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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S RBD-serum mixtures were loaded onto MaxiSorp 96F plates (Nunc) coated with 200 ng/well ACE2 [produced in-house as described in Bosnjak et al.(25)] and blocked with 2% bovine serum albumin/2% mouse serum (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S protein was conjugated to streptavidin-AF488 (Life Technologies #S11223), the HCoV-HKU1 S protein to streptavidin-BV421 (BD Pharmingen #563259 ...
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bioRxiv - Microbiology 2023Quote: ... The pBAC-SARS-CoV-2-mCherry-2A was digested with BstBI (FastDigest, Thermo Fisher) and BamHI (FastDigest ...
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bioRxiv - Bioengineering 2022Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S proteins were transiently expressed in Freestyle 293F system (ThermoFisher) whereas RBD proteins were expressed in the Expi293 system (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: RNA extraction of SARS-CoV-2 infected A549-ACE2 cells was performed by TRIzol (Thermo Fisher Scientific, 15596-026) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 infected VERO cells were lysed by adding 0.3-0.4 mL of TRIzol Reagent (Invitrogen, ThermoFisher Scientific) per 1 × 105-107 cells directly in the culture dish and pipetting up and down several times to homogenize ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 infected VERO cells were lysed by adding 0.3-0.4 mL of TRIzol Reagent (Invitrogen, ThermoFisher Scientific) per 1 × 105-107 cells directly in the culture dish and pipetting up and down several times to homogenize ...
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bioRxiv - Immunology 2020Quote: ... S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to Streptavidin-PE (Thermo Fisher). Streptavidin-BV510 (BD ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Biochemistry 2020Quote: ... and SARS-CoV-2 [3]) was performed with Proteome Discoverer version 2.4 (Thermo Fisher Scientific). The search was restricted to human and SARS-COV-2 proteins ...
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bioRxiv - Microbiology 2022Quote: ... human Anti-SARS COV-2 Nucleocapsid monoclonal (E16C) (Thermo Fisher, MA1-7403, 0.1 mg/mL), was incubated as a 1:100 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... to 1 µg/ml and mouse SARS-CoV-2 Nucleoprotein monoclonal antibody (MA1-7404, Thermofisher) to 10 µg/ml ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Microbiology 2020Quote: ... the purified SARS-CoV-2 RBD protein was coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at around 5μg/mL in PBS overnight ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Validation and SARS-CoV-2 genome detection was performed with TaqMan detection systems (Applied Biosystems) based qPCR using SARS-CoV-2 specific primers and probes with the following sequences ...
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bioRxiv - Microbiology 2021Quote: ... the purified SARS-CoV-2 RBD proteins were coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at 5μg/mL in PBS overnight ...
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bioRxiv - Immunology 2021Quote: ... RBD binding inhibition was measured using a SARS-CoV-2 Neutralizing Ab ELISA Kit (Invitrogen). For anti-Spike IgG experiments ...
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bioRxiv - Pathology 2022Quote: ... and either pMD2.G or pTwist-EF1alpha-SARS-CoV-2-S-2xStrep using LTX (Invitrogen) transfection reagents according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... indicated SARS-CoV-2 Z-RNAs) was incubated with Lipofectamine 2000 transfection reagent (Invitrogen, 11668019), for 15 mins at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of N501Y-SARS-CoV-2 BAC were transfected into Vero E6 (ATCC) with Lipofectamine 3000 (Invitrogen) in a 6-well plate according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Dynabeads His-Tag Isolation & Pulldown (Invitrogen) were washed in PBS-TH (PBS ...
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bioRxiv - Immunology 2021Quote: ... magnetic beads (Dynabeads His-Tag, Invitrogen) were coated with histidine tagged S protein according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) was coated at 2 μg/mL in PBS onto half-area 96-well high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) or streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2020Quote: ... Anti‐His tag monoclonal antibody (Invitrogen) was used in western blot analysis.
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bioRxiv - Microbiology 2021Quote: ... Binding studies in HEK 293T cells were performed using rVSV-SARS-CoV-2 Spike virions (rVSV/Spike) and binding was performed at room temperature in IMMULON 2HB flat bottom plates (Thermo Scientific, Waltham, MA).
