Labshake search
Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Recombinant Mouse Cxcl2 His & SUMO tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
-
bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide, Applied Biosystems) and incubated at room temperature for 20 minutes ...
-
bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
-
bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
-
bioRxiv - Cell Biology 2020Quote: ... fluorescently tagged BODIPY® (BODIPY® FL C12; Invitrogen D3822) stock was dried down completely in a speed vacuum and re-suspended in canola oil to a final concentration of 1mg/ml ...
-
bioRxiv - Cancer Biology 2021Quote: ... and were incubated and tagged with CFSE (Invitrogen CellTrace, C34570) at 1 µL/mL for 20 min ...
-
bioRxiv - Cell Biology 2021Quote: ... These LAP tagged contructs were co-tansfected with pOG44 (Invitrogen) into Flp-In HeLa T-REx cells ...
-
bioRxiv - Developmental Biology 2019Quote: ... FLAG/HA-tagged AKAP2 was cloned using Gateway technology (Invitrogen) by PCR amplification of the AKAP2 cDNA using attB-flanked BP primers (see Table S2) ...
-
bioRxiv - Molecular Biology 2020Quote: ... for YFP-tagged α globin and Neon electroporation system (Invitrogen, according to manufacturer’s instructions ...
-
HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: Flag tagged Nef gene was constructed into pLenti vector (Invitrogen). pLenti-Nef plasmids or pLenti empty vectors were co-transfected with pLP1 ...
-
bioRxiv - Physiology 2020Quote: ... appropriate species-specific fluorescently tagged secondary antibodies (Invitrogen, Carlsbad, CA) were used before mounting with DAPI (Vector Laboratories ...
-
bioRxiv - Cell Biology 2022Quote: Myc-tagged p57 and p57CK- in pcDNA3.1+ Hygro vector (Invitrogen) were described previously [5] ...
-
bioRxiv - Neuroscience 2023Quote: ... and secondary AlexaFluor 488 tagged anti-Rabbit (Life Technologies; A21206). Sections were mounted using Prolong Gold Anti-Fade Reagent (Life Technologies ...
-
bioRxiv - Bioengineering 2023Quote: ... and a 546-nm fluorescently tagged Protein A (ThermoFisher, P11049) as our linking protein ...
-
bioRxiv - Cancer Biology 2023Quote: ... incubated with appropriate fluorescent-tagged secondary antibodies (1:250, Invitrogen) for two hours at room temperature ...
-
bioRxiv - Biophysics 2023Quote: ... and a Cy5-tagged secondary antibody (1:2000, Thermo Fisher Scientific Inc. ...
-
bioRxiv - Cell Biology 2024Quote: ... APEX2-tagged hiPSCs were washed with DPBS (Thermo Fisher, 14190136) and then incubated in DPBS containing 0.5 mM biotin-phenol (Iris Biotech ...
-
bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were visualized using Pierce ECL (Thermo Scientific) after treatment with an HRP-conjugated primary antibody directed against the FLAG epitope (Millipore Sigma) ...
-
bioRxiv - Biophysics 2021Quote: The cDNA segment encoding the C1B domain of protein kinase C (PKC) δ isoenzyme from Rattus norvegicus (amino acids 229-281) was sub-cloned into pET SUMO expression vector (Invitrogen). The His6-SUMO-C1Bδ fusion protein was expressed in Escherichia coli BL21(DE3 ...
-
bioRxiv - Microbiology 2020Quote: ... subtilis 168 as the template and appropriate nucleotides that attached BsaI and XhoI restriction sites to the fragments and cloned between the BsaI and XhoI sites of the expression vector pET-SUMO (Invitrogen, Germany). The resulting plasmid was pGP2972 ...
-
bioRxiv - Molecular Biology 2021Quote: ... PICS-1EBM (residues 180-200) and a TOST-1 fragment (residues 34-54) were cloned into pET28-SUMO vector (Invitrogen™).
