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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection with purified DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant adenoviruses were produced in HEK293A cells (Invitrogen) and purified by CsCl gradient centrifugation ...
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bioRxiv - Immunology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection with purified DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... 8,000 melanoma cells were seeded into one well of a U-bottomed 96-well plate (Thermo Fisher Scientific, Rochester, NY) to generate the spheroid as described above ...
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bioRxiv - Genomics 2019Quote: ... Nuclear extracts were prepared from actively growing melanoma cells (UACC2331) using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific). Probes were bound to 2 µg nuclear extracts pre-incubated with 1 µg poly d(I-C ...
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bioRxiv - Genomics 2019Quote: ... The resulting constructs were co-transfected with renilla luciferase into melanoma cell lines (UACC903 and UACC502) using Lipofetamine 2000 reagent following the manufacturer’s instructions (Thermo Fisher) in 24-well format ...
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bioRxiv - Cancer Biology 2022Quote: Melanoma cells were maintained in culture medium containing 50 U/ml streptomycin/penicillin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) until they reached 90% confluency ...
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bioRxiv - Microbiology 2022Quote: ... or membrane probed with mouse monoclonal 6x-His tag antibody (4A12E4) (Thermofisher, Waltham, MA) (Supplementary Figure 2).
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bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated in Alexa tagged secondary antibodies (Life Technologies, Invitrogen) prepared in SG-PBS for 1hr at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated in Alexa tagged secondary antibodies (Life Technologies, Invitrogen) prepared in SG-PBS for 1hr at room temperature ...
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bioRxiv - Genetics 2023Quote: The lipophilic molecule BODIPY 493/503 (Invitrogen, D3922) was dissolved in 100% DMSO to 1 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... the adhesion media was removed and replaced with Neurobasal medium (Life Technologies) containing B27 supplement (Life Technologies) ...
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bioRxiv - Neuroscience 2019Quote: ... 50 μm-thick slices were collected on polysine microscope adhesion slides (ThermoFisher) and then incubated in blocking solution (3% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... and then mounted onto Polysine Adhesion Slides (Thermo Fisher Scientific, Waltham, USA). RNA probe hybridization and subsequent washes were performed as described previously (Ansel et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The two halves were placed on SuperFrost Plus adhesion slides (Thermo Scientific) side by side and at 180 degrees to each other to have for both the areas of interest ...
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bioRxiv - Cell Biology 2019Quote: ... The cryosections were placed on glass slides (SuperFrost Plus Adhesion, Fisher Scientific), dried at 37°C and rehydrated using PBS ...
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bioRxiv - Neuroscience 2019Quote: ... thaw-mounted onto SuperFrost Ultra Plus adhesion slides (Thermo Scientific, MA, USA), and stored at −20°C until further processing ...
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bioRxiv - Neuroscience 2021Quote: ... and collected on SuperFrost Plus Adhesion Slides (Thermo Fisher Scientific, Waltham, MA). Sections were spread across multiple slides and wildtype controls were collected next to heterozygous and/or homozygous samples on the same slide to achieve equal conditions during staining and washing steps for all genotypes ...
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bioRxiv - Microbiology 2023Quote: Adhesion to extracellular matrix proteins: MaxiSorp 96-well flat-bottom plates (ThermoFisher) were coated overnight at 4°C under agitation with 1 μg per well ...
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bioRxiv - Neuroscience 2020Quote: ... cells resuspended in 500µl 2% HI-FBS + PBS (ThermoFisher 12484028), and passed through a 40µm nylon mesh cell strainer (FisherScientific 352235) ...
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bioRxiv - Microbiology 2020Quote: ... Whole cell lysates were added to His beads (Thermo Scientific HisPur Ni-NTA Magnetic Beads ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were resuspended in adhesion media (MEM, 10% Horse Serum, 30% glucose, L-glutamine and penicillin/streptomycin) (Life Technologies) and plated in 6-well plates coated with poly-L-lysine 0.1% w/v in water (Brunschwig ...
