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Citations for Thermo Fisher :
451 - 500 of 7303 citations for Recombinant Human S100A3 His & MBP tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Microbiology 2022Quote: ... The internalization of the ACE2-HA protein and anti-hACE2 mAbs was then evaluated by staining with goat anti-mouse Alexa Fluor™ 594 (to detect the HA-tagged hACE2) and/or goat anti-human Alexa Fluor™ 488 antibodies (to detect the hACE2 mAbs) (ThermoFisher Scientific). Images were captured using a DeltaVision OMX SR imaging system (GE Healthcare).
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bioRxiv - Physiology 2019Quote: ... AlexaFluor-tagged secondary antibodies (Life Technologies) were used to detect primary antibodies ...
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bioRxiv - Systems Biology 2020Quote: ... 4μM fluorescein-tagged peptide (#F1314, Invitrogen) was loaded in the left side of the set-up ...
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bioRxiv - Cell Biology 2020Quote: ... fluorochrome-tagged secondary antibodies (Life Technologies) were added to coverslips for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... and fluorescently tagged phalloidin (Invitrogen #A34055) were included during the secondary antibody incubation step ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended in B8 media with varying concentrations if iMatrix laminin and/or truncated recombinant human vitronectin (ThermoFisher #A14700). Cells were seeded at 5,000 cells/cm2 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 level ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Cell Biology 2023Quote: ... custom micropatterned glass coverslips presenting 500 µm groWTh areas (CYTOOCHIPS Arena 500A) were covered with 800µL of 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in PBS+/+(Gibco) for 3h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... hESCs were grown in glass surfaces previously coated with 10μg/ml recombinant human laminin 521 (BioLamina) diluted in PBS+/+(Gibco) for 3h at 37°C ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and seeded onto new 6-well plates at 2,500 cells/cm2 with 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700). After allowing cells to adhere overnight ...
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bioRxiv - Genomics 2023Quote: ... and then at time 0 media was exchanged for complete HaCat media + 20ng/mL human recombinant EGF (Gibco PHG0311L) for all wells except the 0hr timepoint ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (ThermoFisher Scientific, Waltham, MA, USA) (S12+ media ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in keratinocyte serum-free medium supplemented with 0.05 mg/ml bovine pituitary extract and 5 ng/ml human recombinant epidermal growth factor (Gibco). T24 human bladder cancer cells were cultured in HAM’s F12 medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin/glutamine (PSG) ...
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bioRxiv - Biochemistry 2024Quote: ... The human Dicer/TRBP enzyme complex was pre-mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
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bioRxiv - Cell Biology 2024Quote: IMR90 fibroblasts were differentiated to myofibroblasts with 5ng/mL Recombinant Human Transforming Growth Factor -β1 (TGF-β1) (Invitrogen, #PHG9204) for 48h in serum free media ...
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% HI FBS (Gibco A31604), and pen/strep and seeded into 96-well plates 24 hr prior to treatment ...
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bioRxiv - Neuroscience 2022Quote: ... and pcDNA3.1/myc-His (Invitrogen). Constructs for hTara mutants were prepared by cloning into pEGFP-C3 ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-FBS (Gibco, 16140071) + 10% HI-Horse Serum (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... His-bind magnetic Dynabeads (Invitrogen) were washed with TBS containing 0.05% Tween-20 (TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco).
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco). Neutrophils were recovered from the bottom of the tube.
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bioRxiv - Microbiology 2023Quote: ... GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E) ...
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bioRxiv - Cancer Biology 2023Quote: ... with 2% HI FBS (Gibco) and 0.4% 0.5M EDTA (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-His (ThermoFisher scientific, rd230540a), anti-SETD6 (Genetex ...
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bioRxiv - Microbiology 2024Quote: ... and 10% HI FBS (Gibco). Media were supplemented with interleukin-2 (IL-2 ...
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bioRxiv - Cell Biology 2019Quote: mouse anti-MBP (1:5000, R29, Cat. #MA5-14122, Thermo Fisher Scientific), mouse anti-BRCA2 (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... MBP-Klf4 was buffer exchanged using Zeba Spin Desalting Columns (Thermo Scientific) into Klf4 buffer (25 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... MBP-PatMC was labelled with 4-fold molar excess NHS-Fluorescein (Thermofisher) for 1 hour at room temperature in 150 mM NaCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... and re-suspended at a density of 104 cells/mL in pre-warmed complete minimum essential media (MEM) that contains 5 mg/mL human recombinant insulin (Gibco), 1X MEM non-essential amino acids (NEAA ...
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bioRxiv - Genomics 2020Quote: ... Both cell lines were maintained in culture vessels coated with a matrix of Vitronectin human recombinant protein (ThermoFisher Scientific, A14700) and batch fed daily with mTeSR (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... HFKs and iHFKs were grown and maintained in keratinocyte-serum-free media (KSFM) supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Invitrogen). IMR-90s normal human diploid lung fibroblasts were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... recombinant human epidermal growth factor (5 ng/ml) and additionally supplemented with freshly added recombinant human fibroblast growth factor-basic (1 ng/ml; Gibco). hCMEC/D3 cells used for the experiments were between passages 6 and 11 ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
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bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... and recombinant human Interleukin-2 (rhIL-2, 100 units/mL)) in the presence of anti-CD3/CD28 Dynabeads (Fisher Scientific) at a bead:cell ratio of 1:1 ...