Labshake search
Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Recombinant Mouse Epcam His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
-
bioRxiv - Biochemistry 2022Quote: ... His6- tagged SPOC domains were purified by affinity chromatography using HisTrap HP column (Cytiva) or His-Pur Ni-NTA resin (Thermo Scientific) equilibrated in 25 mM Tris-Cl pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... the N-terminal His-tagged protein was purified in a single step protocol using HisPur Ni-NTA resin (Thermo Fisher Scientific), equilibrated at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... The 6X His tagged extracellular domain of hPD-L1 WT or 2HA proteins were expressed in the ExpiCHO cell system (ThermoFisher Scientific) and purified by the Ni-NTA agarose (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... mammalian expression constructs carrying C-terminal 6X-His tagged gene of interest (HpARI1-3, or ST2 ectodomain) were transfected individually into Expi293F cells (Thermo Fisher) using the Expifectamine transfection kit (Thermo Fisher) ...
-
bioRxiv - Immunology 2021Quote: ... cells were pretreated with mouse recombinant IFN-γ (BMS326, Invitrogen) at 6.25ng/mL for overnight and followed by infection with Mycobacterium tuberculosis ...
-
bioRxiv - Cancer Biology 2022Quote: ... 50 ng/ml recombinant mouse EGF (Thermo Fisher Scientific, PMG8041), 1 ug/ml recombinant human R-spondin1 (R&D ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 10 ng/mL recombinant mouse interleukin-3 (Gibco PMC0031).
-
bioRxiv - Cancer Biology 2024Quote: ... supplemented with 50 ng/ml mouse recombinant EGF (Invitrogen, PMG8043). The organoids were trypsinized with 0.05% Trypsin-EDTA (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... EpCAM-APC (1:300, ThermoFisher, 17-5791-82). 7AAD (ThermoFisher ...
-
bioRxiv - Cell Biology 2022Quote: ... and EpCAM (anti-human CD326 -PE, Affymetrix eBioscience) antibodies respectively for 30 min in the dark at 4°C following manufacturer instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... EpCAM-APC (1:63; Invitrogen 17-5791-82); CD31-450 (1:170 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and anti-EpCAM (PE-Texas Red, Thermo Fisher) antibodies and analyzed using ImageStream analysis ...
-
bioRxiv - Genomics 2022Quote: ... a 1:100 dilution of Anti-mouse EpCAM antibody conjugated to eFluor450 (Thermo Fisher Scientific, Cat#: 48-5791-82), and 1:100 Anti-EphrinB2 unconjugated antibody (BD Biosciences ...
-
bioRxiv - Genomics 2022Quote: ... and 1:100 dilution of Anti-mouse EpCAM antibody conjugated to eFluor450 (Thermo Fisher Scientific, Cat#: 48-5791-82). Liver epithelial cells were isolated using the following cell surface marker profile ...
-
bioRxiv - Microbiology 2023Quote: ... then incubated with Alexa-488 tagged secondary antibody (goat anti-mouse IgG, Invitrogen) at a dilution of 1:500 ...
-
bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
-
bioRxiv - Microbiology 2022Quote: ... monoclonal mouse anti-6x-His antibodies (Epitope-tag-clone: HIS.H8, Thermo Fisher) were employed ...
-
bioRxiv - Microbiology 2022Quote: ... The following antibodies were used: mouse anti-His (MA1-21315, Thermo Scientific), goat anti-mouse HRP (405306 ...
-
bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
-
bioRxiv - Cell Biology 2021Quote: ... reaction mixes were prepared using 2.5 μg recombinant His-ACBD5 with or without the addition of 0.3 mM ice-cold ATP (Thermo Fisher) and 0.1 ug GST-GSK3β (Abcam) ...
-
bioRxiv - Biophysics 2022Quote: The gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al. ...
-
bioRxiv - Biophysics 2022Quote: ... the gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). For determination of λmax of V2HeR3 WT and mutants ...
-
bioRxiv - Microbiology 2021Quote: ... Purification of His-tagged Cg1978 was performed by applying the supernatant to an equilibrated 2 ml Ni-NTA column (Invitrogen, Kalifornien, USA). After washing with 30 ml TNI20 buffer ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: His-tagged relaxins were expressed as secreted proteins in Expi293F cells containing a stably integrated tetracycline repressor (Expi293F tetR, Thermo Fisher Scientific) grown in Expi293 media (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... Expi293F™ cells were transiently transfected with His-tagged gB ectodomain plasmids for five days and later harvested with Nickel-NTA resin (Thermo Fisher Scientific).
-
bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...
-
bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
-
bioRxiv - Genetics 2021Quote: ... 50 ng/ml recombinant mouse epidermal growth factor (EGF; Gibco, PMG8041), 100 ng/ml recombinant murine Noggin (PeproTech ...
-
bioRxiv - Cell Biology 2022Quote: ... 10 ng/ml mouse recombinant LIF (ES cell-tested) (Gibco #A35935). Upon reaching confluence ...
-
bioRxiv - Genetics 2023Quote: ... 50 ng/ml recombinant mouse epidermal growth factor (EGF; Gibco, PMG8041), 100 ng/ml recombinant murine Noggin (PeproTech ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1% penicillin/streptomycin and 50ng/ml recombinant mouse EGF (Life Technologies) was used for culturing ApcKO colon organoids ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin/streptomycin and 50ng/ml recombinant mouse EGF (Life Technologies) was used for culturing colon organoids ...
-
bioRxiv - Developmental Biology 2020Quote: ... For FACS with EpCAM-APC (Invitrogen, 17-5791-80), cells were diluted to less than 1 ×106 cells/mL in PBS with 3% FBS and incubated in 1:100 EpCAM-APC (Invitrogen ...
-
bioRxiv - Bioengineering 2022Quote: ... Two microliters of biotinylated anti-EpCAM (Thermo Fisher Scientific) was added ...
-
bioRxiv - Cell Biology 2023Quote: ... The EPCAM- cells were cultured in DMEM (Gibco, 41965039) for 1~2 passages and stored at −80°C for further using.
-
bioRxiv - Cancer Biology 2024Quote: ... Sections were stained with primary antibodies to EpCAM (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... Anti-EpCAM antibody was purchased from Invitrogen (MA, USA) and anti-CD63 was purchased from BD Biosciences (NJ ...
-
bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
-
bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
-
bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
-
bioRxiv - Cell Biology 2020Quote: Recombinant 6XHis-tagged TbVps24 (N-terminus tag) was cloned using the pTrcHis TOPO TA expression system (Thermo Fisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-mouse IgG (H+L) antibody (Invitrogen), before being acquired with BD FACSCanto II (BD Biosciences) ...
-
bioRxiv - Microbiology 2020Quote: ... SV5-tagged proteins were detected using mouse anti-SV5 mAb (1:5000, Life Technologies); HA-tagged proteins were detected by anti-HA mAb (clone HA-7 ...
-
bioRxiv - Neuroscience 2023Quote: ... HA-tagged scFvs were detected using mouse mAb 2-2.2.14 (ThermoFisher Catalog# P126183, RRID:AB_10978021). The following day sections were washed 4 x 5 min with vehicle ...
-
bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
-
bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
-
bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
-
bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
-
bioRxiv - Immunology 2022Quote: ... The plate was coated with 2 µg/mL mouse anti-His antibody (Invitrogen cat ...