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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 cells were used as host cells to transfect the SARS-CoV-2 FSE dual luciferase reporter plasmid using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific). The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were probed with rabbit-anti-SARS spike (Invitrogen, PA1-411-1165, 0.5ug/ml), rabbit-anti-Orf6 (Abnova ...
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bioRxiv - Microbiology 2023Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: The Drosophila Schneider 2 (S2) cells (Invitrogen) were co-transfected with the EGFP-Caspase 2 or EGFP-Δ Caspase 2 plasmid and the synthesized miR-2 (miR-2-mimic ...
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bioRxiv - Biochemistry 2023Quote: ... Drosophila melanogaster S2 (Schneider 2) cells (Invitrogen), originally derived from a primary culture of late stage (20-24 h old ...
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bioRxiv - Microbiology 2021Quote: ... pCG1-SARS-2-SdC19 or pCG1-SARS-2-SdC19-H2 using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... containing 2% FBS and 1x Antibiotic-Antimycotic (AA, Gibco) (FACS buffer) ...
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bioRxiv - Biochemistry 2021Quote: ... His (1:1000, Invitrogen, mouse monoclonal), Flag M2(1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Recombinant coronavirus spike glycoproteins S (Table S1) were coated overnight onto NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) at 50 ng/well in 100 µL of PBS ...
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bioRxiv - Biophysics 2020Quote: CLGltPh and double cysteine mutants were transformed into Escherichia coli (E. coli) Top10 cells (Invitrogen). Bacterial cells were inoculated in Luria Broth medium containing 100 μg/mL ampicillin at 37 °C until OD600 ~0.6-0.8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from mock and SARS-CoV-2 infected proximal airway ALI and alveolar organoid cultures lysed in Trizol (Thermo Fisher Scientific) using the chloroform-iso-propanol-ethanol method ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Microbiology 2020Quote: The melting profiles were obtained incubating 10 ng of SARS-CoV-2 RNA with 10 or 100nM of DCV and Sybergreen (1x) (Thermo Fisher Scientific) in an StepOne™ Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Bioengineering 2021Quote: SARS-CoV-2 S was chemically biotinylated using EZ-Link™ NHS-LC-LC-Biotin following manufacturer instructions (Thermo Fisher, Cat#21343). Vero E6 cells (ATCC ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 isolate Wuhan-Hu-1 (GenBank accession #: MN908947) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher Scientific). After 48 to 72 hours ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Microbiology 2022Quote: ... The viral genome between 27,895-29,534 nucleotides based on the SARS-CoV-2 WA-1 strain was RT-PCR amplified using Super Script II Reverse transcriptase (Thermo Fisher Scientific) and Expanded High Fidelity PCR system (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: mRNAs were extracted from neurons derived from AD or Control hiPSCs with or without SARS-CoV-2 infection in Trizol reagent (Thermofisher, Waltham, MA) and followed by cDNA synthesis with SuperScript™ III Reverse Transcriptase kit (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... 10,000 Hela-hACE2 cells generated by lentivirus transduction of wild-type Hela cells and enriched by fluorescence-activated cell sorting (FACS) using biotinylated SARS-CoV-2 RBD conjugated with streptavidin-Alexa Fluor 647 (ThermoFisher Scientific, S32357) were added to the mixture with 20 µg/ml Dextran (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were infected with 0.1 MOI of SARS-CoV-2 using Opti-MEM I reduced serum medium (Thermo Fisher, Cat#31985062) and incubated for 1 hour at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR amplification of the viral genome spanning nucleotides 27,895-29,534 (according to the SARS-CoV-2 USA-WA1/2020 viral genome) was performed using Super Script II Reverse transcriptase (Thermo Fisher Scientific) and Expanded High Fidelity PCR System (Sigma Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... half the wells exposed to particulate and half the control wells were apically infected with SARS-CoV-2 derived from clinical isolate WA1 (20) in high glucose DMEM (Gibco 11995-065) with 5% heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... 11 cDNA fragments with 70 bp end-terminal overlaps which spanned the entire SARS-CoV-2 isolate Wuhan-Hu-1 genome (GenBank accession: NC_045512) were produced by GeneArt™ synthesis (Invitrogen™, ThermoFisher) as inserts in sequence verified ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with mouse mAb SARS-CoV-2 nucleocapsid antibody (SinoBiological, 1:100) and rabbit Claudin 7 polyclonal antibody (ThermoFisher, 1:200) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Genomics 2021Quote: PCR amplification of SARS-CoV-2 genome was carried out using AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, California, USA) with reaction constituents as 1X 360 master mix ...
