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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Recombinant Mouse CD33 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Protein concentration of recombinant SIRT5 was subsequently determined by Bradford assay (#23200, Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... the recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as previously detailed (56 ...
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bioRxiv - Microbiology 2024Quote: Recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: The recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as reported previously (25 ...
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bioRxiv - Immunology 2024Quote: ... recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... The positive control consisted of 100 – 500 ng recombinant Protein A (ThermoFisher Scientific, Santa Clara, CA). The negative control consisted of ~2 μg wt-TMV from purified N ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified cell supernatant containing recombinant mAb was passed over Protein A Agarose resin (Thermo Fisher Scientific). Protein A resin was extensively washed with 25 mM Phosphate pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant Cripto protein or transfected with Cripto expression plasmid (pcCripto) using Lipofectamine2000 (Life Technologies, Carlsbad, USA) according to the manufacturer`s instructions ...
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bioRxiv - Pathology 2022Quote: TRP120 and TRX recombinant proteins were desalted using Zeba spin desalting columns (Thermo Fisher Scientific, MA) as indicated by the manufacturer protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 µL of the cleared lysates were added with 20 µL Recombinant Protein G Agarose (Invitrogen) and rotated for 30 min at 4 °C to preclear ...
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bioRxiv - Microbiology 2021Quote: ... we conjugated recombinant RBD proteins with Alexa Fluor 488 or Alexa Fluor 594 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Quantification of the recombinant proteins was performed by Pierce™ BCA Assay (Thermo Fisher Scientific 23225) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
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bioRxiv - Microbiology 2022Quote: ... we conjugated recombinant RBD proteins with Alexa Fluor 488 or Alexa Fluor 647 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The Pbc43opt recombinant protein was extracted from inclusion bodies using the Inclusion Body Solubilization Reagent (ThermoFisher). The solubilized protein was also purified using TALON® metal affinity resin ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... cell lysate supernatants were mixed with antibodies and recombinant protein G-Sepharose 4B beads (Thermo Fisher) at 4°C for overnight ...
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bioRxiv - Plant Biology 2020Quote: ... Serum was used for antibody affinity purification to the STS1 recombinant protein using magnetic beads (Invitrogen). Validation of antibody was carried out by immunoblot with total protein extract from sts1 complemented lines and sts1 mutant as a negative control ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to red fluorescent Neutravidin beads (Life Technologies). Antibodies were diluted to 5 μg/mL in RPMI-1640 ...
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bioRxiv - Biochemistry 2022Quote: ... All recombinant proteins were examined by SDS-PAGE along with Pierce BSA mass standards (Thermo Fisher), and their concentrations were calculated from infrared scanning of Coomassie-stained gels (Odyssey Fc Imaging System ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guideline 66 ...
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bioRxiv - Neuroscience 2022Quote: ... and the recombinant proteins were purified using HisPur Cobalt Superflow Agarose (Thermo Scientific, Waltham, MA, USA), dialyzed against 1× phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant tau was labeled using an Alexa Fluor 647 Protein Labeling Kit (Cat No. A20173, Invitrogen) with 500 mg of recombinant protein input at a concentration of 1 mg/mL according to the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Recombinant Omicron RBD proteins from variants BA.2 and BA.4.5 were obtained from Fisher Scientific. To determine the specificity of mAbs ...
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bioRxiv - Cancer Biology 2023Quote: ... containing 150 ng/µl sgRNA and 500 ng/ul recombinant TrueCut Cas9 protein (Thermo Fisher, A36496) using Invitrogen Neon Electroporator with 2 pulses of 1200 V ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant Bd37 and Venus (control) proteins were purified by using ProBond Purification system (Thermo Fisher Scientific) according to the manufacture’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... recombinant Cas9 protein (100 ng/μl; GeneArt Platinum Cas9 Nuclease, Thermo Fisher Scientific, Waltham, MA, USA), and ssODNs (400 ng/μl ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse α-TATA Binding Protein (ThermoFisher 49-1036), Goat α-mouse:HRP (Novus Biologicals NBP2-31347H) ...
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bioRxiv - Neuroscience 2023Quote: ... anti-green fluorescent protein mouse IgG2a(Invitrogen 1037264)GFP monoclonal Antibody(clontech632375 ...
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bioRxiv - Neuroscience 2023Quote: ... anti-green fluorescent protein mouse IgG1(Invitrogen A11121); anti-green fluorescent protein mouse IgG2a(Invitrogen 1037264)GFP monoclonal Antibody(clontech632375 ...
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bioRxiv - Microbiology 2022Quote: ... BMDMs and MEFs were stimulated with 10 ng/mL (~100 U/mL) recombinant mouse IFNγ (Gibco) for 24 h prior to infection or left unstimulated ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Biochemistry 2023Quote: ... The molecular weight of the recombinant proteins was estimated using Invitrogen™ BenchMark™ Pre-stained Protein Ladder (Fisher Scientific, Waltham, Massachusetts, USA). Eluted proteins were concentrated using Vivaspin® 10,000 MWCO centrifugal filter units (Sartorius ...
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bioRxiv - Microbiology 2023Quote: ... Purity and concentration of the recombinant proteins was assessed by SDS-PAGE and the Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Gel filtration was used to recover the recombinant protein fraction according to its expected size on an AKTA Ettan LC FPLC System (Amersham Pharmacy Biotech ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and 50μl Pierce recombinant protein A/G with peroxidase (Thermo Fisher catalog no: 32490) added at 1:10,000 in PBS-T with 1% milk as a secondary and incubated 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Plant Biology 2021Quote: For recombinant protein expression in Pichia pastoris GS115 (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs of IF1 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant forms of SyAM in culture supernatant were isolated by protein A affinity resins (MabCapture, ThermoFisher Scientific) using Pierce gentle Ag/Ab elution buffer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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bioRxiv - Immunology 2022Quote: Recombinant gH/gL proteins were biotinylated using the EZ-Link NHS-PEG4-Biotin Kit (ThermoFisher, catalog #21330) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Microbiology 2020Quote: The recombinant proteins were purified using nickel sepharose beads (Ni-NTA, Thermo Fisher Scientific, Waltham, MA, USA). The lysate supernatant from the 50 ml culture was mixed with 300 μl Ni-NTA beads and incubated overnight at 4° in the lysis buffer supplemented with 5 mM imidazole ...
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Pathology 2020Quote: ... A 1ug/mL mixture of 50μL per well containing recombinant protein in carbonate buffer (0.1M Na2CO3 0.1M NaHCO3 pH 9.3) was used to coat MaxiSorp (ThermoFisher) 96-well plates overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.