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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 Inhibitor Screening Kit (Fisher Scientific GmbH, Schwerte, Germany) was used according to the manufacturer’s instructions and as described previously [12].
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies against SARS-CoV-2 nucleocapsid (PA1-41098, Invitrogen™), SARS-CoV-2 spike protein (S1 ...
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bioRxiv - Microbiology 2020Quote: ... codon-optimized (for human cells) SARS-CoV-2 DNA (GeneArt Gene Synthesis, Thermo Fisher Scientific) based on the publicly available protein sequence in the National Center for Biotechnology Information database (NCBI Reference Sequence ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained with mouse anti-SARS-CoV-2 N (#MA5-29981; Thermo Fisher Scientific) and anti-mouse IgG Alexa488 (#A-11029 ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 RBD 6xHisTag constructs were expressed by transient transfection of Expi293F cells (Gibco) and purified using HisTrap FF and HisTrap HP columns (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 pseudoparticles were produced by co-transfecting HEK293T cells using Lipofectamine 2000 (Invitrogen) with plasmids encoding HIV-1 gag/pol ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 infected cells were washed with PBS and lysed with TRIzol reagent (Invitrogen). Total RNA was extracted using RNeasy mini kit (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... pylori NAP proteins (SARS-CoV-2S6p3 and SARS-CoV-2S6p312, respectively) were produced upon transient expression in Expi293F cells (ThermoFisher). Clarified supernatants were purified by affinity chromatography using an anti-HA affinity matrix (Cat# 11 815 016 001 ...
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bioRxiv - Biochemistry 2022Quote: ... hPolεCD(insect) with a cleavable N-terminal His-Tev tag was cloned into pFastBac-1 plasmid (Invitrogen). hPolεCD and hPolεCD(exo- ...
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bioRxiv - Biochemistry 2021Quote: The expression plasmid encoding for codon-optimized SARS-CoV-2 RBD (329-538) with a C-terminal hexa-histidine tag was transfected into Expi293™ GnTI-(Thermo Fisher) cells (1×106 cells/mL ...
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bioRxiv - Microbiology 2022Quote: ... the expression plasmid encoding for codon-optimized SARS-CoV-2 RBD (329-538) with a C-terminal hexa-histidine tag was transfected into Expi293™ GnTI- (Thermo Fisher) cells (1×106 cells/mL ...
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bioRxiv - Immunology 2021Quote: ... biotinylated SARS-CoV-1 or SARS-CoV-2 prefusion-stabilized S trimer was incubated with red streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2 h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Blots were probed with primary antibodies: human anti-SARS-CoV-2 Spike (S-ECD/RBD) (cat# bcb03, Thermo Fisher, 1:1000, non-reducing conditions), anti-Salmonella typhimurium LPS (cat# ab8274 ...
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bioRxiv - Immunology 2022Quote: ... and the QI9 peptide (QYIKWPWYI, residues 1208-1216 of the SARS-CoV-2 spike protein) maintained in RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS and 30 U/ml recombinant human IL-2 (Peprotec ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted from HEK293T cell line 30 hours post transfection (HA-NRP1 and SARS-CoV-2-S1493-685 and or HA-NRP1-4Cys-4Ala and SARS-CoV-2-S1493-685) using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Viral RNA from SARS-CoV-2 infected HBEC cell lysates was extracted using TRIzol (Life Technologies) and purified using Direct-zol RNA MiniPrep Plus according to manufacturer’s instructions (Zymo Research ...
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bioRxiv - Immunology 2020Quote: ... and a TwinStrepTag (SARS-CoV-2 HexaPro S) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Cell Biology 2021Quote: ... and Jurkat cells with stable SARS-CoV-2 S expression were labeled with CFSE dye (ThermoFisher). 75,000 Jurkat cells were added to macrophage cells in a 96-well plate in the presence of 2G1 or the isotype control antibody ...
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bioRxiv - Genomics 2021Quote: Total RNA from SARS-CoV-2 infected cell samples was extracted with Trizol reagent (Invitrogen, 15596026), and then subjected to the rRNA depletion with the Ribo-off rRNA Depletion Kit (Human/Mouse/Rat ...
