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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Recombinant Mouse SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
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bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...
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bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Biochemistry 2022Quote: Recombinant hItln1 and MBL were characterized by western blotting using monoclonal mouse anti-MBL2 antibody (3E7; ThermoFisher Scientific MA1-40145; 1:50) and polyclonal rabbit anti-hItln1 antibody (Proteintech 11770-1-AP ...
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bioRxiv - Microbiology 2021Quote: ... A mouse monoclonal anti-HSV 45-kDa protein (scaffolding protein) antibody (Serotec) at 1:2000 dilution and Alexa Fluor 568–goat anti-mouse IgG (Invitrogen) at 1:1000 dilution were used as primary and secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... were then subjected to preclearing with 20 μL of either magnetic protein G (for Rabbit antibodies) or Protein A (for Mouse antibodies) Dynabeads (Invitrogen) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... INS1 cells labeled with Bodipy C12 were also immunostained with mouse anti-HSP60 antibody (66-41-1-lg, Protein Tech) and Alexa Fluor 488 goat anti-Mouse IgG (A11017, Invitrogen) both at 1:300 ...
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bioRxiv - Immunology 2022Quote: ... the wells were incubated with PBS (control) or serially-diluted irrelevant mouse IgG (anti-green fluorescence protein of mouse IgG, ThermoFisher) or irrelevant rabbit IgG (anti-green fluorescence protein of rabbit IgG ...
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bioRxiv - Microbiology 2021Quote: ... For immunofluorescence analysis the cells were fixed as described above and stained with a monoclonal mouse antibody against the viral capsid proteins followed by staining with a goat anti-mouse IgG Alexa Fluor 488 (Invitrogen).
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bioRxiv - Plant Biology 2020Quote: ... Dynabeads M280 Sheep anti-mouse IgG or Dynabeads Protein G (Thermo Fisher Scientific) were used for immunoprecipitation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... pre-coated with laminin mouse protein at 2μg/cm2 (Thermo Fisher Scientific, 23017015) and maintained in the culture medium with 2% CO2 (24) ...
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bioRxiv - Microbiology 2020Quote: ... Polyclonal antibodies were purified from mouse sera using Protein A Sepharose-4B (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... the culture dish is coated with laminin mouse protein (20μg/mL, Gibco, 23017015) for another 24 hours and wash three times with water to remove redundant laminin mouse protein ...
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bioRxiv - Molecular Biology 2019Quote: ... or 15 μl protein goat-anti mouse Ig magnetic (112.01D,Thermo Fisher Scientific) for Tau (both types of beads were pre-blocked in 5% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... or normal mouse IgG (santa cruz) coupled to magnetic protein G beads (Invitrogen) for 2 h at 4 °C under constant agitation ...
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bioRxiv - Cell Biology 2020Quote: ... or normal mouse IgG (santa cruz) coupled to magnetic protein G beads (Invitrogen) for 3 - 4 h at 4 °C under constant agitation ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... Polyclonal antibodies were purified from mouse sera using Protein A Sepharose-4B (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized by western blotting with mouse anti-Strep (Invitrogen/Thermo Fisher) and IRDye-800CW anti-mouse IgG (Licor) ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized by western blotting with mouse anti-Strep (Invitrogen/Thermo Fisher) and IRDye-800CW anti-mouse IgG (Licor) ...
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bioRxiv - Plant Biology 2022Quote: ... protein was detected using monoclonal mouse Anti-myc (1:7,500; R950-25, Thermofisher), polyclonal rabbit anti-GFP (1:7,500 ...
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Immunology 2023Quote: ... Resulting proteins were purified via CaptureSelect LC-lambda (mouse) affinity matrix (ThermoFisher, 194323010) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... the wild-type and mutant (C302/305R) recombinant Mul1 proteins were produced in Sf9 cells using the Bac-to-Bac Baculovirus expression system (Invitrogen, Carlsbad, CA, USA) and purified by a nickel-chelating resin (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: In vitro binding experiments were performed with recombinant GST fusion proteins purified from E.coli and immobilized on glutathione-Sepharose 4B beads (Life Technologies #17-0756-01). Purification of GST fusion proteins was performed as described (Ebnet et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The same recombinant Cdk5/p25 protein used for the MST assays was labeled with biotin using EZ-LinkNHS-PEG4-Biotinylation Kit (Thermo Scientific, Waltham, MA) and excess reagent was removed with a spin desalting column as per the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... A concentration range of indicated recombinant proteins was analyzed by thermal melt assays in SEC buffer using SYPRO Orange (Molecular Probes, cat.# S6651) using a Biorad CFX96 Touch Real-Time PCR C1000 System (ramping from 4 to 100°C at 1°C per minute).
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bioRxiv - Microbiology 2020Quote: ... recombinant proteins were purified by affinity chromatography as described previously (27) and loaded on NuPAGE 4-12% BisTris gels (Invitrogen, Carlsbad, CA, USA) to confirm the correct molecular weights and assess their purity.
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876-79). The recombinant neuraminidase (NA ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/mL recombinant murine EGF (Gibco, PMG8043), 100ng/mL recombinant human Noggin (Peprotech ...
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bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human GDNF (Thermo Fisher), 10ng/mL recombinant human CNTF (Thermo Fisher)).
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bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human BDNF (Thermo Fisher), 10ng/mL recombinant human GDNF (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human CNTF (Thermo Fisher)).
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bioRxiv - Cell Biology 2019Quote: ... coli-derived recombinant human insulin (Gibco, A11382IJ), 64 μg ml−1 L -ascorbic acid 2-phosphate trisodium salt (Wako ...
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bioRxiv - Bioengineering 2021Quote: ... on recombinant human vitronectin (50μg/ml; Gibco) coated plates ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/ml recombinant EGF (Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
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bioRxiv - Immunology 2022Quote: ... The recombinant ORF8 (ThermoFisher, Cat. RP-87666) was conjugated with FITC using the FITC conjugation kit (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) was added to lysate (1ul per 100ul lysate) ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 ng/ml human recombinant EGF (Invitrogen), 10 ng/ml bFGF (R&D Systems ...
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bioRxiv - Genomics 2019Quote: ... 0.25 μL RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen), was then added to each well ...
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bioRxiv - Cancer Biology 2020Quote: ... purified recombinant human IL-8 (ThermoFisher Scientific) and CXCL1 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant Cas9 2.0 (Thermo Fisher Scientific, A36498) was mixed with gRNA and incubated for 15 min at room temperature ...
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Differential expression of transposable elements in stem cell lineages of the preimplantation embryobioRxiv - Developmental Biology 2020Quote: ... 25 ng/mL human recombinant FGF4 (Gibco), and 1 μg/mL heparin (Sigma-Aldrich)] as previously described (Tanaka et al. ...
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bioRxiv - Cell Biology 2021Quote: ... 0.15 ng of human recombinant VEGF (Gibco) were co-injected with 1.0×106 cells of WS-iMSC.
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bioRxiv - Neuroscience 2021Quote: ... Recombinant pSinRep5 and helper plasmid pDH26S (Invitrogen) were transcribed in vitro into capped RNA using the Megascript SP6 kit (Ambion) ...
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bioRxiv - Genetics 2019Quote: ... 10 ng/ml recombinant human FGF (Gibco), 10 ng/ml recombinant human EGF (Peprotech ...