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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Recombinant Mouse PVR Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... The samples were mixed with recombinant protein G-Sepharose 4B beads (Thermo Fisher) and 1 µl anti-Flag antibodies (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: This recombinant S protein was overexpressed using the HEK 293F mammalian cells (Invitrogen) at 37°C under 5% CO2 in a Multitron-Pro shaker (Infors ...
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bioRxiv - Biochemistry 2021Quote: Recombinant proteins were labeled with Alexa Fluor™ 647 NHS ester (Thermo Fisher) as described previously.20 In brief ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM Pierce™ recombinant protein A (#21184, Thermo Fisher Scientific, Waltham, USA) 50 nM bovine serum albumin (#A4612 ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Secondary horseradish peroxidase (HRP)-conjugated recombinant A/G protein (Invitrogen, lot number XH346151) diluted 1:20,000 was used for detection and visualized with KPL TMB two-component peroxidase substrate kit (SeraCare ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Cell Biology 2023Quote: ... all iPSCs were cultured in Vitronectin Recombinant Human Protein (VTN-N) (ThermoFisher, A14700) pre-coated 6-well plates with 2 mL TeSR-E8 medium (Life Technologies ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Sybr Safe DNA gel stain and BenchMark™ His-tagged Protein Standard were purchased from Invitrogen. HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech ...
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bioRxiv - Evolutionary Biology 2020Quote: ... His-tagged proteins were eluted from the beads in a disposable polypropylene column (29924, ThermoFisher Scientific) using elution buffer (4 ml wash buffer containing 400 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... media was collected and His tagged proteins were bound using HisPur Ni-NTA Resin (ThermoFisher; 88221) in the presence of Calbiochem’s EDTA-free protease inhibitor cocktail 1:1000 (MilliporeSigma ...
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bioRxiv - Genetics 2019Quote: ... Proteins tagged with the V5 epitope (Hxk2, Reg1) were detected with the Anti-V5 probe (Invitrogen) diluted 1:1,000 ...
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bioRxiv - Microbiology 2023Quote: ... we used PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Genetics 2023Quote: ... Flag-tagged proteins were immunoprecipitated with anti-Flag magnetic agarose (Pierce Anti-DYKDDDDK Magnetic Agarose, ThermoFisher), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... cells were activated by adding 50 ng/mL recombinant mouse IFN gamma (Invitrogen). On day 7 ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with 10 ng/mL (~100 U/mL) recombinant mouse IFNγ (Gibco) for 24 h prior to infection ...
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bioRxiv - Immunology 2023Quote: ... Clec12a-Fc or Fc only fusion proteins were isolated with Pierce™ Classic Magnetic IP/Co-IP Kit (Thermo Fisher Scientific, Catalog number: 88804), quantified with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific Catalog number ...
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bioRxiv - Immunology 2024Quote: ... the cells were probed with donkey anti-mouse secondary antibody tagged with Alexa Fluor 568 (Invitrogen), for 1 h in dark ...
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: Rhodamine-tagged dextran (Invitrogen) was encapsulated into AlgLung and S-AlgLung hydrogels at a concentration of 0.5 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Fab and Fc regions were separated using a Nab Protein A column (Thermo Scientific). Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off ...
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bioRxiv - Synthetic Biology 2023Quote: ... and Fc fusions were purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fc-PRL-13 was purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions with the exception that all buffers were supplemented with 1tab/50mL of cOmplete Protease Inhibitor Cocktail and 10uM Pepstatin A ...
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bioRxiv - Microbiology 2024Quote: Recombinant Bd37 (rBd37) protein was expressed as thioredoxin fusion protein from the pBAD/Thio-TOPO vector (Thermo Fisher Scientific), which encodes thioredoxin and histidine-tag at N-terminus and C-terminus of multi cloning site ...
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bioRxiv - Biochemistry 2021Quote: ... 30 μg of biotinylated protein were mixed with 60 μL of streptavidin agarose resin (Thermo Fisher) and rotated (end-over-end ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... Biotinylated surface proteins were then isolated with neutravidin-coated agarose beads (Thermo Fisher Scientific, Darmstadt Germany). The isolates were boiled at 95 °C for 7 min in the presence (reducing ...
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bioRxiv - Biochemistry 2021Quote: Biotinylated proteins from BioID experiments were purified for proteomic analysis with Dynabeads MyOne Streptavidin C1 (Invitrogen) as previously described [31] ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were dialyzed into bicarbonate buffer and biotinylated using EZ-LinkTM Sulfo-NHS-LC-Biotin (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Purified ACE2 protein was biotinylated using the EZ-Link Sulfo-NHS-Biotin kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Biotinylated SARS-CoV-2 S1 and RBD proteins were combined with fluorescent NeutrAvidin beads (Life Technologies) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... biotinylated-S1B/RBD proteins were captured on high-capacity streptavidin coated 96-well plates (Thermo Scientific) by incubating with 100 µL of 2 µg/mL protein solution in TBS overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were captured on 100 µl of packed high capacity NeutrAvidin sepharose beads (Thermo Fisher) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were isolated by streptavidin pull-down (SA-Pd) using SA-coated magnetic beads (Invitrogen). The biotin-labeled proteins were separated by SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... lysed and subjected to a pull-down assay of biotinylated proteins with neutravidin-agarose (Thermo Scientific) as previously described (Roux et al ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected with 30 min additional incubation with Streptavidin-Alexa 647 (Thermo Fisher Scientific). Membranes were washed four times in PBS and imaged using the Odyssey Imaging System.
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bioRxiv - Microbiology 2023Quote: ... the biotinylated proteins were visualized using SuperSignal Western blot FEMTO substrate (Thermo Scientific, Cat. No. 34094).
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bioRxiv - Microbiology 2023Quote: ... Purified ACE2 protein was biotinylated using the EZ-Link Sulfo-NHS-Biotin kit (Thermo Fisher Scientific) as reported previously 67.
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bioRxiv - Biochemistry 2023Quote: ... Smurf1 HECT NT protein was biotinylated using the No-Weight NHS-PEO4-Biotin kit (Thermo Fisher) due to its compatibility with Super Streptavidin (SSA ...
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bioRxiv - Biochemistry 2024Quote: ... samples were cleaned from endogenously biotinylated proteins using Pierce™ NeutrAvidin™ Agarose beads (Thermofisher, #29200) and precipitated ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected by incubating samples in Alexa Fluor 568 conjugated streptavidin (1:1000; ThermoFisher) diluted in blocking solution for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... and surface proteins were biotinylated with 0.5 mg/mL sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate (ThermoFisher) on ice for 45 minutes with gentle shaking ...
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bioRxiv - Microbiology 2023Quote: Proteins on the surface of Env-expressing HEK293T cells were biotinylated with the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cancer Biology 2019Quote: ... washed and resuspended in FC buffer with goat-anti-mouse Alexa488 (Invitrogen; 1:400) for an additional 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Detection was performed with secondary goat anti-mouse IgG Fc HRP antibody (Invitrogen, A16084) and WesternBright Sirius chemiluminescent HRP conjugate (advansta ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (FCS, Thermo Fisher Scientific), 100 U ml−1 penicillin ...