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μl of Dynabeads™ His-Tag Isolation and Pulldown beads (ThermoFisher 10104D) for each CFPS reaction were equilibrated twice in equal volumes of wash buffer before addition to the purified reactions to remove the Ulp-protease and incubated with spinning at 4 °C for at least 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... IAV (H1N1 PR8 strain) and SARS-CoV-2 were grown in Vero cells with VP-SFM medium (11681-020, Gibco). DENV ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 S.dCT were co-transfected into HEK293T cells using lipofectamine 2000 (Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Bioengineering 2020Quote: ACE-Fc fusion proteins and the SARS-CoV-2 RBD were expressed in suspension-HEK293F cells grown in FreeStyle media (Gibco). Transfections were done using 40 kDa polyethyleneimine (PEI-MAX ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... extracted DMS-modified RNA from SARS-CoV-2 infected Vero cells was fragmented using the RNA Fragmentation kit (ThermoFisher Scientific). 1.5 μg of rRNA subtracted total RNA was fragmented at 70°C for 2.5 min ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 SΔCT were co-transfected into HEK293T cells by lipofectamine 2000 (Thermo Fisher Scientific). The supernatants containing the pseudotype viruses were collected 48 h after transfection ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA mini kit (Invitrogen) and subjected to cDNA synthesis using a PrimeScript RT reagent kit (Perfect Real Time ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 D614G S ectodomain was produced in Expi293F cells grown in suspension using Expi293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Microbiology 2021Quote: ... cells transfected with the SARS-CoV-2 S protein deletion mutants and controls were washed once with DPBS (Fisher Scientific), fixed with 4% (w/v ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA Mini Kit (Invitrogen). Thereafter ...
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bioRxiv - Immunology 2020Quote: ... Cryopreserved PBMC from the SARS-CoV-2-infected participant and a SARS-naïve donor were thawed at 37°C and stained for SARS-CoV-2-specific memory B cells with a flow cytometry panel consisting of: a viability dye (7AAD, Invitrogen), CD14 PE-Cy5 ...
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bioRxiv - Immunology 2022Quote: All SARS-CoV-2 S ectodomains were produced in 500-mL cultures of FreeStyle™ 293-F cells (ThermoFisher Scientific) grown in suspension using FreeStyle 293 expression medium (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: The SARS-CoV-2 pseudovirus-based neutralization assay were performed in Nunc 96-well cell culture plates (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
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bioRxiv - Biochemistry 2022Quote: ... RBD of SARS-CoV2 and SARS-CoV S were transiently expressed in FreeStyle™ 293-F cells (Thermo Fisher Scientific). Cells were resuspended at 1×106 per mL with fresh media on the day of transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... complementary cDNA of SARS-CoV-2 clinical samples was generated using SuperScript II Reverse Transcriptase (Invitrogen), random primers and 10μL of RNA ...
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bioRxiv - Bioengineering 2021Quote: Both Nefmut/SARS-CoV-2-based fusion proteins were cloned into the pVAX1 plasmid (Thermo Fisher) as already described (22) ...
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bioRxiv - Microbiology 2021Quote: ... The purified bacmid of SARS-CoV-2 was used for transfection using cellfectin (Thermo Fisher, SG). The viral stocks obtained from transfection were amplified and expressed for protein production ...
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bioRxiv - Cell Biology 2021Quote: ... employing the Ion AmpliSeq SARS-CoV-2 RNA custom primers panel (ID: 05280253, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: Artificial codon optimized genes encoding SARS-CoV-2 nsp10 and nsp16 proteins were commercially synthesized (Invitrogen) and cloned in a pSUMO vector that encodes an N-terminal His8x-SUMO tag ...
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bioRxiv - Immunology 2020Quote: ... We synthesized oligonucleotide fragments encoding the SARS-CoV-2 gene using GeneArt strings (Thermo Fisher Scientific) and assembled into the plasmid vector with NEB HiFi assembly (New England BioLabs) ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR detection of SARS-CoV-2 was performed on a QuantStudio 5 instrument (Applied Biosystems) using a TaqPath 1-step RT-qPCR Master Mix (Applied Biosystems ...