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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: RNA extraction of SARS-CoV-2 infected A549-ACE2 cells was performed by TRIzol (Thermo Fisher Scientific, 15596-026) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 infected VERO cells were lysed by adding 0.3-0.4 mL of TRIzol Reagent (Invitrogen, ThermoFisher Scientific) per 1 × 105-107 cells directly in the culture dish and pipetting up and down several times to homogenize ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 infected VERO cells were lysed by adding 0.3-0.4 mL of TRIzol Reagent (Invitrogen, ThermoFisher Scientific) per 1 × 105-107 cells directly in the culture dish and pipetting up and down several times to homogenize ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S protein Omicron genes were synthesized and inserted into pcDNA3.1 (GeneArt Gene Synthesis, Thermo Fisher Scientific). Pseudotyped retroviral particles were produced in HEK293T cells as described previously (32) ...
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bioRxiv - Microbiology 2021Quote: ... and 0.06 μg/ml or vehicle and 2 h later infected with SARS-CoV-2 (multiplicity of infection (MOI) = 0.01 and 0.1) in serum-free DMEM (Thermo Fisher) for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
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bioRxiv - Microbiology 2021Quote: ... Taqman gene expression assays for N2 and N3 regions of the SARS-CoV-2 N gene were made (ThermoFisher) following CDC specifications (Division of Viral Diseases ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 RBD (residues Arg319 to Lys529) was expressed by using the Bac-to-Bac Baculovirus System (Invitrogen). RBD containing the gp67 signal peptide and a C-terminal 6×His tag was inserted into pFastBac1 to form the plasmid pFastBac1-RBD ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated-SARS-CoV-2 tri-S proteins were mixed with FITC-labelled NeutrAvidin beads (1 µm, Thermo Fisher Scientific) (1 µg of tri-S for 1 µl of beads) ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-2P trimer was biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-APC (Agilent ...
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bioRxiv - Immunology 2021Quote: ... SARS-COV-2.SctΔ19 Wuhan plasmid was amplified by PCR with Phusion DNA polymerase (Thermo Scientific, ref F-549S) and the following primers ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transiently transfected with 2μg of either wild-type or mutant SARS-CoV-2 S protein plasmid with Lipofectamine 3000 (Invitrogen) at a ratio of 1:2:2 DNA ...
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Immunology 2021Quote: ... HEK293T and A549 cells were transfected with GFP-Flag or SARS-CoV-2 S-Flag (1.5 μg/ml) constructs using Lipofectamine 3000 reagent (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to Streptavidin-PE (Thermo Fisher). Streptavidin-BV510 (BD ...
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bioRxiv - Immunology 2021Quote: ... HEK293T and A549 cells were transfected with GFP-Flag (VB200507-2985cmv) or SARS-CoV-2 S-Flag (VB200507-2984jyv) (1.5 μg/ml) constructs using Lipofectamine 3000 reagent (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... SARS-CoV-2 detection was performed by quantitative real time PCR using Taqpath COVID-19 combo kit (Applied Biosystems, ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Molecular detection of SARS-CoV-2 was performed with TaqMan™ Gene Expression Assay (Cat. N. 4331182, ThermoFisher®) with specific primers/probes previously described40 ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μL of capture Ab (~4 μg/ml) or SARS-CoV-2 recombinant antigen (~0.2 mg/ml) in 1x PBS (Gibco) was loaded in each channel ...
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bioRxiv - Microbiology 2022Quote: ... COS-7 cells grown on 13 mm cover slips were transfected with either the empty pcDNA3.1(+) vector or one expressing the SARS-CoV-2 E protein using Turbofect transfection reagent (ThermoFisher). At 24 h pt ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then FISH-stained against SARS-CoV-2 ORF1a mRNA using ViewRNA mRNA FISH assay according to the manufacturer’s instructions (ThermoFisher) with some modifications ...
