Labshake search
Citations for Thermo Fisher :
251 - 300 of 5409 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... ERCC Spike-In Controls (Invitrogen) were added to RNA samples (input RNA was 100ng per sample ...
-
bioRxiv - Cancer Biology 2020Quote: ... ERCC Spike-In Mix (ThermoFisher) was used as an internal control ...
-
bioRxiv - Plant Biology 2020Quote: ... ArrayControl RNA Spikes (Applied Biosystems; www.thermofisher.com/applied/biosystems ...
-
bioRxiv - Molecular Biology 2021Quote: ... ERCC spike-in controls (ThermoFisher) were added to 500 ng of total RNA and ribominus selection was performed using the NEBNext® rRNA Depletion Kit ...
-
bioRxiv - Neuroscience 2022Quote: ... ERCC Spike-In Controls (Invitrogen) were added to 100ng of RNA per sample prior to processing with the NEBNext Poly(A ...
-
bioRxiv - Cell Biology 2020Quote: ... ERCC spike-in RNA (Ambion) was added to the lysis buffer in a dilution of 1:4,000,000 ...
-
bioRxiv - Immunology 2022Quote: ... desalted (Zeba Spike Desalting, ThermoFisher), and loaded at a concentration of 500 nM onto streptavidin (SA ...
-
bioRxiv - Microbiology 2022Quote: ... ERCC spike mix 2 (Ambion) was added to the RNA ...
-
bioRxiv - Microbiology 2023Quote: ... ERCC spike-in controls (ThermoFisher) were added to 500-1000 ng of total RNA ...
-
bioRxiv - Developmental Biology 2023Quote: ... RNA spike-ins (Invitrogen, 4456740) were added to each purified RNA sample following the manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2024Quote: ... ERCC spikes (Thermo Fisher Scientific) were added to samples to control all the procedure ...
-
bioRxiv - Microbiology 2022Quote: ... S1 nuclease (Thermo Fisher Scientific, USA), and the restriction enzyme Hind III (New England BioLabs ...
-
bioRxiv - Cancer Biology 2022Quote: ... Ki67 (Fisher Scientific, RM-9106-S1), cleaved caspase 3 (Cell Signaling Technology ...
-
bioRxiv - Microbiology 2022Quote: ... 10 U S1 nuclease (Thermo Scientific), or 0.4 U RNase III (New England Biolabs ...
-
bioRxiv - Microbiology 2022Quote: ... 10 U S1 nuclease (Thermo Scientific) at 37°C for 1 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... S1 nuclease (200 U; Thermo Scientific) was added and incubated at 37 °C for 1 hour.
-
bioRxiv - Molecular Biology 2024Quote: ... 100U of S1 Nuclease (ThermoFisher Scientific) in 270 ul of ice-cold S1 reaction buffer (30mM Na actetate ...
-
bioRxiv - Biochemistry 2020Quote: ... Anti-His (C-term)-HRP (#46-0707, 1:2000 for dot blot, Invitrogen); Aβ (6E10 ...
-
bioRxiv - Genomics 2019Quote: ... An ArrayControl RNA Spots and Spikes kit (with spike numbers 1, 4 and 7) (Ambion) were used to monitor technical variability ...
-
bioRxiv - Neuroscience 2021Quote: ... and subjected to polyadenylating reaction using Escherichia coli poly(A) polymerase (E-PAP) Poly(A) Tailing Kit (Thermo Fisher Scientific, AM1350) in buffer containing 0.1mM ATP ...
-
bioRxiv - Biochemistry 2022Quote: ... The constructs were transformed into competent DH5α (E. coli) cells and plasmids were extracted using GeneJET plasmid miniprep kit (Thermo Scientific) to verify sequence integrity by sequencing (Eurofins Genomics) ...
-
bioRxiv - Cell Biology 2022Quote: ... worms were then transferred to 10 cm NGM conditioning plates (seeded with OP50 E. coli bacteria and with 12 μl 10% 2-butanone (Acros Organics) in ethanol streaked across the lid with a pipette tip for 1 h ...
-
bioRxiv - Microbiology 2023Quote: 50ng of each plasmid containing viral cDNA genome were used for transformation of competent bacteria (E. coli strain DH5α, Thermo Scientific) by heat-shock ...
-
bioRxiv - Neuroscience 2023Quote: ... The template was degraded by DpnI digest and circular PCR product was transformed into OneShot TOP10 chemically competent E coli (Invitrogen C4040). Both CMV:dreammist- GFPpA and CMV:dreammistA22W-GFPpA constructs were checked by Sanger sequencing.
-
bioRxiv - Genetics 2023Quote: ... worms were then transferred to 10 cm NGM conditioning plates (seeded with OP50 E. coli bacteria and with 18 mL 10% 2-butanone (Acros Organics) dissolved in ethanol on the lid ...
