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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Recombinant Mouse Regenerating Islet derived 3 Alpha His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 50 ng/ml recombinant mouse epidermal growth factor (EGF; Gibco, PMG8041), 100 ng/ml recombinant murine Noggin (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% penicillin/streptomycin and 50ng/ml recombinant mouse EGF (Life Technologies) was used for culturing ApcKO colon organoids ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin/streptomycin and 50ng/ml recombinant mouse EGF (Life Technologies) was used for culturing colon organoids ...
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: Recombinant 6XHis-tagged TbVps24 (N-terminus tag) was cloned using the pTrcHis TOPO TA expression system (Thermo Fisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Insect cell derived recombinant tag-less eIF2A was expressed in ExpiSf9 cells using the ExpiSf Expression System Starter Kit (Thermo Fisher # A38841) following the manufacturer’s protocol and adapting the IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag ...
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-mouse IgG (H+L) antibody (Invitrogen), before being acquired with BD FACSCanto II (BD Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... SV5-tagged proteins were detected using mouse anti-SV5 mAb (1:5000, Life Technologies); HA-tagged proteins were detected by anti-HA mAb (clone HA-7 ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged scFvs were detected using mouse mAb 2-2.2.14 (ThermoFisher Catalog# P126183, RRID:AB_10978021). The following day sections were washed 4 x 5 min with vehicle ...
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bioRxiv - Cell Biology 2021Quote: ... The isolated mouse islets were cultured in Connaught Medical Research Laboratories (CMRL) 1066 medium (Invitrogen, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fatal calf serum ...
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bioRxiv - Cell Biology 2021Quote: ... The isolated mouse islets were cultured in Connaught Medical Research Laboratories (CMRL) 1066 medium (Invitrogen, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fatal calf serum ...
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bioRxiv - Developmental Biology 2019Quote: ... Alexa Flour 647-conjugated anti-mouse antibody (A31571) (all donkey-derived, 1:500, Molecular Probes). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse-derived osteoblast precursor cell line MC3T3 E1 was cultured in MEMα medium (Gibco™) containing 10% fetal bovine serum and 1% Penicillin-Streptomycin (Gibco™).
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... mouse anti-Galectin 3 (Thermo Fisher Scientific Cat# MA1-940 ...
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bioRxiv - Microbiology 2020Quote: ... in MEM-alpha (Gibco) with 20% FBS (Hyclone) ...
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bioRxiv - Bioengineering 2023Quote: ... in alpha-MEM (Gibco). MSCs were cultured to 80% confluence following standard protocols (65) ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1.5ml of SD/-Leu/-His liquid medium (plus 3 mM 3-AT) were incubated in a 12-well microplate (Thermo Scientific) at 30°C for 96 hours (h) ...
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bioRxiv - Immunology 2022Quote: ... The plate was coated with 2 µg/mL mouse anti-His antibody (Invitrogen cat ...
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bioRxiv - Bioengineering 2021Quote: ... Blocking buffer was refreshed and mouse anti-His antibody (Thermofisher, Cat#MA1-21315) was added at a dilution factor of 1:2000 in blocking buffer ...
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bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged goat anti-mouse IgG or Alexa Fluor 750-tagged goat anti-rabbit IgG (1:100, Thermo Fisher, cat# A21235 and A21039), or Alexa Fluor 647-tagged donkey anti-mouse IgG ...
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bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
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bioRxiv - Synthetic Biology 2020Quote: ... and the His-tagged proteins on the cell surface were then visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA) using FUSION Solo S documentation system (Vilber ...
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y cells were transfected with either an empty vector or with a vector expressing His-tagged human p35 (gift from Dr. Harish Pant) using Lipofectamine 3000 (Life Technologies, Carlsbad, CA). Two days after the transfection ...
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bioRxiv - Cell Biology 2022Quote: ... The homogenate was washed HBSS four times and islets were handpicked into islet media-G11 {RPMI-1640 media with 11 mM glucose (Gibco, Dublin, Ireland) plus 10% heat-inactivated Fetal Bovine serum [HI-FBS] (Atlanta Biologicals ...
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bioRxiv - Cancer Biology 2022Quote: ... TNF alpha mouse high sensitivity ELISA kit and IL-6 mouse high sensitivity ELISA kit were from Invitrogen (Thermo Fisher Scientific), mouse CCL2/JE/MCP-1 quantikine ELISA kit ...
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bioRxiv - Cell Biology 2022Quote: Human-derived derived near-haploid HAP1 cells were cultured in IMDM (GIBCO) supplemented with 12% FCS ...
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bioRxiv - Developmental Biology 2021Quote: ... kcnk5b cDNA was isolated by RT-PCR from regenerating adult fin cDNA library and cloned into MCS region of pcDNA6-myc-6xHIS-tag plasmid (Invitrogen) or pBluescript harboring the hsp70 zebrafish promoter and GFP coding sequence surrounded by 2 miniTol2 sites ...
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bioRxiv - Cell Biology 2020Quote: ... Islets were shipped in CMRL media (Thermo Fisher Scientific) overnight from Edmonton to the University of British Columbia ...
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bioRxiv - Molecular Biology 2020Quote: ... Islet were cultured in CMRL1066 (Thermo Fisher Scientific, 11530037) medium with 1% human serum albumin ...
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Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetesbioRxiv - Cell Biology 2019Quote: ... Pooled islets were washed with sterile PBS (Fisher Scientific) and incubated in Accutase cell detachment solution (Sigma ...
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bioRxiv - Physiology 2021Quote: ... dispersed islets were seeded in chamber slides (ThermoFisher Scientific) coated with poly-D-lysine hydrobromide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Islets were then dissociated using TrypLE Express (Life Technologies) and cell counts were obtained using an automated cell counter (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... derived from pcDNA5 (Invitrogen) and containing the wild type (wt ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... fixed oocytes were first incubated with a mouse monoclonal against alpha-tubulin (LifeTechnologies-Invitrogen, 13-800), followed by an incubation with chicken anti-mouse IgG conjugated to Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The MUTZ-3 cell line (DSMZ, no ACC 295) was cultured in Minimum Essential Medium Alpha (MEMα) (Gibco, Thermo Fischer Scientific Cat# 22571020 ...
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bioRxiv - Cell Biology 2019Quote: ... 20 ng/ml recombinant mouse macrophage colony-stimulating factor (Gibco, Burlington, ON), and penicillin/streptomycin antibiotics ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 µg/ml mouse recombinant epidermal growth factor (EGF; Thermo Fisher Scientific), 10nM [Leu15]-gastrin I (Merck) ...
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bioRxiv - Physiology 2021Quote: ... 100-300 islets from each mouse shipped in a blinded manner overnight to the University of Alberta in RPMI (Invitrogen, 11875) with 10% FBS (Invitrogen #12483020) ...
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bioRxiv - Immunology 2019Quote: ... Islet isolation was done courtesy of the UCSF Mouse Islet Isolation Facility [62] and then dissociated in Cell Dissociation Buffer Enzyme-Free PBS-based (ThermoFisher Scientific) at 37°C in a tissue culture incubator ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...