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bioRxiv - Neuroscience 2022Quote: ... One-step real-time RT-PCR assays targeting SARS-CoV-2 nucleocapsid (N) coding region and subgenomic RNA were run on ABI 7900HT Sequence Detection Systems (Thermo Fisher Scientific), as previously reported25.
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bioRxiv - Immunology 2022Quote: ... Batches were sequenced using the Ion AmpliSeq SARS-CoV-2 Insight Research Assay on an Ion Torrent Genexus Integrated Sequencer (Thermo Fisher Scientific). Each run completed within 24h ...
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bioRxiv - Immunology 2023Quote: ... Extraction of SARS-CoV-2 RNA was performed using the MagMax Viral/Pathogen Nucleic Acid Isolation kit (Thermo Fisher Scientific, Waltham, MA) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... the transfected cells were harvested and stained with Alexa Fluor 647-anti-SARS-CoV-2 RBD antibody (51-6490-82; Thermo Fisher Scientific). Ancestral and variant spike expressing HEK293T cells were sorted for as target cells using the FACSAria™ Fusion Cell Sorter (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... the VSV antigenome without the G gene and the SARS-CoV-2 S gene were amplified by PCR using Platinum™ SuperFi II PCR Master Mix (Thermo Fisher). PCR products were gel-purified and assembled using NEBuilder® HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Immunology 2023Quote: ... For hamster samples a SARS-CoV-2 Nucleocapsid antibody (SinoBiological #40588) followed by a goat anti-rabbit IgG Alexa Fluor 488 antibody (ThermoFisher Scientific #A32731) was used (6) ...
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bioRxiv - Immunology 2023Quote: ... Results of the human samples were developed using a SARS-CoV-2 convalescent plasma as primary and a goat anti-human IgG Alexa Fluor 488 secondary antibody (ThermoFisher Scientific #A48276) (2) ...
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bioRxiv - Bioengineering 2024Quote: We conducted quality control checks of bead quality prior to cartridge loading with synthetic template standards of SARS-CoV-2 RNA (TWIST #102024) diluted in UltraPure™ DNase/RNase-free distilled water (UPdH2O - Invitrogen #10977015). To evaluate the performance of the NABIT reaction system ...
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bioRxiv - Microbiology 2024Quote: ... were transfected with the plasmid encoding the stabilized SARS-CoV-2 soluble S protein with the Expifectamine kit (GIBCO, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Codon-optimized gene encoding the full-length SARS-CoV-2 PLpro (1-315 amino acids) was custom synthesized (Invitrogen, Thermo Fisher Scientific) and was cloned using XhoI and NdeI restriction sites in pET28c vector containing an N-terminal 6X histidine tag ...
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bioRxiv - Molecular Biology 2023Quote: ... Codon-optimized gene encoding the full-length SARS-CoV-2 PLpro (1-315 amino acids) was custom synthesized (Invitrogen, Thermo Fisher Scientific) and was cloned using XhoI and NdeI restriction sites in pET28c vector containing an N-terminal 6X histidine tag ...
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bioRxiv - Microbiology 2021Quote: ... 5x-His mouse anti-tag was used at 1:500 (Invitrogen). Anti-mouse HRP secondary antibody was used at 1:10,000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... the following antibodies were used: 6x-His Tag monoclonal antibody (ThermoFisher, cat ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL of His-Tag magnetic beads (Dynabeads™ Invitrogen #10103D) was added to each tube ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken for immunoblotting using anti His-tag antibody (ThermoFisher). Crosslinking and control reactions intended for scramblase activity assays were stopped with 1 M Tris pH 7 to a final concentration of 10 mM ...
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bioRxiv - Biophysics 2023Quote: ... To cut the His-tag HRV 3C protease (Thermo-Fisher Scientific) was added to a molar ratio of 1:800 (enzyme ...