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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... and 0.06 μg of CMV-promoter driven plasmid encoding SARS-CoV-2 wild type or mutant spike variants using Lipofectamine™ 2000 transfection reagent (ThermoFisher). Pseudovirus was harvested by collecting supernatant 48 h after transfection and filter sterilized (0.44 μm ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 SARS-CoV-2 spikeΔ19 plasmid (encoding SARS-CoV-2 spike protein, with 19 AA truncated in C terminal) using Lipofectamine 3000 (Invitrogen, L3000-015) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 5% FBS and 0.5% Triton X-100) for 30 minutes and washed before incubation with an anti-SARS-CoV-2 spike protein antibody (Life Technologies). Nuclei and F-actin were stained using DAPI and Phalloidin (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 virus was labelled using first an anti-SARS-CoV-2 antibody (Spike protein, Thermo Fischer) and then a secondary anti-rabbit Alexa 647 (Thermo Fisher).
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bioRxiv - Immunology 2021Quote: ... and the SARS-CoV-2 spike gene was reverse-transcribed and amplified with a SuperScript IV One-Step RT-PCR kit (ThermoFisher Scientific) using primers flanking the S gene ...
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bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
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bioRxiv - Immunology 2020Quote: ... The cells were blocked with 5 % skim milk-PBS for 1 hour at RT and incubated with an antibody against SARS-CoV-2 spike (BLSN-005P, Beta Lifescience) or control IgG (Thermo Fisher) at 4 °C overnight ...
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bioRxiv - Genomics 2023Quote: ... The trimeric RhGB07 spike protein was expressed as previously reported as for the SARS-CoV-2 spike transiently expressed in suspension-adapted ExpiCHO cells (Thermo Fisher) in ProCHO5 medium (Lonza ...
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used were: 1:1000 mouse anti-SARS/SARS-CoV-2 N (ThermoFisher Scientific; ...
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bioRxiv - Microbiology 2022Quote: ... and stained using SARS/SARS-CoV-2 Coronavirus Nucleocapsid Monoclonal Antibody (Invitrogen MA5-29981) as the primary antibody followed by Alexa647-conjugated secondary antibody (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies of anti-SARS/SARS-CoV-2 Nucleocapsid Monoclonal Antibody (Invitrogen, MA1-7404) is diluted in section staining buffer at 1:400 and sections are stained at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the spike protein was detected using anti-SARS-CoV Spike Protein (SDelta3) monoclonal antibody (7G12, Thermo Fisher Scientific, CA, USA)) raised against immunogen aa168-461 of the S1 domain.
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... and anti-SARS-CoV-2 S2 (Thermo Fisher # PA5-112048). The inability of the rescued virus to infect BHK-21 cells in the absence of hACE2 was assessed by fluorescence microscopy and flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... and anti-SARS-CoV-2 Nucleocapsid (Thermo Fisher; MA5-29982). HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were purchased from Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... HIV-based lentiviral pseudotyped particles harboring the SARS-CoV-2 Spike protein variants were produced using the ViraPower Lentiviral Expression system (Thermo Fisher Scientific) according to manufacturer’s protocol with minor changes ...
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bioRxiv - Immunology 2023Quote: ... The cells were then transfected with ancestral or variant SARS-CoV-2 spike protein encoding plasmid using Lipofectamine 3000 Transfection Reagent (L3000001; Thermo Fisher Scientific). After 24 hours ...
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bioRxiv - Bioengineering 2020Quote: ACE-Fc fusion proteins and the SARS-CoV-2 RBD were expressed in suspension-HEK293F cells grown in FreeStyle media (Gibco). Transfections were done using 40 kDa polyethyleneimine (PEI-MAX ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with a plasmid coding for SARS-CoV-2 RBD or ACE2-Fc and were purified on Ni-NTA resin (Invitrogen) or Protein A resin (Cytiva) ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (SouthernBiotech ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Molecular Biology 2021Quote: 2 × 20 μg of each of the three different SARS-CoV-2 spike proteins were mixed with 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and up to 10 mM dithiothreitol (DTT ...