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bioRxiv - Microbiology 2022Quote: ... Full length genomic SARS-CoV-2 RNA was in vitro transcribed using mMESSAGE mMACHINE T7 Ultra Transcription kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and cloned using SacI and ApaI in the pCR3 SARS-CoV-2-SΔ19 expression plasmid (13) using Gibson Assembly (ThermoFisher). All constructs were verified by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Full length genomic SARS-CoV-2 RNA was in vitro transcribed using mMESSAGE mMACHINE T7 Ultra Transcription kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 3’ end RNA was synthesized by in vitro transcription using an mMESSAGE mMACHINE T7 kit (Invitrogen) with a DNA template (5’- TAATACGACTCACTATAGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAAUGACGTAGCATGCTACGCG -3’ ...
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bioRxiv - Microbiology 2023Quote: ... and the insect cell codon-optimized RBD at residues 330 to 521 of SARS-CoV-2 S protein (GenBank accession no. MN908947.3) was cloned into a pFastBac plasmid (Invitrogen). For the RBD-LTA fusion protein expression ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Pathology 2021Quote: ... blots were incubated with an anti-influenza A NP protein antibody 19C10 generated by our laboratory (1:1000) and anti-V5 tag antibody (Thermo,1:5000) and visualized with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Invitrogen, 1:5000).
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... Goat anti-mouse IgG horseradish peroxidase-conjugated secondary antibody and goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody purchased from Thermo Fisher Scientific (USA).
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bioRxiv - Microbiology 2021Quote: ... 5 × 106 HEK 293T cells were co-transfected with pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 Spike (D614G) plasmid or its derivatives (B.1.1.7 and B.1.617) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Supernatants containing pseudotyped viruses were harvested 48 h post-transfection ...
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bioRxiv - Immunology 2022Quote: ... Cells were then blocked with 20% FBS in PBS and incubated with a rabbit polyclonal antibody that recognizes SARS-CoV-2 spike RBD (40592-T62, SinoBiological) followed by an Alexa Fluor 594-conjugated goat anti-rabbit IgG (A11037, Invitrogen). DNA was stained with Hoechst ...
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bioRxiv - Microbiology 2021Quote: ... cells in each well were infected with 2000 TCID50 SARS-CoV-2 pseudovirus in 100 µL of culture medium containing 2% FBS (Gibco, Cat. No. 10099141C). Culture medium was refreshed every 12 hours ...
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bioRxiv - Microbiology 2022Quote: ... ERCC spike mix 2 (Ambion) was added to the RNA ...
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV RBD (residues Arg306-Phe527) were inserted into pFastBac (Invitrogen) with an N-terminal gp67 signal peptide and a C-terminal 6 × His tag ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Drosophila Schneider 2 (S2) cells (Thermofisher) were maintained in Schneider’s Drosophila Medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV2 spike and RBD were conjugated with Alex647 fluorescence (Thermo Fisher A30009).
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bioRxiv - Microbiology 2020Quote: ... IAV (H1N1 PR8 strain) and SARS-CoV-2 were grown in Vero cells with VP-SFM medium (11681-020, Gibco). DENV ...
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bioRxiv - Immunology 2021Quote: ... the synthetic DNA encoding the RBD of SARS-CoV-2 was synthesized by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA) and used as template for the amplification of the three RBM epitopes (Table 2) ...
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bioRxiv - Molecular Biology 2020Quote: ... Detection of SARS-CoV-2 N1 gene sequences and host RNase P mRNA from de-identified SARS-CoV-2 positive samples was carried out with the one-step TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher), using 2 μL of undiluted PEARL extract ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 S.dCT were co-transfected into HEK293T cells using lipofectamine 2000 (Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Bioengineering 2021Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S and RBD proteins were transiently expressed in Freestyle 293F system (ThermoFisher). In brief ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... extracted DMS-modified RNA from SARS-CoV-2 infected Vero cells was fragmented using the RNA Fragmentation kit (ThermoFisher Scientific). 1.5 μg of rRNA subtracted total RNA was fragmented at 70°C for 2.5 min ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 106 HEK 293T cells were co-transfected with 6 μg each of pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 S plasmids (pS-D614 or pS-G614) using Lipofectamine 3000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 SΔCT were co-transfected into HEK293T cells by lipofectamine 2000 (Thermo Fisher Scientific). The supernatants containing the pseudotype viruses were collected 48 h after transfection ...