa Fluor 647 anti-rat secondary antibody (Invitrogen; 1:1000 in blocking buffer) prior to permeabilization of the cells with detergent ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were stained with a monoclonal antibody against B5 (19C2, rat, 1:1000 (Schmelz et al, 1994)) followed by an Alexa Fluor 647 anti-rat secondary antibody (Invitrogen; 1:1000 in blocking buffer). Cells were then permeabilized with 0.1% Triton-X/PBS for 3 min ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS before staining with primary antibodies diluted in blocking buffer and a mixture of 1:1000 of Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Invitrogen), 1:5000 Dapi (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed X3 with PBS and incubated in the dark for 60 minutes at RT with Alexa Fluor 594 goat anti-rabbit IgG (H+L) (1:500 dilution in blocking buffer) (Invitrogen). Coverslips were washed X3 with PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Developmental Studies Hybridoma Bank) was used at 1:30 in blocking solution and goat α-mouse Alexa568 secondary antibody (Invitrogen) at 1:1,000 in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... Reactions with primary antibodies were performed in blocking buffer containing rabbit anti-GFP (1:1000, Invitrogen, Carlsbad, CA, USA, A11122) and/or goat anti-c-Fos (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated in Odyssey Blocking Buffer containing HRP-conjugated anti-rabbit IgG (H+L) (1:2000, Life Technologies 31462) or anti-mouse IgG (H+L ...
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bioRxiv - Bioengineering 2020Quote: ... The plate was in-cubated for 1 h at room temperature with Pierce Protein-Free Blocking Buffer (Thermo Fisher Scientific) and again washed with PBS three times ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with PBS for 10 min followed by incubation with secondary antibodies diluted 1:500 in blocking buffer: goat anti-Chicken Alexa Fluor®546 (RRID: AB_2534097, ThermoFisher) and Goat anti-Rabbit Alexa Fluor®488 (RRID:AB_2534096 ...
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bioRxiv - Neuroscience 2021Quote: ... secondary antibodies diluted 1:1000 in blocking buffer (donkey anti-mouse Alexa 488, donkey anti-rabbit Alexa Fluor 555 or 647, Invitrogen) were applied for 4 hours at room temperature with agitation ...
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bioRxiv - Cell Biology 2021Quote: ... cardiomyocytes were incubated with the secondary antibody diluted in a blocking buffer solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse 594 (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was incubated in blocking solution containing secondary antibodies for 24 hr (streptavidin AlexaFluor conjugate, Invitrogen; 1:250 goat anti-mouse AlexaFluor conjugate, Invitrogen). Following staining ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217, 1:1,000; chicken anti-GFP, Life Technologies A10262 ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:100 in blocking buffer and the secondary antibody (Anti-mouse IgG conjugated with Alexa 635, Goat IgG - Invitrogen) diluted 1:300 in blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... then incubated for two hours with Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody against phospho-AKT and total-AKT and Alexa Fluor 596-conjugated goat anti-mouse secondary antibody for PRMT5 (diluted 1:100 in blocking buffer, Invitrogen) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSC-CMs were washed 3 times with PBS 1X and incubated with the secondary antibodies diluted in blocking solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse Alexa Fluor 488 (1:400 ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... goat anti-mouse 488 or goat anti-rabbit 488 diluted in blocking buffer (A11034 and A32731, Thermo Fisher; 1:400) for 75 min at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... in 1X PBS before staining with primary antibodies diluted at 1:400 ratio in 5% NGS blocking buffer and a mixture of 1:1000 of Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Invitrogen) and 1:5000 DAPI (Sigma-Aldrich in 1X PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The next day sections were washed three times for 10 minutes in PBST followed by a 2-hour incubation in the dark with secondary antibodies in blocking solution (1:1000 donkey anti-rabbit Alexa Fluor 594 (ThermoFisher Scientific ...
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Cryptococcus neoformans capsule regrowth experiments reveal dynamics of enlargement and architecturebioRxiv - Microbiology 2021Quote: ... cells were spun down and washed with PBS then resuspended in 1mL blocking solution with either 1:500 Goat anti-Mouse IgM Heavy Chain Secondary Antibody conjugated to AlexaFluor 488 (Invitrogen), 1:500 Rabbit anti Mouse IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with secondary antibodies diluted in blocking buffer for 45 min (1:1000 Alexa-fluor 546 conjugated to mouse or rabbit, Invitrogen) followed by three PBS washes ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor secondary antibodies were diluted to 1:500 in blocking solution and included: 488 goat anti-rat (Invitrogen #A21212), 488 goat anti-chicken (Invitrogen #A11039) ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed three times in PBS and incubated in blocking solution containing an Alexa 488 conjugated secondary antibody (1:1500; Invitrogen), washed in PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were incubated overnight at 4°C in blocking-permeabilizing solution containing antibodies anti-PAX6 (mouse, 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed with PBS-T (3 × 5 min) and incubated with fluorophore-conjugated secondary antibodies (1:500 in blocking buffer, Invitrogen). Hoechst 33258 (Invitrogen ...