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bioRxiv - Cancer Biology 2023Quote: ... the transfected cells were trypsinized and clustered in 96-well Nunclon Sphera U-bottom low adhesion plates (Thermo Scientific, 174925) overnight (1000 cells/well) ...
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bioRxiv - Immunology 2022Quote: ... pAc5.1A/V5-His-CBF and pAc5.1A/V5-His-EF-1α were transfected into S2 cells using Cellfectin II Reagent (Invitrogen, USA), respectively ...
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bioRxiv - Cell Biology 2019Quote: Mouse TGF-β 1 recombinant protein was obtained from Affymetrix (#14-8342-62) and used at the concentration of 2 ng/ml unless stated otherwise ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with 10 ng/mL (~100 U/mL) recombinant mouse IFNγ (Gibco) for 24 h prior to infection ...
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bioRxiv - Genetics 2021Quote: ... Epitope-tagged bait proteins were detected with mouse anti-c-myc monoclonal antibody (Invitrogen, MA1-980) and IR-680 conjugated donkey anti-mouse secondary antibody (LI-COR ...
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bioRxiv - Bioengineering 2023Quote: Rhodamine-tagged dextran (Invitrogen) was encapsulated into AlgLung and S-AlgLung hydrogels at a concentration of 0.5 mg/ml ...
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bioRxiv - Biophysics 2021Quote: ... Human Melanoma A-375 cells (Cat #: CRL-1619) were obtained from ATCC and cultured in DMEM supplemented with 10% FBS (Life Technologies), 1x GlutaMAX (Life Technologies) ...
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bioRxiv - Genetics 2021Quote: ... into the melanoma cell line UACC 903 and multiple human melanocyte cultures (c23, c197 and c87) using Lipofectamine 2000 reagent (Thermo Fisher) for UACC903 or electroporation with the Lonza Amaxa P2 kit and the CA-137 protocol on the 4D-Nucleofector system for primary melanocytes ...
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bioRxiv - Immunology 2023Quote: ... injection of 2.5 × 105 B16-OVA melanoma cells (gift of Hyam Levitsky) cultured in vitro under OVA selection media containing 400 μg/ml G418 (Life technologies) or 5 × 105 B16-OVA-CD19 (kindly provided by Anjana Rao(24)) ...
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-6x-His epitope tag monoclonal antibody (Thermo Fisher Scientific, Cat. No. MA1-21315); LI-COR IRDye 800CW goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Immunology 2021Quote: ... Anti-6x(HIS) anti-mouse antibody was used to recognize VHH (4A12E6: Invitrogen Rockford, USA) followed by probing with anti-mouse IgG raised in goat (A3562 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The PVDF membrane was then incubated with anti-his mouse primary antibody (Thermo Scientific Pierce) in TBS-T (1:5000 ...
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bioRxiv - Biophysics 2019Quote: ... the final dye to protein ratio was typically 5-6 dye molecules per protein molecule as measured with a Nanodrop spectrophotometer (Thermo Fisher Scientific). Cells in suspension culture dishes were labeled with 0.5 nM fluorescent IgE for typically 12-20 hours before imaging.
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bioRxiv - Biochemistry 2022Quote: Recombinant human Dynactin was expressed using Expi293F cells (Invitrogen) and Expi293 expression medium (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (Gibco, #A14527) following the manufacturer’s directions ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were expressed in Expi293F cells (Life technologies). Full-length spike proteins were cloned into the pCAGGS mammalian expression vector as previously described79,80 ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... the cryosections were mounted on SuperFrost Plus Adhesion slides (Thermo Fisher Scientific, 12625336) and stored at −80°C ...
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bioRxiv - Neuroscience 2019Quote: Brain cryosections were mounted on superfrost plus adhesion slides (Thermo Fisher, Waltham, MA) and stored at −80 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... The mixture was transferred onto a Superfrost Ultra Plus Adhesion Slide (ThermoFisher Scientific) and incubated for 1 h at 37°C ...