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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Recombinant Zebrafish tnfa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Recombinant gH/gL proteins were biotinylated using the EZ-Link NHS-PEG4-Biotin Kit (ThermoFisher, catalog #21330) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Microbiology 2020Quote: The recombinant proteins were purified using nickel sepharose beads (Ni-NTA, Thermo Fisher Scientific, Waltham, MA, USA). The lysate supernatant from the 50 ml culture was mixed with 300 μl Ni-NTA beads and incubated overnight at 4° in the lysis buffer supplemented with 5 mM imidazole ...
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Pathology 2020Quote: ... A 1ug/mL mixture of 50μL per well containing recombinant protein in carbonate buffer (0.1M Na2CO3 0.1M NaHCO3 pH 9.3) was used to coat MaxiSorp (ThermoFisher) 96-well plates overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed and purified using a mammalian expression system via Expi293F cells (Life Technologies), as described in detail earlier (30 ...
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bioRxiv - Plant Biology 2022Quote: For recombinant protein expression in Pichia pastoris KM71H (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs encoding SCPSs ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant Streptococcus pyogenes Cas9 protein (SpCas9; Synthego) using Lipofectamine CRISPRMAX Cas9 Transfection reagent (CMAX00001; Thermo Fisher) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were labelled by conjugation with either Alexa Fluor 488 or 647 following maleimide chemistry (Invitrogen), as in (Picas et al. ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Recombinant PLD3 and PLD4 proteins were expressed in Expi293F cell line according to the manufacturer’s instructions (ThermoFisher). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/mL of recombinant protein was mixed with SYPRO Orange at a 5X final concentration (Invitrogen), and reactions were supplemented with 50 mM HEPES ...
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bioRxiv - Developmental Biology 2021Quote: Samples were analysed on GeneChip Zebrafish Gene 1.0 ST Array chips (902007, ThermoFisher) by the Genomics Lab of Dr ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from live embryos and adult zebrafish using Trizol (Invitrogen) and homogenised by pestle crushing and vortexing ...
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bioRxiv - Genetics 2019Quote: Total RNA from pools of 50 zebrafish embryos was extracted using Trizol (Ambion) and a RNeasy mini kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... The zebrafish samples were stored in TRIzol reagent (Invitrogen Corp., Carlsbad, CA, #15596018) and then purified with the Direct-zol RNA kit (Zymo Research ...
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bioRxiv - Neuroscience 2020Quote: Zebrafish larvae were immobilized with a 1mg/mL α-bungarotoxin (Thermo Fisher Scientific) solution for 3-5 minutes and rinsed off thoroughly with extracellular solution (134 mmol NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Larval zebrafish were added directly to 300 ml trizol solution (Invitrogen, Waltham, MA) and homogenized using a pellet pestle (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The zebrafish were then embedded in Shandon Cryomatrix embedding resin (Thermo Scientific, 6769006), and frozen sections were cut at 25 µm using a Leica CM3050S cryostat ...
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bioRxiv - Immunology 2022Quote: ... Both recombinant LPS (100ng/ml) and recombinant IFNy (10 ng/ml, Gibco) were provided to cells for differentiation to classical macrophages ...
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bioRxiv - Molecular Biology 2020Quote: ... Immulon 2 HB plates were coated with 2μg/ml Pierce recombinant protein A/G (ThermoFisher catalog no: 77677) or purified SARS-CoV-2 receptor binding domain (provided by Florian Krammer ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was collected 6 days later and recombinant mAb was purified using protein A agarose (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Recombinant STUB1 protein (aa25-aa153) was dialyzed overnight with Slide-A-Lyzer cassette (7K MWCO, Thermo Scientific, #66373) in 1 liter of dialysis buffer (PBS ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al., 2017) (Thermo Fisher Scientific). The cells were harvested 48–60 h after expression was induced in BMMY medium when 10 mM of all-trans-retinal (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was captured on a Ni2+-NTA HisPur® affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... Expression and purification of recombinant proteins were performed according to manufacturer’s instructions (Invitrogen Corporation, Catalog no. K1710-01): YPD medium supplemented with 100 μg ml−1 zeocin was used for initial growth of P ...
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bioRxiv - Biochemistry 2020Quote: The soluble recombinant protein was captured on a Ni2+-NTA affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with 25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... Dinutuximab and dinutuximab LALA-PG were then purified using recombinant protein A-Sepharose 4B (ThermoFisher Scientific, Waltham, MA), buffer exchanged into antibody buffer (20mM L-Histidine ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant RNase H (Invitrogen) was later added to the reverse transcribed samples and incubated for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Recombinant B18R (Life Technologies) was used at 1ug/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... zebrafish larvae at 3 dpf were incubated with LysoTracker Red DND-99 (Life Technologies) which contains fluorescent acidotropic probes for labeling and tracking of acidic organelles ...
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bioRxiv - Developmental Biology 2019Quote: ... 20 zebrafish larvae digestive tracts were dissected and pooled into 1mL TRIzol (ThermoFisher, 15596026). mRNA was then isolated with isopropanol precipitation and washed with 70% EtOH ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated in zebrafish medium containing 5μM DAF-FM DA (Life Technologies, D23842) for 30 minutes in the dark at 28ºC ...
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bioRxiv - Neuroscience 2021Quote: ... zebrafish larvae were immobilised in 2% low melting point agarose (Fisher Scientific, BP1360-100), placed on a glass coverslip and submerged in fish water ...
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bioRxiv - Neuroscience 2020Quote: ... zebrafish larvae were immobilised in 2% low melting point agarose (Fisher Scientific, BP1360-100), placed on a glass coverslip and submerged in fish water ...
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bioRxiv - Biophysics 2022Quote: Mfsd2a isoform B from zebrafish (Uniprot Q6DEJ6) was subcloned in the pFasbac1 vector (Invitrogen) with a N-terminal-10Xhis tag ...
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bioRxiv - Developmental Biology 2021Quote: ... xenopus (Sox18) or zebrafish (Nr2f2) genes were cloned into a pME Gateway vector (Invitrogen) and then recombined with the zebrafish ubi promoter68 ...
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bioRxiv - Neuroscience 2021Quote: ... zebrafish larvae were immobilised in 2% low melting point agarose (Fisher Scientific, BP1360-100), placed on a glass coverslip and submerged in fish water ...
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bioRxiv - Neuroscience 2019Quote: ... zebrafish larvae were immobilised in 2% low melting point agarose (Fisher Scientific, BP1360-100), placed on a glass coverslip and submerged in fish water ...
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bioRxiv - Molecular Biology 2020Quote: Adult zebrafish liver and heart tissue were homogenized in NP40 lysis buffer (ThermoFisher, FNN0021). Protein concentrations were estimated using BCA (Pierce 23225 ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from cells or zebrafish embryos using TRIzol™ (ThermoFisher, 15596081) reagent according to manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from zebrafish intestinal tissue using TRIzol™ Reagent (Invitrogen Cat# 15596026) as follows ...
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bioRxiv - Biochemistry 2024Quote: Whole-mount staining of live zebrafish larvae was performed using Yo-Pro-1 (Invitrogen) to observe neuromast hair cells ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco and used at the indicated concentrations ...
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bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...