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bioRxiv - Immunology 2022Quote: ... using the Ion AmpliSeq SARS-CoV-2 Research Panel (ThermoFisher Scientific). 8-16 barcoded samples were pooled and loaded on one Ion 530 chip using the Ion Chef Instrument (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... synthetic SARS-CoV-2 control (nCov19 control kit by Applied Biosystems) ranging from 5-0.1ng and 1-0.1ng concentrations respectively.
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bioRxiv - Microbiology 2021Quote: ... or SARS-CoV-2 E protein (1-75 aa) (Thermo Fisher) in coating buffer [0.05 M sodium carbonate–sodium bicarbonate (pH 9.6)] ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 infected-cells were suspended in PBS (Life Technologies) containing 1% FBS and 2mM EDTA (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 RBDs were expressed in Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 RBD was coated onto Immuno-MaxiSorb plates (Nunc) at final concentration of 2 μg/mL for 2 hr at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 Inhibitor Screening Kit (Fisher Scientific GmbH, Schwerte, Germany) was used according to the manufacturer’s instructions and as described previously [12].
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies against SARS-CoV-2 nucleocapsid (PA1-41098, Invitrogen™), SARS-CoV-2 spike protein (S1 ...
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bioRxiv - Microbiology 2021Quote: ... with C-terminal HRV3C-cleavable 8xHis tag) or SARS-CoV-2 RBD (residue 319-537) were transfected into Expi293F GnTI-Cells (Thermo Fisher Scientific) using PEI ...
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bioRxiv - Biochemistry 2021Quote: ... through G-25 resin the peptide-mRNA:cDNA libraries were incubated with cross-linked linked SARS-CoV-2 Mpro (200 nM) immobilized on Dynabeads™ His-tag Isolation & Pulldown (Life Technologies) at 4 °C for 30 min after which the beads were washed with ice-cold selection buffer (3 x 100 μL) ...
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bioRxiv - Biochemistry 2021Quote: The expression plasmid encoding for codon-optimized SARS-CoV-2 RBD (329-538) with a C-terminal hexa-histidine tag was transfected into Expi293™ GnTI-(Thermo Fisher) cells (1×106 cells/mL ...
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bioRxiv - Microbiology 2022Quote: ... the expression plasmid encoding for codon-optimized SARS-CoV-2 RBD (329-538) with a C-terminal hexa-histidine tag was transfected into Expi293™ GnTI- (Thermo Fisher) cells (1×106 cells/mL ...
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bioRxiv - Immunology 2021Quote: ... biotinylated SARS-CoV-1 or SARS-CoV-2 prefusion-stabilized S trimer was incubated with red streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2 h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Blots were probed with primary antibodies: human anti-SARS-CoV-2 Spike (S-ECD/RBD) (cat# bcb03, Thermo Fisher, 1:1000, non-reducing conditions), anti-Salmonella typhimurium LPS (cat# ab8274 ...
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bioRxiv - Immunology 2022Quote: ... and the QI9 peptide (QYIKWPWYI, residues 1208-1216 of the SARS-CoV-2 spike protein) maintained in RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS and 30 U/ml recombinant human IL-2 (Peprotec ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted from HEK293T cell line 30 hours post transfection (HA-NRP1 and SARS-CoV-2-S1493-685 and or HA-NRP1-4Cys-4Ala and SARS-CoV-2-S1493-685) using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with 2 µg pcDNA3.1 CoV-2 Spike using Lipofectamine2000 (Thermo Fisher). At 24 hours post-transfection ...
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bioRxiv - Bioengineering 2021Quote: ... Freshly-thawed SARS-CoV-2 was diluted in infection media (DMEM (Gibco) supplemented with 0.1% bovine serum albumin (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... SARS-Cov-2 ORF1b FAM assay (PN 4332079, Life Technologies assay# APGZJKF) or spike (S ...
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bioRxiv - Microbiology 2021Quote: ... anti-SARS-CoV-2 (PA1-41098, Invitrogen, Thermo Fisher Scientific, Massachusetts, USA), anti-PEDV (9191 ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SARS-CoV-2 RBD were purified by nickel affinity columns (Invitrogen) while ACE2-Fc and antibodies were purified by protein A affinity columns (Cytiva ...