-
bioRxiv - Cell Biology 2023Quote: ... HeLa S3 cervical adenocarcinoma cells and HeLa 6xHis-SUMO cells (kind gift from Ronald T. Hay, University of Dundee, UK) were cultured in DMEM medium (Gibco, Life Technologies) supplemented with 10% FBS (Capricorn Scientific) ...
-
bioRxiv - Bioengineering 2023Quote: ... mCXCL10-LPETG SUMO fusion proteins were purified by Ni-NTA pulldown and then treated overnight with Endotoxin Removal columns (Thermofisher, 88274). Cleavage of mCXCL10-LPETG from the greater SUMO fusion proteins was carried out via ULP1 digestion (Thermofisher ...
-
bioRxiv - Biophysics 2023Quote: ... The His14-SUMO tag was separated from cys-ubiquitin by passing the dialyzed eluate over HisPur Ni-NTA Resin (Thermo Scientific). For labeling ...
-
bioRxiv - Microbiology 2023Quote: ... we cloned the region of the rocR gene that corresponds to amino acids 141 to 400 into pET-SUMO (Invitrogen, Germany). For this purpose ...
-
bioRxiv - Biophysics 2023Quote: Plasmids encoding and expressing M with an N-terminal 6xHis tag and with and without SUMO tag fusions were designed in VectorNTI software (ThermoFisher Scientific) and synthesized de novo by GENEWIZ/Azenta Gene Synthesis service ...
-
bioRxiv - Microbiology 2024Quote: ... subtilis 168 as the template and appropriate nucleotides that attached BsaI and XhoI restriction sites to the fragments and cloned between the BsaI and XhoI sites of the expression vector pET-SUMO (Invitrogen, Germany). The resulting plasmid was pGP3630 ...
-
bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
-
bioRxiv - Biochemistry 2022Quote: Recombinant hItln1 and MBL were characterized by western blotting using monoclonal mouse anti-MBL2 antibody (3E7; ThermoFisher Scientific MA1-40145; 1:50) and polyclonal rabbit anti-hItln1 antibody (Proteintech 11770-1-AP ...
-
bioRxiv - Microbiology 2019Quote: ... His-FruB or His-HPr in the cell extract was purified using HisPurTM-Ni-NTA Superflow Agarose (from Thermo Scientific, Cat # 25214) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
-
bioRxiv - Genetics 2020Quote: ... pAc5.1/V5-His version C (V411020, Thermo Fisher Scientific). In order to test the transferring ability of gypsy ...
-
bioRxiv - Developmental Biology 2021Quote: ... and inserted into pMT/V5-His A (Thermo Scientific) using XhoI and SpeI target sites ...
-
bioRxiv - Cell Biology 2022Quote: pAc5.1/V5-His vector (Thermo Fisher Scientific, Cat #V411020) was used to construct plasmids for expressing proteins or miRNAs in Drosophila S2 cells ...
-
bioRxiv - Molecular Biology 2019Quote: ... and Ion PGM Hi-Q Sequencing Kit (Life Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... with 10% heat-inactivated FBS (HI-FBS; Life Technologies)) ...
-
bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
-
bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
-
bioRxiv - Neuroscience 2020Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
-
bioRxiv - Microbiology 2020Quote: ... and 9.9 μl of Hi-Di formamide (Applied Biosystems). These were then incubated for 5 min at 95°C and placed immediately on ice ...
-
bioRxiv - Immunology 2020Quote: ... and ligated into pcDNA3.1D/V5-His-TOPO vector (Invitrogen). Pseudoviruses were prepared by co-transfecting 1.25 µg of HIV-1 envelope containing plasmid with 2.5 µg of an envelope deficient HIV-1 backbone (PSG3Δenv ...
-
bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
-
bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
-
bioRxiv - Molecular Biology 2022Quote: ... Pre-washed 25 µl His-Tag Dynabeads (ThermoFisher Scientific) were added ...
-
bioRxiv - Neuroscience 2019Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
-
bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
-
bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
-
bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
-
bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...