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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Recombinant Human TNFRSF18 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Microbiology 2024Quote: ... Proteins fused with Fc were captured using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), eluted with pH 3.0 glycine (100 mM in H2O) ...
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated proteins were pulled down with Pierce High Capacity Streptavidin Agarose (20357; ThermoFisher) and were separated by SDS-PAGE and visualized using a SilverXpress silver stain kit (LC6100 ...
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bioRxiv - Neuroscience 2019Quote: ... Immunoprecipitation (IP) of transcripts was achieved via biotinylated-protein L (ThermoFisher Scientific 29997)-coated streptavidin magnetic beads (ThermoFisher Scientific 65602 ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were purified using 260μl MyOne Streptavidin T1 beads (Novex Life Technologies) per 5mg total protein as described previously (22) ...
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bioRxiv - Microbiology 2020Quote: ... S1 proteins were pre-biotinylated using EZ-Link NHS-PEG4-Biotin (ThermoFisher Scientific). Following 20 minutes of pre-hydration of SA biosensors and 1 minute of sensor check ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated protein was purified using a Zeba spin desalting column (Thermo Fisher Scientific). ACE2-Fc was then diluted to 20-83.3 mg/mL in PBS and loaded onto streptavidin biosensors (Forte Bio) ...
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bioRxiv - Immunology 2020Quote: ... was biotinylated using a Fluoreporter Mini-biotin XX protein labeling kit (Life Technologies) and detected using streptavidin-Qdot 585 ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated-CoV proteins were captured on streptavidin coated 96-well plates (Thermo Scientific) by incubating with 100 µL of 2 µg/mL protein solution in TBS overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... L-AHA labelled proteins were biotinylated using PEG4 carboxamide-Propargyl Biotin (ThermoFisher, B10185) and biotin-labelled proteins were isolated by immunoprecipitation using streptavidin magnetic beads (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were captured using Pierce Streptavidin Magnetic Beads (Thermo Fisher Scientific, 88817) at 4°C on a rotator for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected with streptavidin coupled to Alexa Fluor 594 (Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... The biotinylated proteins were tetramerized by adding APC-conjugated streptavidin (Thermo Fisher Scientific) at an allergen ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were enriched using magnetic streptavidin beads (Dynabeads M-280, Invitrogen, USA). The beads were washed with a series of SDS buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Biotinylated proteins were detected with NeutrAvidin DyLight 800 (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2021Quote: ... and the biotinylated secondary anti-human IgG (ref. B1140-1ML, MilliporeSigma, St. Louis, USA)] and the anti-human IgE (ref. A18803, Invitrogen, Waltham, USA) antibodies were added at 1:1250 and 1:100 dilutions ...
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bioRxiv - Immunology 2019Quote: ... KIR2DL1*001-Fc, and KIR2DS4*001-Fc (R&D, 1844-KR, 1847-KR) were conjugated to protein-A Alexa Flour 647 (Invitrogen) by overnight incubation at 9:1 (molar ...
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bioRxiv - Genetics 2022Quote: 3T3-L1 cells expressing GFP-tagged human CIDEC were incubated with MitoTracker (#M7512; Thermo Fisher Scientific) before fixation ...
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bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant protein was transiently expressed in Expi293™ (ThermoFisher Scientific, UK) and purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant CEACAM-His proteins were expressed in Expi293F cells (Life Technologies) and purified by affinity chromatography (ÄKTA Pure ...
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bioRxiv - Immunology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen) and purified by nickel affinity chromatography ...
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bioRxiv - Cancer Biology 2021Quote: ... 40 μL/sample of recombinant Protein-G-Sepharose-4B beads (ThermoFisher) was washed thrice with IP buffer and added to each sample for equilibration on a rotator for 4h ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced using the Expi293F cells (Thermo Fisher Scientific) by transfecting 200×106 of these cells with purified DNA using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant sACE2 protein was purified by nickel affinity columns (Invitrogen) and ACE2-Fc was purified using Protein A affinity column (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were expressed in Escherichia coli BL21 DE3 (Invitrogen), in LB medium ...
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bioRxiv - Immunology 2024Quote: ... used for recombinant protein production were maintained in FreeStyle (ThermoFisher, #12338026) or Expi293 media (ThermoFisher ...
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bioRxiv - Synthetic Biology 2023Quote: All recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transient transfection ...
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bioRxiv - Immunology 2024Quote: ... Recombinant proteins were diluted in DPBS (Life technologies cat. # 14040-182) (tetNA was diluted to 0.5 µg/ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated antibody specific for the Fc region of human IgG (Invitrogen) was used as secondary antibody to detect antibody binding in ELISA experiments.
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled anti-human IgG Fc antibody (Catalog# 31413, Thermo Fisher) was added to each well and incubated for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated Abs specific for the Fc region of human IgG (Invitrogen) were used as secondary antibodies to detect antibody binding in ELISA experiments ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Protease susceptibility measurements were performed in biological replicates (n = 2 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Titrations were performed in biological replicates (cultures from independent colonies grown on separate days ...
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bioRxiv - Immunology 2020Quote: ... followed by addition to the Fc-chimeric human ACE2-coated MaxiSORP ELISA plate (Nunc) for an hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Human JAGGED1-Fc (Martin et al., 2023) was transfected into Expi293F cells (ThermoFisher, A14527) using FectroPro (Polyplus ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human Fc chip was regenerated to baseline using IgG Elution Buffer (Thermo Scientific) and the process was repeated for all humAbs in the panel ...
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bioRxiv - Immunology 2020Quote: Purified recombinant S2P or RBD were biotinylated at a theoretical 1:1 ratio using the Easylink NHS-biotin kit (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: Recombinant protein concentration was quantified through BCA protein assay kit (Thermo Fisher Scientific, Buenos Aires, Argentina) on a BioRad 3350 Microplate Reader using BSA as standard ...
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bioRxiv - Immunology 2022Quote: ... The concentrations of the purified recombinant proteins were determined by BCA protein assay kit (Thermo Scientific) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... The concentrations of the purified recombinant proteins were determined by the BCA protein assay kit (ThermoFisher) and separated by reducing SDS-PAGE and visualized by silver staining ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RBD-hFc proteins were captured by Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), washed by wash buffer (100 mM Tris/HCl ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).