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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Recombinant Human SERPINC1 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... and recombinant human FGF2 (Invitrogen) at 20 ng/ml ...
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bioRxiv - Bioengineering 2022Quote: ... supernatants were harvested and filtered with a 0.22 µm membrane.The His-tagged proteins were purified with the HisPur Ni- NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Bioengineering 2021Quote: ... supernatants were harvested and filtered with a 0.22 μm membrane.The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Cell Biology 2022Quote: His- and GST-tagged CFAP418 proteins were expressed in One Shot™ BL21 Star™ (DE3) cells (ThermoFisher, Waltham, MA) and purified using HisPur™ Ni-NTA resin and Pierce™ Glutathione Superflow Agarose ...
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bioRxiv - Cell Biology 2019Quote: ... Infected Sf9 cells were grown in spinner culture for 48–96 h at 27°C and His-tagged protein-purified using Ni2+-NTA agarose (Invitrogen) according to standard procedures ...
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bioRxiv - Molecular Biology 2020Quote: Plasmid encoding the N-terminally His-tagged Msm HelD protein was prepared by the GeneArt® Plasmid Construction Service (Thermofisher). Gene construct for HelD expression was designed by codon-optimized back translation of gene MSMEG_2174 from Msm (strain ATCC 700084 / mc2 155 ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged protein was purified from the cleared cell lysate by nickel affinity chromatography (Ni-NTA agarose beads, Invitrogen). After washing with increasing concentrations of imidazole ...
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were cultured on 6-well plates coated with Vitronectin Recombinant Human Protein (Invitrogen) at a concentration of 0.5 µg/cm2 ...
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bioRxiv - Biochemistry 2021Quote: ... the N-terminal His-tagged protein was purified in a single step protocol using HisPur Ni-NTA resin (Thermo Fisher Scientific), equilibrated at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The 6X His tagged extracellular domain of hPD-L1 WT or 2HA proteins were expressed in the ExpiCHO cell system (ThermoFisher Scientific) and purified by the Ni-NTA agarose (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y cells were transfected with either an empty vector or with a vector expressing His-tagged human p35 (gift from Dr. Harish Pant) using Lipofectamine 3000 (Life Technologies, Carlsbad, CA). Two days after the transfection ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human TGF-β1 (Life Technologies) was prepared in sterile water with 0.1% BSA following the manufacturer’s instructions and diluted in 50:50 media.
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bioRxiv - Cell Biology 2022Quote: ... were combined with 0.36 μg of recombinant human protein CDK1/Cyclin B (Thermo Fisher, PV3292), 1x Kinase Buffer (Cell Signaling ...
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bioRxiv - Bioengineering 2022Quote: ... the medium was supplemented with Human Interferon-gamma Recombinant Protein (IFN-γ) (ThermoFisher, Cat. #. PHC4031) at a concentration of 50 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: Reconstitute Human FGF-basic (FGF-2/bFGF) Recombinant Protein (here we use ThermoFisher 13256-029). Briefly ...
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bioRxiv - Biochemistry 2020Quote: N-terminally His-tagged P-Rex2 was synthesised and cloned into pFastBac1 (Invitrogen). His-tagged PTEN ...
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bioRxiv - Microbiology 2021Quote: ... and His-tagged mCherry (strain He/80/FR) were synthesized by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: His-tagged relaxins were expressed as secreted proteins in Expi293F cells containing a stably integrated tetracycline repressor (Expi293F tetR, Thermo Fisher Scientific) grown in Expi293 media (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Recombinant His-HMGB1 was produced in 293-freestyle cells (ThermoFisher Scientific). Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of 6xHis-tagged proteins and Strep-tagged proteins was tested with 6xHIS TagAntibody HIS.H8 (Invitrogen Co.) and StrepMAB-Classic ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the His-tagged proteins on the cell surface were then visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA) using FUSION Solo S documentation system (Vilber ...
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bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human GDNF (Thermo Fisher), 10ng/mL recombinant human CNTF (Thermo Fisher)).
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bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human BDNF (Thermo Fisher), 10ng/mL recombinant human GDNF (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human CNTF (Thermo Fisher)).
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bioRxiv - Cell Biology 2019Quote: ... coli-derived recombinant human insulin (Gibco, A11382IJ), 64 μg ml−1 L -ascorbic acid 2-phosphate trisodium salt (Wako ...
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bioRxiv - Bioengineering 2021Quote: ... on recombinant human vitronectin (50μg/ml; Gibco) coated plates ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 ng/ml human recombinant EGF (Invitrogen), 10 ng/ml bFGF (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... purified recombinant human IL-8 (ThermoFisher Scientific) and CXCL1 (Sigma-Aldrich) ...
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Differential expression of transposable elements in stem cell lineages of the preimplantation embryobioRxiv - Developmental Biology 2020Quote: ... 25 ng/mL human recombinant FGF4 (Gibco), and 1 μg/mL heparin (Sigma-Aldrich)] as previously described (Tanaka et al. ...
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bioRxiv - Cell Biology 2021Quote: ... 0.15 ng of human recombinant VEGF (Gibco) were co-injected with 1.0×106 cells of WS-iMSC.
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bioRxiv - Genetics 2019Quote: ... 10 ng/ml recombinant human FGF (Gibco), 10 ng/ml recombinant human EGF (Peprotech ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 ng/mL Human Recombinant EGF (Gibco), and 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 ng/mL recombinant human GDNF (Gibco), and 5 μM TRO19622 (Tocris) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.5 ng/mL human recombinant bFGF (Gibco), 1% Penicillin/Streptomycin (Wisent Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... 0.01 mg/mL human recombinant insulin (Gibco), and 5 µg/mL plasmocin (Invivogen ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ng/mL recombinant human bFGF (Gibco) and 10 ng/mL recombinant human EGF (Peprotech) ...
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bioRxiv - Cell Biology 2023Quote: ... coli-derived recombinant human insulin (Gibco, A11382IJ). Contracting cells were noted from day 8 ...
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bioRxiv - Neuroscience 2023Quote: Human recombinant TNF-α and FGF2 (Gibco) were dissolved in sterile water per manufacturer’s instructions and stored at -20 °C ...
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bioRxiv - Genomics 2023Quote: ... 10 ng/mL human recombinant EGF (ThermoFisher), 5.5mM D-glucose (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 ng/mL recombinant human EGF (Gibco), 100 ng/mL recombinant human noggin ...