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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and then Fc receptors were blocked with CD16/CD32 antibody (Invitrogen). Cells were then surface stained with fluorochrome-labeled mAbs specific for mouse CD4 ...
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bioRxiv - Cell Biology 2019Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked by incubating cells with 1:50 anti- mouse CD16/CD32 (Fc block, Invitrogen) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Immunology 2022Quote: ... followed by Fc-receptor blockade with anti-CD16/CD32 (Thermo Fisher #14-0161-85), and then stained for 30 min on ice with the following antibodies in flow cytometry staining buffer (FACS) ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with anti-CD16/CD32 (diluted 1:50, Thermo Fisher Scientific) and cells were stained with cell surface antigens ...
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bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked with anti-mouse CD16/CD32 (20 µg/ml, clone 93; Invitrogen) for 20min ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Immunology 2023Quote: ... cells were first incubated with an anti-CD16/CD32 Monoclonal Antibody to block FC receptors (Invitrogen). Cells were washed using PBS and incubated with LIVE/DEAD™ dye for 30 minutes at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant 6x-His tagged PTB was purified using Ni-NTA agarose (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... pH 7.2) resuspended and incubated for 30 min in FACS buffer supplemented with Fc gamma receptor CD16/CD32 antibodies (ThermoFisher). Cells were then incubated with Alexa fluor 488-conjugated antibodies against F4/80 or rat IgG2a kappa isotype control ...
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bioRxiv - Immunology 2024Quote: ... Fcγ receptors were blocked using murine αCD16/CD32 (ThermoFisher), human FcR Blocking (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... Fc block (anti-CD16/CD32, 101302 Affymetrix) was included to minimize nonspecific antibody binding ...
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bioRxiv - Immunology 2022Quote: ... Fc blocking (Anti CD16/CD32, Invitrogen, US) was performed on all samples prior to extracellular antibody staining ...
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bioRxiv - Bioengineering 2023Quote: ... Fc blocking (Anti CD16/CD32, Invitrogen, US) was performed on all samples prior to extracellular antibody staining ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were excluded using ThermoFisher LIVE/DEAD Fixable Aqua Dead Cell Stain and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Absolute cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Immunology 2020Quote: Up to 5×106 isolated cells were incubated for 30 min at 4 °C with anti-CD16/CD32 (Fc receptor) clone 93 (Invitrogen) to block non-specific binding and with fixable viability dye eFluor455UV (eBioscience ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
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bioRxiv - Molecular Biology 2020Quote: ... and goat anti-rat lgG Alexa Fluor 633 (each 1:1000, Invitrogen). Samples were incubated in secondary antibodies for 1 day at 4°C with gentle rotation ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 100 µL FACS buffer supplemented with Fc-γ receptor anti-CD16/CD32 antibodies (1:100, Thermo Fisher Scientific) and incubated on ice at 4°C for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... and goat anti-rat lgG conjugated to Alexa 647 (1:500, Invitrogen, USA) were used as secondary antibodies ...
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bioRxiv - Immunology 2022Quote: ... and FcR (Fc receptor) true block (Invitrogen) for 12 minutes at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged protein was purified from the cleared cell lysate by nickel affinity chromatography (Ni-NTA agarose beads, Invitrogen). After washing with increasing concentrations of imidazole ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Biochemistry 2022Quote: ... His6- tagged SPOC domains were purified by affinity chromatography using HisTrap HP column (Cytiva) or His-Pur Ni-NTA resin (Thermo Scientific) equilibrated in 25 mM Tris-Cl pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... p38β (MAPK11, His-tagged, Thermo Fisher Scientific, #PV3679), p38γ (MAPK12 ...
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bioRxiv - Cell Biology 2019Quote: ... p38γ (MAPK12, His-tagged Thermo Fisher Scientific, #PV3654), p38d (MAPK13 ...
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bioRxiv - Cell Biology 2019Quote: ... p38d (MAPK13, His-tagged, Thermo Fisher Scientific, #PV3656) and hPI31 (UBPBio ...
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bioRxiv - Immunology 2023Quote: ... 6x His Tagged monoclonal antibody (Thermo Fisher Scientific) was also plated as an experimental control ...
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bioRxiv - Microbiology 2021Quote: ELISA assays were performed in 96-microwell plates coated with 150 ng/well of SARS-CoV-2 Spike (aa 16-1213) His-tagged recombinant protein (ThermoFisher) and 1010 phage or AAVP particles/50 μL of PBS O.N ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then purified by immobilized metal affinity chromatography by using HisPur Ni-NTA (Purify His-tagged recombinant fusion proteins using high-capacity nickel-nitrolotriacetic acid methal-chelate beads) spin columns (ThermoFisher). Recombinant proteins were refolded on the column by gradually removing urea until reaching to a concentration 0 M and then dialyzed against PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... with 10 % V/V Hi FCS (Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... with Fc Receptor Binding Inhibitor (Thermo Fisher Scientific, MA) for 20 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
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bioRxiv - Immunology 2020Quote: ... Cells were Fc blocked with a CD16/CD32 antibody (ThermoFisher, 14-0161-82) for 10 minutes at 4°C and then incubated for 30 min at room temperature with primary antibodies including CD45-BV785 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Cells were Fc blocked with a CD16/CD32 antibody (ThermoFisher, 14-0161-82) for 10 minutes at 4°C and then incubated for 30 min on ice with primary antibodies including CD45-PE (30-F11 ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Cells were Fc blocked with a CD16/CD32 antibody (ThermoFisher, 14-0161-82) for 10 minutes at 4°C and then incubated for 30 min on ice with primary antibodies including CD45-PE ...
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bioRxiv - Cell Biology 2021Quote: Purified recombinant GST-tagged CDK1-cyclin A2 and His6-tagged CDK1-cyclin B1 (Invitrogen) were stored in 150 mM NaCl ...
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bioRxiv - Systems Biology 2020Quote: ... the GST-Tagged proteins were eluted with 15 mM GSH (Bio-basic) and cleaved in solution using purified recombinant His-Tagged TEV protease (Thermo Fisher Scientific). Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare ...