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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Recombinant Mouse Vsig4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Recombinant His-HMGB1 was produced in 293-freestyle cells (ThermoFisher Scientific). Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant CEACAM-His proteins were expressed in Expi293F cells (Life Technologies) and purified by affinity chromatography (ÄKTA Pure ...
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bioRxiv - Immunology 2020Quote: ... and 10 % V/V heat-inactivated foetal calf serum (Hi FCS, Gibco). On day 1 ...
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bioRxiv - Immunology 2022Quote: ... with 10% v/v heat inactivated foetal calf serum (HI-FCS, Gibco), 1 mM sodium pyruvate and 1X non-essential amino acids (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Mouse-anti-His (HIS.H8) antibody (Invitrogen) (for eGFP/His-tagged NP protein co-immunoprecipitation ...
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bioRxiv - Biochemistry 2021Quote: ... His (1:1000, Invitrogen, mouse monoclonal), Flag M2(1:1000 ...
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bioRxiv - Synthetic Biology 2022Quote: All antigens were His-tagged and purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer [20 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... HA-tagged RON was cloned into the expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) by fusion PCR ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher). To eliminate nonspecific binding proteins ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged hAgo2 was purified from Sf9 cells using a baculovirus system (Thermo Fisher). Cells were lysed in Lysis Buffer (300mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged protein was labeled with Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc (18-615) protein expressed in Expi293F (Life Technologies) cells was chemically biotinylated using EZ-link Sulfo-NHS-Biotin (A39256 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% mouse recombinant EGF (Invitrogen, 5% Recombinant human R-spondin (Peprotech).
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bioRxiv - Cell Biology 2020Quote: ... His6-tagged recombinant proteins were produced in BL21 (DE3) grown in MagicMedia (Invitrogen) 24h ...
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bioRxiv - Molecular Biology 2021Quote: ... and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific). On-bead recombinant proteins were washed with the respective lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: Recombinant GST and GST-tagged EB1 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.7 and induced with 1 mM IPTG for 5.5 h at 25°C ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10 % V/V heat inactivated Fetal Calf Serum (Hi FCS, Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (hi-FCS) (Gibco) and 1% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... medium containing 10% (v/v) heat inactivated fetal calf serum (HI-FCS; Gibco), 100 U/mL Penicillin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10 % v/v heat-inactivated fetal calf serum (HI-FCS, Gibco), 1 mM non-essential amino acids (NEA ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged RRs were purified from clarified lysate using HisPur Ni-NTA resin (Thermo Scientific) or HisPur Cobalt resin (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr: 11533923, Fisher Scientific), a HRP-coupled secondary antibody (ArtNr ...
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bioRxiv - Neuroscience 2023Quote: ... The His-tagged dAsapPZA protein was expressed and purified following the manufacturer’s guidelines (Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... His-tagged LCRIS_00661 and LCRIS_00558 genes with the appropriate restriction sites were ordered from Invitrogen and subcloned into a previously constructed S ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-His (MA1-21315, Thermo Scientific), mouse anti-β-actin HRP (Santa Cruz Biotechnologies ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-His Tag (Invitrogen MA1-21315). Secondary antibody used are ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal anti-6x-His (Thermo Fisher) diluted 1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Immunology 2019Quote: ... we added recombinant mouse IFNγ (ThermoFisher), 2-deoxyglucose (Abcam) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse recombinant Egf (Life Technologies #PMG8043), 100 ng/mL mouse recombinant Noggin (Peprotech #250-38 ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... Sybr Safe DNA gel stain and BenchMark™ His-tagged Protein Standard were purchased from Invitrogen. HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech ...
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bioRxiv - Evolutionary Biology 2020Quote: ... His-tagged proteins were eluted from the beads in a disposable polypropylene column (29924, ThermoFisher Scientific) using elution buffer (4 ml wash buffer containing 400 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... media was collected and His tagged proteins were bound using HisPur Ni-NTA Resin (ThermoFisher; 88221) in the presence of Calbiochem’s EDTA-free protease inhibitor cocktail 1:1000 (MilliporeSigma ...
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bioRxiv - Biochemistry 2021Quote: 1-2 μg His-tagged calcineurin was first bound to magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... His-tagged AtKu70/80 was isolated from the supernatant using HisPur Ni-NTA resins (Thermo Fisher) and dialysed in 20 mM HEPES pH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The His tagged RBD construct was purified from the culture supernatant of Expi293 cells (Thermo Fisher) that were transfected with the plasmid DNA using FectoPRO (Polyplus Inc.) ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-His antibody (Thermo Fisher, Waltham, MA) was diluted 1:2000 in 1x iBind solution ...
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bioRxiv - Microbiology 2022Quote: ... and mouse anti His Alexa Fluor 555 (Invitrogen catalogue number MA1-135-A555 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-His (Invitrogen, #MA1-21315, 1:1000), rabbit anti-GST (Merck,#ZRB1223,1:1000) ...
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bioRxiv - Microbiology 2023Quote: ... anti-6x-His mouse monoclonal (Invitrogen 37-2900), anti-CD99 mouse monoclonal (Invitrogen MA5-12954) ...
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bioRxiv - Biochemistry 2021Quote: GST-tagged p107 constructs loaded on beads were phosphorylated with recombinant Cyclin A/CDK2 (Invitrogen) in 200 nM ATP KAS buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Alexafluor-594 tagged recombinant cholera toxin subunit B (CTB-red) was also purchased from Invitrogen Molecular Probes and used as per manufacturer’s recommendations (10 µM final concentration).
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bioRxiv - Molecular Biology 2023Quote: GST-tagged p107 constructs loaded on beads were phosphorylated with recombinant cyclin B/CDK1 (Invitrogen) in 200 nM ATP KAS buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme and Fc-portion was removed on HIS-Pur Ni-NTA resin (Thermo Fisher Scientific) and Protein G sepharose (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: Mono-Fc tagged PvDBP-RII used for SAXS experiments was expressed in Expi293 cells (Thermo Scientific) as described above and purified using the CaptureSelect C-tagXL Affinity Matrix (Thermo Scientific) ...