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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Rnase 3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... HEK293 cells were transfected using lipofectamine 2000 (Thermofisher, USA) following the manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were propagated in MEM with GlutaMAXTM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID: CVCL_6911) was purchased from Thermo Fisher Scientific (#R70007) ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
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bioRxiv - Neuroscience 2022Quote: ... For RNase-resistance assays 100 U RNase I (Thermo Fisher) was added per 100 μg total RNA in clarified lysates ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM MgCl2) supplemented with RNase inhibitor (RNase out, Invitrogen) and Proteinase inhibitor (Complete ...
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bioRxiv - Genetics 2023Quote: ... 80 units of RNAse-OUT RNAse inhibitor (Invitrogen, #15401-029), 0.2% Tween-20 (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 20 U/mL RNase inhibitor RNase Out (Invitrogen), 1 mM PMSF ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% HI FBS (Gibco A31604), and pen/strep and seeded into 96-well plates 24 hr prior to treatment ...
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bioRxiv - Neuroscience 2022Quote: ... and pcDNA3.1/myc-His (Invitrogen). Constructs for hTara mutants were prepared by cloning into pEGFP-C3 ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-FBS (Gibco, 16140071) + 10% HI-Horse Serum (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco). Neutrophils were recovered from the bottom of the tube.
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bioRxiv - Microbiology 2023Quote: ... GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E) ...
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bioRxiv - Molecular Biology 2023Quote: ... His-bind magnetic Dynabeads (Invitrogen) were washed with TBS containing 0.05% Tween-20 (TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco).
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bioRxiv - Molecular Biology 2023Quote: ... anti-His (ThermoFisher scientific, rd230540a), anti-SETD6 (Genetex ...
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bioRxiv - Cancer Biology 2023Quote: ... with 2% HI FBS (Gibco) and 0.4% 0.5M EDTA (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and 10% HI FBS (Gibco). Media were supplemented with interleukin-2 (IL-2 ...
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bioRxiv - Molecular Biology 2019Quote: ... RNase inhibitor (ThermoFisher). Protein concentrations were determined using BCA Protein assay Kit (Thermo Scientific Pierce) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNase I (Invitrogen) 50 U/mg of extract was used at 37 °C for 5 minutes ...
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bioRxiv - Immunology 2019Quote: ... RNase OUT (Invitrogen) and MMLV Reverse Transcriptase (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... RNase cocktail (Ambion) was also included in the lysis buffer at a concentration of 5 μl/ml to prevent non-specific interactions mediated by RNA ...
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bioRxiv - Microbiology 2020Quote: ... RNase A (Invitrogen) and 1X cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... RNase A (ThermoFisher), or RNA oligomers (Table 2 ...
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bioRxiv - Microbiology 2020Quote: ... RNase OUT (Invitrogen) and EDTA (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNase I (Ambion) was added to final concentration 0.5 U/µl (replicate 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... RNase H (Ambion) was added to remove the RNA strand of DNA-RNA duplexes ...
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bioRxiv - Pathology 2021Quote: ... RNase I (Ambion) was added to final concentration 2.7 ...
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bioRxiv - Neuroscience 2022Quote: ... RNAse block (Ambion), DIG RNA labelling mix (Roche Applied Science ...
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bioRxiv - Neuroscience 2020Quote: ... RNAse inhibitor (Invitrogen SUPERase-In RNase Inhibitor ...
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bioRxiv - Genomics 2021Quote: ... RNase A (Invitrogen, 1004D was added and the samples were incubated overnight at 65°C ...
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bioRxiv - Microbiology 2023Quote: ... RNase out (Invitrogen), and 1 mM DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... RNase I (Ambion) was added to the extract ...
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bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
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bioRxiv - Immunology 2020Quote: ... or equimolar mix of two human APOBEC3A siRNAs (Silencer 45715 and 45810, respectively, with sense sequences 5’-GACCUACCUGUGCUACGAATT-3’ and 5’-GCAGUAUGCUCCCGAUCAATT-3’, Life Technologies) using Lipofectamine RNAiMAX (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... or si-hsa-CHST15_0003 (n□=□3) or si-hsa-TNFRSF21_0001 (n□=□3) for 24□hours was carried out by using human Clariom™ Sassay (ThermoFisher Scientific) at the Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Cell Biology 2023Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... All collected fractions (3 ml) were analyzed using SDS-PAGE and Western blotting with an anti-His-tag antibody (ThermoFisher Scientific).
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Immunology 2023Quote: ... mammalian expression constructs carrying C-terminal 6X-His tagged gene of interest (HpARI1-3, or ST2 ectodomain) were transfected individually into Expi293F cells (Thermo Fisher) using the Expifectamine transfection kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Cell Biology 2020Quote: ... and ESRG_cn2 (Hs06675423_cn) detected the ESRG locus and TaqMan Copy Number Reference Assay human RNase P (4403326, Thermo Fisher Scientific) was used as an endogenous control ...
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bioRxiv - Systems Biology 2023Quote: 1000-3000 IEQ human islets pellets were resuspended in 200 µL PBS and treated with 20 µL 10 mg/mL Rnase A (Invitrogen) and 20 µL Protein Kinase K (Qiagen ...