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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Recombinant Sheep IL12B Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... A 1ug/mL mixture of 50μL per well containing recombinant protein in carbonate buffer (0.1M Na2CO3 0.1M NaHCO3 pH 9.3) was used to coat MaxiSorp (ThermoFisher) 96-well plates overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed and purified using a mammalian expression system via Expi293F cells (Life Technologies), as described in detail earlier (30 ...
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bioRxiv - Plant Biology 2022Quote: For recombinant protein expression in Pichia pastoris KM71H (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs encoding SCPSs ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant Streptococcus pyogenes Cas9 protein (SpCas9; Synthego) using Lipofectamine CRISPRMAX Cas9 Transfection reagent (CMAX00001; Thermo Fisher) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were labelled by conjugation with either Alexa Fluor 488 or 647 following maleimide chemistry (Invitrogen), as in (Picas et al. ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Recombinant PLD3 and PLD4 proteins were expressed in Expi293F cell line according to the manufacturer’s instructions (ThermoFisher). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/mL of recombinant protein was mixed with SYPRO Orange at a 5X final concentration (Invitrogen), and reactions were supplemented with 50 mM HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... donkey anti-sheep Alexa Fluor 647 (1:1000, Invitrogen, A32849) or donkey anti-rabbit Alexa Fluor 647 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor-488 donkey anti-sheep (1:500, Thermo Fisher Scientific Cat# A-11015 ...
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bioRxiv - Cell Biology 2019Quote: ... sheep anti-rat IgG antibodies (Invitrogen; 200 μg ml-1) were coated non-covalently with the antibodies of interest or covalently to Poly-D lysine (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... and Dynabeads bearing sheep anti-rabbit Ig (Thermo Fisher Scientific). H3K27ac ChIPs were prepared using the ChIP assay kit (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa 568 donkey anti-sheep IgG (Life Technologies No. A21099), and TRITC donkey anti-chicken IgY (Jackson No ...
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bioRxiv - Neuroscience 2019Quote: ... with Dynabeads Sheep Anti-Rat IgG (Invitrogen, cat. no. 11035) for 35 min ...
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bioRxiv - Cell Biology 2021Quote: ... 300 ul of M280 sheep anti-rabbit IgG beads (ThermoFisher) was washed with cold BSA/PBS (0.5 mg / mL bovine serum albumin in 1x phosphate buffered saline ...
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bioRxiv - Neuroscience 2020Quote: ... HRP-conjugated rabbit of sheep secondary antibodies (1:3000 ThermoFisher) and HRP-conjugated goat of rabbit secondary antibodies (1 ...
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bioRxiv - Neuroscience 2020Quote: ... coupled with Dynabeads Sheep anti-Rat IgG (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... 50 μl sheep anti-mouse magnetic beads (Thermo Fisher Scientific) that had previously been linked with 4 μg each of mouse monoclonal 6E10 and 4G8 (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 568-conjugated anti-sheep IgG (1:1000, Invitrogen). DAPI (1:2000 ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-sheep IgG (H+L) were purchased from Thermo Fisher Scientific Inc ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated anti-sheep (1:5000, Invitrogen, cat. no. A16041).
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bioRxiv - Cancer Biology 2021Quote: ... Dynabeads M-280 Sheep Anti-Rabbit IgG (Thermo Fisher Scientific) coupled to either a rabbit IgG control or an anti-Nucleolin antibody (Cell Signaling Technology ...
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bioRxiv - Genomics 2020Quote: ... 300 ul of M280 sheep anti-rabbit IgG beads (ThermoFisher) was washed with cold BSA/PBS (0.5 mg / mL bovine serum albumin in 1x phosphate buffered saline ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor 488 Donkey anti-Sheep IgG (Thermo Fisher Scientific) was used as secondary antibody at 1:500 in blocking solution and incubated for 1 h ...
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bioRxiv - Immunology 2022Quote: ... with addition of 5% defibrinated sheep blood (Thermo Scientific®). When required ...
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bioRxiv - Neuroscience 2023Quote: ... We used sheep anti-TENM2 (1:100, Invitrogen, #PA5-47638) and sheep anti-SORCS2 (1:500 ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... anti-rabbit and anti-sheep secondary antibodies (Invitrogen - 1:400). 0.5 µg/mL 4’ ...
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bioRxiv - Cell Biology 2023Quote: ... donkey anti-sheep AlexaFluor 647 (Thermo Fisher, A21448, 1:1000). Images were acquired on a spinning disc Eclipse Ti2 inverted microscope (Nikon Instruments Inc) ...
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bioRxiv - Neuroscience 2023Quote: ▪ Alexa568-donkey-anti-sheep (A-21099, Thermo Fisher 1:500)
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-sheep Alexa 647 (1:1000, A-21448, Thermofisher) was used ...
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bioRxiv - Neuroscience 2023Quote: ▪ Alexa488-donkey-anti-sheep (A-11015, Thermo Fisher, 1:500)
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bioRxiv - Genomics 2023Quote: ... and lineage depleted using sheep anti-rat Dynabeads (Invitrogen, 1135) on a magnet ...
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bioRxiv - Systems Biology 2024Quote: ... conjugated magnetic beads with polyclonal sheep anti-rabbit IgG (Invitrogen) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... conjugated to Dynabeads M-280 sheep anti-mouse IgG (ThermoFisher) for 1 hour at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... or donkey anti-sheep-AlexaFluor568 ABs (Thermo Scientific, cat.# A21099) were used except for the V5 condition ...
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bioRxiv - Immunology 2022Quote: ... Both recombinant LPS (100ng/ml) and recombinant IFNy (10 ng/ml, Gibco) were provided to cells for differentiation to classical macrophages ...
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bioRxiv - Molecular Biology 2020Quote: ... Immulon 2 HB plates were coated with 2μg/ml Pierce recombinant protein A/G (ThermoFisher catalog no: 77677) or purified SARS-CoV-2 receptor binding domain (provided by Florian Krammer ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was collected 6 days later and recombinant mAb was purified using protein A agarose (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Recombinant STUB1 protein (aa25-aa153) was dialyzed overnight with Slide-A-Lyzer cassette (7K MWCO, Thermo Scientific, #66373) in 1 liter of dialysis buffer (PBS ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al., 2017) (Thermo Fisher Scientific). The cells were harvested 48–60 h after expression was induced in BMMY medium when 10 mM of all-trans-retinal (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was captured on a Ni2+-NTA HisPur® affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... Expression and purification of recombinant proteins were performed according to manufacturer’s instructions (Invitrogen Corporation, Catalog no. K1710-01): YPD medium supplemented with 100 μg ml−1 zeocin was used for initial growth of P ...
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bioRxiv - Biochemistry 2020Quote: The soluble recombinant protein was captured on a Ni2+-NTA affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with 25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... Dinutuximab and dinutuximab LALA-PG were then purified using recombinant protein A-Sepharose 4B (ThermoFisher Scientific, Waltham, MA), buffer exchanged into antibody buffer (20mM L-Histidine ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant RNase H (Invitrogen) was later added to the reverse transcribed samples and incubated for 20 min at 37°C ...