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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Recombinant Pig SERPINA6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Immulon 2 HB plates were coated with 2μg/ml Pierce recombinant protein A/G (ThermoFisher catalog no: 77677) or purified SARS-CoV-2 receptor binding domain (provided by Florian Krammer ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was collected 6 days later and recombinant mAb was purified using protein A agarose (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Recombinant STUB1 protein (aa25-aa153) was dialyzed overnight with Slide-A-Lyzer cassette (7K MWCO, Thermo Scientific, #66373) in 1 liter of dialysis buffer (PBS ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al., 2017) (Thermo Fisher Scientific). The cells were harvested 48–60 h after expression was induced in BMMY medium when 10 mM of all-trans-retinal (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was captured on a Ni2+-NTA HisPur® affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... Expression and purification of recombinant proteins were performed according to manufacturer’s instructions (Invitrogen Corporation, Catalog no. K1710-01): YPD medium supplemented with 100 μg ml−1 zeocin was used for initial growth of P ...
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bioRxiv - Biochemistry 2020Quote: The soluble recombinant protein was captured on a Ni2+-NTA affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with 25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... Dinutuximab and dinutuximab LALA-PG were then purified using recombinant protein A-Sepharose 4B (ThermoFisher Scientific, Waltham, MA), buffer exchanged into antibody buffer (20mM L-Histidine ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant RNase H (Invitrogen) was later added to the reverse transcribed samples and incubated for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Recombinant B18R (Life Technologies) was used at 1ug/ml ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco and used at the indicated concentrations ...
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bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...
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bioRxiv - Microbiology 2022Quote: Recombinant proteins were produced in transiently transfected suspension culture of FreeStyle 293 cells (Thermo Fisher Scientific, Waltham, MA, USA), as previously described 66 ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Immunology 2021Quote: The recombinant chimera CD14-Fc protein was biotinylated using the EZ-Link Sulfo NHS-LC-LC-Biotin kit (Thermofisher) and a 5-fold molar excess of biotin ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Immunology 2020Quote: ... The vector was linearized with BglII and transformation of yeast (Pichia pastoris) and induction of recombinant protein expression was carried out according to the manufacturer’s instructions (Invitrogen). Recombinant HELMCC protein was purified from yeast culture supernatants by ionexchange chromatography using Ni2+ affinity chromatography (Amersham Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... Purified soluble recombinant proteins were tested for bacterial endotoxins using the Limulus Amebocyte Lysate (LAL) assay kit (Invitrogen, USA).
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bioRxiv - Biochemistry 2022Quote: ... Pierce™ recombinant protein A/G and clear round-bottom immuno nonsterile 96-well plates were obtained from ThermoFisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Cells were lysed by sonication and recombinant proteins purified by Ni2+ affinity chromatography in Ni-NTA agarose (Thermo Fisher) using 50 mM TrisHCl/500 mM NaCl/10 mM imidazole [ph 8.0] as binding buffer ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Biochemistry 2023Quote: ... The coding sequences of the recombinant proteins were then inserted into the BamHI and EcoRI sites of the pcDNA3.1(+) vector (Invitrogen), and all clones were verified through DNA sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... Purity of recombinant proteins was verified by SDS-PAGE with staining by GelCode Blue stain reagent (Thermo Fisher scientific).
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bioRxiv - Neuroscience 2024Quote: ... A polyclonal antibody to Drosophila tau was prepared in rabbits immunized with full length recombinant tau protein (Thermo Fisher) and was used at 1:5,000,000 for western blotting ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) as previously described30 using HisPur cobalt resin (Thermo Scientific). Before elution ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2020Quote: Guinea pig anti-Cad [63] (1:400) and Alexa Fluor conjugated 647 donkey anti-guinea pig (1:500) (Molecular Probes Cat# A-21447, RRID:AB_141844) were used to examine Cad protein expression ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-guinea pig 647 (1:500, ThermoFisher A-21450), goat anti-rabbit 647 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 647 goat anti-guinea pig (#A21450, ThermoFisher Scientific) and DAPI (#62248 ...
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bioRxiv - Immunology 2021Quote: ... and goat anti-guinea pig AlexaFluor® 647 (#A21450, ThermoFisher). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa-488 anti-guinea pig (Molecular Probes, Eugene, OR, USA) or Alexa-488 anti-rabbit (Molecular Probes)] ...
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bioRxiv - Neuroscience 2021Quote: ... anti-guinea pig conjugated to Alexa 568 (A11075, Thermo Fisher); anti-mouse conjugated to either Alexa 488 (A11001 ...
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bioRxiv - Physiology 2021Quote: ... anti- guinea pig Alexa-594 (1:200, Invitrogen #A-11076). Samples were mounted with either VECTASHIELD Hard Set Mounting Medium (Vector labs ...
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bioRxiv - Physiology 2021Quote: ... anti-guinea pig Alexa-594 (1:200, Invitrogen #A-11076). Samples were mounted with either VECTASHIELD Hard Set Mounting Medium (Vector labs ...
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bioRxiv - Cell Biology 2021Quote: ... and goat-anti guinea pig (ThermoFisher A-21450, 1:1000).
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bioRxiv - Microbiology 2021Quote: ... and anti-guinea pig Alexa 488 (1:400; Invitrogen, A11073). Coverslips were then washed three times with PBS.
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bioRxiv - Biochemistry 2022Quote: ... goat anti-guinea pig Alexa Fluor 647 (Invitrogen, A-21450). All washing steps were performed with PHEM-T buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and HRP conjugated goat anti-guinea pig IgG (Thermo Fisher) 1:20000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor 594 goat anti-guinea pig (Invitrogen, Waltham, MA), Alexa Fluor 594 donkey anti-rat (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... goat anti-guinea pig alexa 555 (1:200) (Invitrogen, A21435). Proper fluorophore-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Goat anti Guinea pig IGG-488 (Invitrogen Catalog number: A11073) was used at a dilution of 1:1000 ...
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bioRxiv - Physiology 2020Quote: WT pig fetal fibroblasts (PFF) were transfected (Lipofectamine LTX (Invitrogen)) with a plasmid containing the hα-Blast sequence (ensemble reference ENSG00000206172.8 ...
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bioRxiv - Cell Biology 2022Quote: ... and goat anti-guinea pig HRP (1:5000, ThermoFisher #A18769). Densitometry was measured in Adobe Photoshop and protein levels are normalized to the Asl loading control ...
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bioRxiv - Cell Biology 2022Quote: ... goat anti-guinea pig IgG antibody AlexaFluor488 (A-11073, Invitrogen), goat anti-mouse IgG antibody AlexaFluor555 (A-21422 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were guinea pig anti-TRPV1 (ThermoFisher, 1:200), rabbit anti-CGRP (Neuromics ...