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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Recombinant Mouse CD8a Antigen Alpha Chain His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... sorted cells were cultured on irradiated OP9DLL1 monolayers in Alpha-MEM (Alpha-MEM, ThermoFisher, 12000063) supplemented with 20% FBS (HyClone) ...
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Immunology 2021Quote: Antibody heavy chain and light chain constructs were transiently expressed with the Expi293 Expression System (ThermoFisher). HC and LC plasmids were cotransfected at a 1:2.5 ratio with transfection reagent FectoPRO (Polyplus ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-6x-His epitope tag monoclonal antibody (Thermo Fisher Scientific, Cat. No. MA1-21315); LI-COR IRDye 800CW goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Immunology 2021Quote: ... Anti-6x(HIS) anti-mouse antibody was used to recognize VHH (4A12E6: Invitrogen Rockford, USA) followed by probing with anti-mouse IgG raised in goat (A3562 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The PVDF membrane was then incubated with anti-his mouse primary antibody (Thermo Scientific Pierce) in TBS-T (1:5000 ...
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bioRxiv - Cell Biology 2021Quote: The primary antibodies used were mouse anti-clathrin light chain (AB CON.1, MA5-11860, Thermo Fisher Scientific), mouse anti-clathrin heavy chain (AB X-22 ...
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bioRxiv - Immunology 2022Quote: ... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
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bioRxiv - Physiology 2022Quote: ... Targeted antigen retrieval was performed by boiling sections in 1x IHC antigen retrieval solution (Invitrogen) for 15-20 mins ...
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bioRxiv - Immunology 2022Quote: ... anti-CD8a-PE-Cy7 (Invitrogen 25-0081-82: Clone: 53-6.7, eBioscience), anti-CD62L-PE (Invitrogen 12-0621-82 ...
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bioRxiv - Bioengineering 2022Quote: ... and Myosin Heavy Chain (ThermoFisher #Bt03273061). Reactions were performed according to the manufacturer’s instructions on a Bio-Rad CFX96 Real Time System thermocycler ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit or mouse IgG (Thermo Fisher Scientific) for 6h at 4°C in the following day ...
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bioRxiv - Synthetic Biology 2021Quote: ... alpha-pinene (Acros Organics, CAT#: 131270050); limonene (Tokyo Chemical Industry ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-Tubulin (MS581P1) from Thermo Fisher, SEC23B (GTX82432 ...
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bioRxiv - Developmental Biology 2019Quote: ... and TNF-alpha (100ng/ml, Gibco) were used ...
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bioRxiv - Developmental Biology 2020Quote: ... 1:3000 alpha-bungarotoxin 488 (Invitrogen), 1:1000 rabbit anti-Syntaxin1 (gift of Thomas Südhof ...
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bioRxiv - Immunology 2020Quote: ... and Interferon alpha (Invitrogen, Cat. BMS6027TWO). Plates were read using an Epoch BioTek Gen5 microplate reader at 450nm ...
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bioRxiv - Microbiology 2022Quote: ... CD8-alpha Pacific Orange (5H10, ThermoFisher), CD11b PerCP-Cy5.5 (M1/70) ...
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bioRxiv - Systems Biology 2022Quote: ... TNFα (tumor necrosis factor-alpha) (ThermoFisher), PGE2 (prostaglandin E2 ...
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bioRxiv - Physiology 2024Quote: ... and alpha-smooth muscle actin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Hexahistidine-tagged proteins were then detected using mouse anti-6xHis tag antibody (Ma1-135, Invitrogen, Karlsruhe, Germany) as primary antibody and signals revealed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (ab6789 ...
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bioRxiv - Neuroscience 2024Quote: ... recombinant polyclonal goat anti-mouse coupled to Alexa Fluor 488 (Thermo Fisher, A-28175), and polyclonal goat anti-mouse coupled to Alexa Fluor 568 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Cytochalasin D (C8273, Neta) along with recombinant Mouse TNF-α (# RMTNFAI, Thermo Fisher Scientific) and human TNF-α (16769S ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse SGK1.1 (wild type and constitutively active mutant S515D) cloned into pcDNA3.1/V5-His-TOPO (Invitrogen) were kind gifts from Dr ...
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bioRxiv - Immunology 2023Quote: ... or control antigen PE (Thermo Fisher) were mixed with adjuvants per manufacturer’s protocols and added to cells immediately after plating ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
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bioRxiv - Biophysics 2019Quote: ... FLAG-tagged hZP3 and HA-tagged hZP4 were synthesized (GenScript; GeneArt/Thermo Fisher Scientific) and subcloned into pHLsec329 ...
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bioRxiv - Neuroscience 2020Quote: Cytokines released by primary microglia in the culture medium were quantified by ELISA using the following commercial kits according to manufacturer’s instruction: Mouse TNF alpha Uncoated ELISA kit (88-7324-22, Invitrogen), Rat IL-6 DuoSet ELISA (DY506 ...
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bioRxiv - Bioengineering 2021Quote: ... cells were grown in FreeStyle 293 expression medium and first co-transfected with H-chain- and L-chain-containing pcDNA3.1(+) plasmids using 293fectin according to the manufacturer’s protocol (Invitrogen). Stable transfectants producing either IgM617-HL or IgM012-HL were generated using cultivation in medium supplemented with 400 μg/ml G418 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... heavy chain plasmid DNA and light chain plasmid DNA were diluted in 1.5ml Opti-Plex Complexation Buffer (Invitrogen) before mixing with 80µl ExpiFectamine 293 Reagent (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Four hundred micrograms of heavy chain and light chain plasmid were complexed with Expifectamine (ThermoFisher Cat No. A14526) and added to the Expi293 cells ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse monoclonal antibodies targeting cell surface antigens included anti-GL7 (FITC, PE, eFluor660; clone GL 7; Thermo Fisher), anti-B220 (PE-Cy7 ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Molecular Biology 2022Quote: ... anti-Clathrin Heavy Chain (1:1,000; Invitrogen); anti-goat-conjugated Alexa-488 (1:500 ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and Myosin Heavy Chain (MHC) (ThermoFisher #Bt03273061). Reactions were performed using 2 uL of the prepared cDNA ...
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bioRxiv - Neuroscience 2019Quote: ... Rabbit anti-GFP tagged FITC (1:500) and mouse anti-Gal4 (1:200) were acquired from Thermo Scientific. Dissected larvae were mounted in Vectashield (Vector Labs ...
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bioRxiv - Microbiology 2024Quote: ... goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor™ 488 tagged (Invitrogen #A-11001) was used ...
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bioRxiv - Microbiology 2021Quote: ... 10% HI FBS (Gibco), 1X ultraglutamine (Lonza) ...
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bioRxiv - Microbiology 2021Quote: ... His (ThermoFisher, MA1-21315), FLAG (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... HI FBS (2273356P, Gibco). HEPES (54457 ...
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bioRxiv - Immunology 2020Quote: ... or anti-His (Invitrogen) for recombinant ZVrecE80 antigen in 0.1M carbonate buffer pH 9.6 ...
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bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
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bioRxiv - Microbiology 2022Quote: ... anti-penta HIS (Invitrogen) and anti-HSP70 (26 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...