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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Recombinant Human ROR1 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Neuroscience 2023Quote: ... The purified His-TEV-FAM19A5 proteins were digested overnight at 30°C with AcTEV protease (Invitrogen) to remove the His-tag and TEV sequence ...
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bioRxiv - Developmental Biology 2019Quote: ... CD117-APC (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... CD4 APC (Invitrogen), CD4 APCCy7 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... SlamF6-APC (Invitrogen), CD127-Superbright 436 (eBioscience) ...
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bioRxiv - Microbiology 2020Quote: ... labeled and hybridized to separate arrays using the GeneChip Human Exon 1.0 ST Array (Affymetrix, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Each gel included a migration standard comprised of Di-I labeled human LDL (L3482, ThermoFisher Scientific) that was diluted in cryoprotectant and stored in frozen aliquots (see recipes) ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: DNA coding for full length HA-tagged human NEGR1 (NM_173808.2) was synthesized using the GeneArt® Gene Synthesis service (Life Technologies) and subcloned into the pcDNA3 vector ...
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bioRxiv - Biochemistry 2022Quote: Chimeric SHh precursor encoding HA-tagged NLuc fused to native and mutant forms of human Sonic HhC autoprocessing domain were assembled in pDisplay (Invitrogen). Fragments encoding Sonic HhC were inserted into pDisplay using PstI and XhoI ...
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Peptide Arrays of Three Collections of Human Sera from Patients Infected with Mosquito-Borne VirusesbioRxiv - Microbiology 2019Quote: ... The arrays were then incubated for 1 h with goat anti-human IgG tagged with Alexa Fluor® 488 (Invitrogen) as secondary antibody ...
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit IgG antibodies (1:500 for IF) and recombinant human BMP2 (#PHC7145) were from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Biophysics 2019Quote: ... FLAG-tagged hZP3 and HA-tagged hZP4 were synthesized (GenScript; GeneArt/Thermo Fisher Scientific) and subcloned into pHLsec329 ...
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bioRxiv - Microbiology 2021Quote: ... CD49b (clone DX5, APC-Cy7, Biolegend or APC-e780, Affymetrix Inc) and MHC-II (clone M5/114.15.2 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed in PBSE-d and labeled with a goat anti-rabbit APC conjugated secondary antibody (1:500 dilution, ThermoFisher Scientific, cat # 31984) and DAPI (1:5000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) and B-cell (CD19-eFluor450, ThermoFisher Scientific, Grand Island, NY) markers after blocking with anti-mouse CD16/32 antibodies (BioLegend) ...
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bioRxiv - Biochemistry 2019Quote: ... anti V5-HRP conjugate (to detect mSCARLET and other V5 tagged fluorescent proteins, catalog number 46-0708, Invitrogen), rabbit polyclonal anti TAL1 IgG (catalog number A305-300A ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μg of GST tagged protein was conjugated to Pierce™ Glutathione Magnetic Agarose Beads (Thermo Fisher, 78602), for 1hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM maltose.) SKD3-MBP fusion protein was cleaved with a 6His-tagged AcTEV™ protease (Thermo Fisher) at 4 °C for 96 h in the amylose elution buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-tagged bait proteins were immobilized in a 96-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark) at 4°C for 18 hours (10 μg per protein in 100 μl PBS) ...
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bioRxiv - Biophysics 2021Quote: ... we labeled 5-30 μM protein with 2 mM DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... we labeled 10 μM protein with various concentrations of DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Fluorescently labeled proteins were detected using an iBright FL1000 imaging system (Invitrogen, CA, USA). The Western blot results were quantified using ImageJ software (National Institutes of Health ...
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bioRxiv - Cell Biology 2022Quote: ... was fluorescently labeled with Alexa Fluor™ 488 Microscale Protein Labeling Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was digested and TMT labeled using the TMTduplex isobaric mass tagging kit (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... or Alexa-494 gelatine (labeled with molecular probes protein labeling kit, Thermo Fisher Scientific) to make the spot boundaries visible ...
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bioRxiv - Biochemistry 2020Quote: ... Labeled Protein A (200 µg) was then conjugated to Dynabeads M-280 Tosylactivated (Invitrogen) following the provided protocol to produce 10 mg of beads at a final concentration of 20 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... These sonicated microsomes (protein concentration 1.5 mg/ml) were labeled with BODIPY-C12 (Invitrogen™ ...
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bioRxiv - Cell Biology 2020Quote: ... was previously labeled using an Alexa Fluor 633 Protein Labeling Kit (A20170, Molecular probes) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested using LysC/Trypsin and labeled for multiplexing using TMT labelling (ThermoFisher). Phospho proteins were collected using pSTY enrichment and fractionation and flow through from the pSTY enrichment was used for total protein-level analysis ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were labeled with EZ-Link™ NHS-PEG4-Biotin (Thermo Scientific™ A39259) in a 1:1 ration at 37 °C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... eOD-GT8KO 60mer nanoparticles were labeled with Pacific Blue protein labeling kit (Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... To isolate labeled proteins supernatants were incubated with NeutrAvidin™ agarose (Themo Fisher Scientific) for 60 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... or IgG1 Fc (human) protein (Thermo Fisher Scientific, Rockford, IL) was added to a final concentration of 10 mg/mL and samples were incubated with gentle mixing for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... cleared lysates were mixed with Protein G/A (50:50) recombinant protein G agarose beads (catalog #15920-010, Invitrogen, Carlsbad, CA) coupled with anti-HA (catalog #3724S ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
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bioRxiv - Cell Biology 2024Quote: SK-Mel28 cells were grown on coverslips and transiently transfected with ribonucleprotein complexes consisting of purified recombinant Cas9 protein (Truecut Cas9 Protein v2, Invitrogen, #36499) and synthetic guide RNAs (Invitrogen TrueGuide Synthetic sgRNA #35514 or Negative Control ...
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bioRxiv - Neuroscience 2020Quote: ... Labeled proteins were recovered from equivalent amounts of protein by batch affinity chromatography with streptavidin-coupled M280 Dynabeads (Thermo Fisher), 4°C ...
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bioRxiv - Molecular Biology 2023Quote: Bacterially expressed and purified NEMO proteins were labeled with the Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher, A30006) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.