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bioRxiv - Systems Biology 2019Quote: ... sections were washed with blocking solution and incubated with DAPI (1 μg/ml in PBS, Thermo Fisher Scientific Inc.; D1306) to stain DNA for 10 min at RT before they were covered with coverslips using Fluoromount-G® (SouthernBiotech ...
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bioRxiv - Neuroscience 2021Quote: ... slices were washed 3 times in PBS and incubated for a further hour at room temperature in diluted blocking solution containing 1:500 goat anti-mouse Alexa488-conjugated IgG secondary antibody (#A28175, RRID:AB_2536161, Invitrogen, Carlsbad, CA) and 1:5000 Hoechst stain (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed 3 times in the blocking solution and incubated in Alexa Fluor 594-conjugated (red) goat secondary antibody (1:500; Invitrogen) for 2 hr.
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bioRxiv - Microbiology 2022Quote: ... Goat anti-hamster IgG (H+L) HRP conjugated secondary antibody (SeraCare 5220-0371) was diluted 1:3,000 in casein in PBS blocking buffer (Thermo Fisher), and 100 μL was added to the wells and allowed to incubate for one hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were then stained using a rat anti-tubulin primary antibody solution (diluted 1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4°C overnight incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were rinsed with PBS-TB (PBS supplemented with 0,05% Tween and 1% BSA) and incubated for 30 minutes with 200μl Superblock blocking buffer (Thermo Scientific, #37515). Antibody incubation was split into two steps ...
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bioRxiv - Cell Biology 2022Quote: ... The following day slides were washed thrice with blocking buffer for 15 min and incubated for 1 h at RT in secondary antibodies and Hoechst 33342 (Invitrogen) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed in blocking buffer with Hoechst nuclear stain (1:10,000) and mounted with Prolong Gold Antifade (Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... the pellet was resuspended in 90 μl of Secondary antibody solution (Blocking buffer + 1:500 Goat anti-rabbit IgG AlexaFluor 647 antibody [#A27040, Invitrogen]) and the samples were incubated at room temperature for 45 min in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer, Ref# A21202, Invitrogen) at 4 °C for 24 hr ...
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bioRxiv - Neuroscience 2023Quote: ... in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer, Ref# A11120, Invitrogen). The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... sections were then incubated for 2h at RT in the same blocking solution containing 1:1000 dilutions of corresponding Alexa secondary antibodies (Invitrogen) made in goat and coupled to either 488 ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated in primary antibody diluted in blocking buffer using the following primary antibodies: anti-Phospho-Tau (Ser396) (1:500, 44-752G, Invitrogen), anti β-catenin (E-5 ...
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bioRxiv - Cancer Biology 2022Quote: ... in blocking buffer overnight in 4°C and stained with secondary AlexaFluor633 donkey anti-goat antibodies (Invitrogen, #A21082; 1:200) for 1 hour at RT ...
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bioRxiv - Biophysics 2022Quote: ... the tissues were washed with blocking solution three times and incubated with the respective secondary antibody (polyclonal goat anti-mouse IgG1, 1:500, Invitrogen) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated in a biotinylated goat anti-rabbit secondary antibody in blocking solution (Invitrogen, cat # 31820, 1:500) and transferred in a detection reagent (Vectastain Elite ABC kit ...
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bioRxiv - Molecular Biology 2023Quote: ... and probed with an Alexa Fluor 488-conjugated goat-anti-rabbit secondary antibody diluted in blocking buffer (1:100, #A11008, Invitrogen) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... retinas were incubated on a rocking platform for 2 hours at room temperature (RT) in Claudio’s blocking buffer (CBB) consisting of 1% FBS (LTID 10500-064, Thermo Fisher), 3% BSA (MB04602 ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 μl of blocking buffer containing a 1:1000 dilution of goat anti-rabbit Alexa 488 (Thermo Fisher; catalog #R37116) was added to the well and samples were incubated overnight on a rocking incubator at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The slides were then rinsed 4 times with the washing buffer for 15 minutes and incubated with the secondary antibody at 1:500 dilution in blocking solution for 1h at room temperature (Rabbit 555, Invitrogen, #AB_162543 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated in a biotinylated goat anti-rabbit secondary antibody in blocking solution (Invitrogen, cat # 31820, 1:500) and transferred in a detection reagent (Vecstatin Elite ABC kit ...