Labshake search
Citations for Thermo Fisher :
651 - 700 of 6401 citations for Recombinant Human PLAT None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... Recombinant EcoSSB and Sequenase© were purchased from Thermofisher. T7DNAp was purchased from NEB ...
-
bioRxiv - Microbiology 2023Quote: ... recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), and then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
-
bioRxiv - Microbiology 2023Quote: ... Recombinant protein A/G conjugated to HRP (Thermo Fisher) diluted 1:500 in TBST was added each well and the plates were incubated for 1 hour at 37°C then washed four times with TBST ...
-
bioRxiv - Cell Biology 2022Quote: ... 60 units of RNaseOUT Recombinant RNase Inhibitor (Invitrogen, 10777019) was added to each sample and vortexed for 5 seconds ...
-
bioRxiv - Microbiology 2023Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or HCoV-OC43 N-His (Sino Biological # 40643-V07E ...
-
bioRxiv - Immunology 2023Quote: ... recombinant SARS-CoV-2 S protein (RP-87680, Invitrogen) or recombinant SARS-CoV-2 spike RBD protein (40592-V08H ...
-
bioRxiv - Cell Biology 2023Quote: ... 100U/mL RNAse OUT Recombinant Ribonuclear Inhibitor (ThermoFisher 10777019), and 1X complete EDTA-free Protease Inhibitor Cocktail (Sigma 11836170001)] ...
-
bioRxiv - Cancer Biology 2023Quote: ... 50 μl of Recombinant Protein G – Sepharose beads (Invitrogen) per condition were washed 3 times in IP buffer ...
-
bioRxiv - Immunology 2023Quote: ... Recombinant mouse (rm)IFN-γ was purchased from ThermoFisher Scientific.
-
bioRxiv - Neuroscience 2023Quote: ... with or without recombinant IL-1β (50ng/ml)(Invitrogen; Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... 50 ng/ml recombinant EGF (PMG8041, Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
-
bioRxiv - Immunology 2024Quote: Recombinant proteins were expressed in Expi293F cells (Life technologies). Full-length spike proteins were cloned into the pCAGGS mammalian expression vector as previously described79,80 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
-
bioRxiv - Molecular Biology 2021Quote: ... We immunoprecipitated GFP-tagged Znf684 with 1 μg anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
-
bioRxiv - Cancer Biology 2019Quote: ... PCR8-KLK4-KLKP1 (DDK tagged) was recombined with pLenti6/V5-Dest™ (Life Technologies) or pAD/CMV/V5-Dest™ (Life Technologies) ...
-
bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-mouse IgG (H+L) antibody (Invitrogen), before being acquired with BD FACSCanto II (BD Biosciences) ...
-
bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
-
bioRxiv - Neuroscience 2022Quote: ... and/or AlexaFluor-594-tagged goat anti-guinea pig IgG (1:500; Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
-
bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
-
bioRxiv - Bioengineering 2020Quote: ... sections were incubated with the appropriate Alexa 546-tagged secondary antibody (Invitrogen, Carlsbad, CA) for 2 hrs at room temperature (RT) ...
-
bioRxiv - Synthetic Biology 2022Quote: All antigens were His-tagged and purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer [20 mM imidazole ...
-
bioRxiv - Microbiology 2020Quote: ... SV5-tagged proteins were detected using mouse anti-SV5 mAb (1:5000, Life Technologies); HA-tagged proteins were detected by anti-HA mAb (clone HA-7 ...
-
bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged RNF219 and CNOT1 cDNAs were cloned into pCDNA5/FRT-TO vector (Invitrogen). The expression vectors were then transfected into HEK-293 Flp-In-TRex cells and followed by selection with hygromycin to generate stable cell lines ...
-
bioRxiv - Molecular Biology 2019Quote: ... GST-tagged GSK3β constructs were cloned into the pDEST™27 vector (ThermoFisher Scientific) as described previously66 ...
-
bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
-
bioRxiv - Biochemistry 2021Quote: ... GST-tagged RASSF5 (GST-RASSF5) sequence was transferred into the pDEST15 expression vector (Thermofisher), and then expressed and purified from BL21-A1cells ...
-
bioRxiv - Cancer Biology 2020Quote: ... HA-tagged RON was cloned into the expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) by fusion PCR ...
-
bioRxiv - Immunology 2020Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher). To eliminate nonspecific binding proteins ...
-
bioRxiv - Molecular Biology 2020Quote: ... His-tagged hAgo2 was purified from Sf9 cells using a baculovirus system (Thermo Fisher). Cells were lysed in Lysis Buffer (300mM NaCl ...
-
bioRxiv - Immunology 2022Quote: ... and RAW 264.7 EROS FLAG-tagged cells were maintained in DMEM medium (21969035, ThermoFisher) containing 10 % FBS (F7524 ...
-
bioRxiv - Biochemistry 2022Quote: ... His-tagged protein was labeled with Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) per manufacturer’s recommendations ...
-
bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
-
bioRxiv - Immunology 2022Quote: ... was used to generate Flag-tagged Prom1 clones and deletion mutants in pcDNA3.1+ (Invitrogen). The primers used for cloning are listed in Supplementary Table 1.
-
bioRxiv - Bioengineering 2023Quote: ... A fluorescently tagged secondary antibody (Goat anti-rabbit, Alexa Fluor 647, Thermo Fisher Scientific) diluted in the same antibody solution was applied for 1 h ...
-
bioRxiv - Neuroscience 2023Quote: ... HA-tagged scFvs were detected using mouse mAb 2-2.2.14 (ThermoFisher Catalog# P126183, RRID:AB_10978021). The following day sections were washed 4 x 5 min with vehicle ...
-
bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 488-tagged donkey-α-goat secondary antibody (1:1000; Molecular Probes) for one hour ...
-
bioRxiv - Developmental Biology 2022Quote: ... and tagged Stat3 were synthesized with linearized plasmids using T7 mMessage mMachine kit (Invitrogen). For overexpression essays ...
-
bioRxiv - Cell Biology 2023Quote: ... and His6-tagged VASP (Benz et al., 2009) using Lipofectamine 2000 (Invitrogen, Karlsruhe, Germany). Cells were lysed in buffer A (40 mM HEPES-NaOH ...
-
bioRxiv - Molecular Biology 2023Quote: ... WRN Halo-tagged U2OS cells were seeded in GlutaMAX- supplemented DMEM (Gibco, 10566-016) with 10% FBS and 1% Penicillin/Streptomycin at 6x103 cells per well ...
-
bioRxiv - Developmental Biology 2023Quote: ... The primary labelling was detected using appropriate Alexa-Flour tagged secondary antibody (ThermoFisher Scientific).
-
bioRxiv - Neuroscience 2023Quote: ... N2A cells were transfected with different HA tagged WDR47 variants using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... 6xHis-tagged CapZ was purified as previously described30,49 using HisPur Cobalt resin (Thermo Scientific), and separated from high-molecular weight contaminants by size-exclusion chromatography using a Sephacryl S-200 HR column (Cytiva/GE Healthcare) ...
-
bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
-
bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
-
bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
-
bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
-
bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
-
bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...