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bioRxiv - Bioengineering 2021Quote: ... Freshly-thawed SARS-CoV-2 was diluted in infection media (DMEM (Gibco) supplemented with 0.1% bovine serum albumin (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... SARS-Cov-2 ORF1b FAM assay (PN 4332079, Life Technologies assay# APGZJKF) or spike (S ...
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bioRxiv - Microbiology 2021Quote: ... anti-SARS-CoV-2 (PA1-41098, Invitrogen, Thermo Fisher Scientific, Massachusetts, USA), anti-PEDV (9191 ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SARS-CoV-2 RBD were purified by nickel affinity columns (Invitrogen) while ACE2-Fc and antibodies were purified by protein A affinity columns (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-SARS-CoV-2 S antibody (PA5-81795, Invitrogen, 1:1000); rabbit anti-TDRD3 antibody (Bethyl Laboratory ...
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bioRxiv - Microbiology 2020Quote: ... anti-SARS CoV-2 nucleocapsid mouse monoclonal (1:10) (Thermo Fisher Scientific) and anti-ACE2 mouse monoclonal (1:20 ...
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bioRxiv - Immunology 2022Quote: ... and CCR5: rabbit anti-SARS-CoV-2 S1 protein (PA5-81795; ThermoFisher) at 1:2,500 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The MERS-CoV viral lysate was immuno-detected with a MERS-coronavirus spike protein S2 polyclonal antibody (Invitrogen, Carlsbad, CA) followed by a donkey anti-rabbit IgG (H+L) ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Neuroscience 2023Quote: ... twice rinsed in S2 media (Schneider’s Insect Medium, Fisher Scientific), and dissected in cold S2 media on a 9-well glass plate coated with silicone (Sylgard 184 ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 isolates were propagated in Vero E6 cells in Opti-MEM I (Invitrogen, Cat. # 51985091) containing 0.3% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: ... ChAdOx1 SARS CoV-2 S viral vectors were rescued in T-REx™ cells (Invitrogen, Cat. R71007), a derivative of HEK293 cells which constitutively express the Tet repressor protein and prevent antigen expression during virus production ...
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bioRxiv - Biochemistry 2021Quote: The SARS-CoV-2 S ectodomains were produced in 200 mL cultures of Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Milling of SARS-Cov-2 infected cells was carried out using a Scios DualBeam cryoFIB (ThermoFisher Scientific) equipped with a PP3010T transfer system and stage (Quorum Technologies) ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 S protein-expressing plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen, USA). 24 h after transfection ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 isolates were propagated in VeroE6 cells in Opti-MEM I (Invitrogen, Carlsbad, CA, USA) containing 0.3% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 S ectodomains were produced in 100 mL cultures of Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... or N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher) as previously described [18] ...
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bioRxiv - Microbiology 2020Quote: cDNA of SARS-CoV-2 was made using SuperScript IV Reverse Transcriptase (Invitrogen) following manufacturer’s instructions with modifications ...
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bioRxiv - Biochemistry 2021Quote: ... and 1:4 pD614G-SARS-CoV-2-spike:pPyEGFP using Lipofectamine 2000 (Life Technologies), and media was replaced on day 1 ...
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bioRxiv - Immunology 2020Quote: ... and N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher), i.e. ...
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bioRxiv - Immunology 2021Quote: ... Libraries were prepared using Ion AmpliSeq SARS-CoV-2 Kit (Thermo Scientific, USA) following the manufacturer’s Protocol ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 S genes using Lipofectamine 3000 transfection reagent (ThermoFisher, CA)15 ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 E protein (1 to 75 amino acids) (ThermoFisher Scientific) in coating buffer [0.05 M sodium carbonate-sodium bicarbonate (pH 9.6)] at 4°C for overnight incubation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Drosophila Schneider 2 (S2) cells (Thermofisher) were maintained in Schneider’s Drosophila Medium (Gibco ...
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bioRxiv - Immunology 2021Quote: ... spike glycoproteins were produced in Expi293F cells grown in suspension using Expi293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... Drosophila S2 cells were grown to log phase in HY Q-SFX insect medium (ThermoFisher) supplemented with 18 mM L-Glutamine and harvested by scraping ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT were co-transfected into HEK293T cells (ATCC CRL_3216) with lipofectamine 2000 (ThermoFisher Scientific). Pseudoviruses of SARS-CoV-2 variants were generated by using WA1/2020 strain (Wuhan/WIV04/2019 ...