-
bioRxiv - Cell Biology 2023Quote: ... strain was maintained on standard nematode growth media (NGM) plates seeded with OP50 E. coli bacteria (at 20°C. LysoSensor Green DND-189 (Thermo Fisher) was diluted in M9 buffer and added to 6 cm standard NGM plates (containing 12 mL of agar ...
-
bioRxiv - Neuroscience 2022Quote: ... One-step real-time RT-PCR assays targeting SARS-CoV-2 nucleocapsid (N) coding region and subgenomic RNA were run on ABI 7900HT Sequence Detection Systems (Thermo Fisher Scientific), as previously reported25.
-
bioRxiv - Cancer Biology 2019Quote: ... then incubated with S1 nuclease buffer with or without 20 U/mL S1 nuclease (Invitrogen, 18001-016) for 30 min at 37 C ...
-
bioRxiv - Molecular Biology 2024Quote: ... then incubated with S1 nuclease buffer with or without 20 U/mL S1 nuclease (Invitrogen, 18001-016) for 30 min at 37 °C ...
-
bioRxiv - Cell Biology 2022Quote: Mosaic labelling of neuroepithelial cells was achieved with BioTracker™ 490 Green Cytoplasmic Membrane Dye (Thermo Fisher Scientific). Fixed cells were stained according to the manufacturer’s instructions for 30 minutes at room temperature.
-
bioRxiv - Microbiology 2022Quote: The Spike gene sequence from the severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1 (NC_045512.2) was codon optimized (GeneArt, ThermoFisher) and gene blocks with overlapping sequences were synthesized by Bio Basic Inc (Markham ...
-
bioRxiv - Immunology 2023Quote: ... and coronavirus antigens were expressed in Expi293F cells by transient transfection in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... followed by incubation in S1 nuclease buffer with or without 20 U/mL S1 nuclease (Thermo Fisher, 18001016) for 15 min at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... we designed construct PolI-SARS-CoV2-NLuc-N-HDR and ordered this as insert in the pMK-RQ backbone (Invitrogen construct ID 20ACX40P). The resulting plasmid pPolI-SARS-CoV2-NLuc-N was digested with XbaI prior to transfection to eliminate background (read-through ...
-
bioRxiv - Microbiology 2022Quote: SARS-CoV-2 N and goblet cells were co-stained using rabbit anti-SARS-CoV-2 Nucleocapsid monoclonal antibody (HL453) (Invitrogen, cat# MA5-36272) (1:100 dilution ...
-
bioRxiv - Microbiology 2021Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS-CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described [41] using the following primers and probes ...
-
bioRxiv - Immunology 2023Quote: Open-reading frames coding for either Nefmut alone or fused with SARS-CoV-2 N protein were cloned into pVAX1 plasmid (Thermo Fisher, Waltham, MA) as previously described [20] ...
-
bioRxiv - Microbiology 2023Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS- CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described (73 ...
-
bioRxiv - Immunology 2022Quote: Preparation of CD5L-coated pHrodo particles: one vial containing 2 mg of particles (pHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis, ThermoFisher) were reconstituted with 500 μL of TBS with 5 mM CaCL2 and sonicated for 5 min ...
-
bioRxiv - Biochemistry 2023Quote: ... Digested scFv inserts were subcloned in pET22b(+) vector (EMD Biosciences) and transformed into BL21(DE3) E-coli strain (Thermo Fisher Scientific, USA). Amber stop codon in CDR regions of scFvs were mutated to glutamine by site-directed mutagenesis kit (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... Primary antibodies of anti-SARS/SARS-CoV-2 Nucleocapsid Monoclonal Antibody (Invitrogen, MA1-7404) is diluted in section staining buffer at 1:400 and sections are stained at 4°C overnight ...
-
bioRxiv - Immunology 2021Quote: ... and Spike-Alexa488 (Thermofisher Scientific, S32354) and Spike-APC (Thermofisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... and Spike-APC (Thermofisher Scientific, S32362) or RBD-Alexa488 and RBD-APC ...
-
bioRxiv - Developmental Biology 2021Quote: ... and ERCC spike-in RNA (Ambion) (1:1,000,000 dilution ...
-
bioRxiv - Plant Biology 2021Quote: ... root and spike using Trizol (Invitrogen) method and Xcelgen plant RNA kit ...
-
bioRxiv - Cell Biology 2022Quote: ... ERCC RNA spike-in (Thermo Scientific) were diluted into the RT mix (dilution 5×10-7) ...
-
bioRxiv - Molecular Biology 2022Quote: ... ERCC RNA spike-in mix (Invitrogen) was added after shearing ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2µl of ERCC RNA spikes (ThermoFisher) were added to the eluate at a dilution of 1/25 000 for PFP and 1/5000 for PRP ...
-
bioRxiv - Genomics 2019Quote: ... and ERCC spike-in control (ThermoFisher). Plates were centrifuged and placed on dry ice immediately before storage at −80°C.
-
bioRxiv - Genomics 2021Quote: ... ERCC Spike-In (Thermofisher Scientific, 4456739) controls (0.5μl of mix 1 or mix 2 from 1:100 dilution ...