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Citations for Thermo Fisher :
351 - 400 of 7306 citations for Recombinant Human Met His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Immunology 2020Quote: ... Recombinant human TNF-α (PHC3011) and antibody against OAS3 (PA5-31090) was from Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
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bioRxiv - Bioengineering 2022Quote: ... the medium was supplemented with Human Interferon-gamma Recombinant Protein (IFN-γ) (ThermoFisher, Cat. #. PHC4031) at a concentration of 50 ng/mL ...
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bioRxiv - Pathology 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
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bioRxiv - Cell Biology 2023Quote: Reconstitute Human FGF-basic (FGF-2/bFGF) Recombinant Protein (here we use ThermoFisher 13256-029). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Thermo Fisher Scientific AG), 1% penicillin and streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant human Epithelial Growth Factor 5 µg.L-1 (Ref AF-100-15; PEPROTECH, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and plated in the fibroblast medium onto truncated recombinant human vitronectin (VTN-N, Gibco, A14700)-coated plates ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pretreated for 1 h with 10 ng/mL human recombinant FGF-2 (Gibco) or vehicle (sterile water) ...
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bioRxiv - Neuroscience 2023Quote: ... Purified recombinant human antibodies (rHumAbs) were desalted (Zeba™ Spin Desalting Columns, Thermo Fisher 89891) and concentrated using a Christ Rotational Vacuum Concentrator System ...
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bioRxiv - Immunology 2024Quote: ... and recombinant human interleukin 4 (rhIL-4) (300 U/mL; Thermo Fisher Scientific, Waltham, MA). On day 2 and day 5 post-monocyte isolation ...
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bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
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bioRxiv - Immunology 2022Quote: ... 1% MEM NEAA and 50 Units/mL recombinant human IL-2 (Myltenyi; #130-097-745) with Dynabeads™ Human T-activator CD3/CD28 (Gibco, #11132D) the same day of the purification (day 0 ...
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bioRxiv - Immunology 2019Quote: ... total PBMC or IEL were cultured in the presence or absence of recombinant human osteopontin (2 μg/ml) (R&D) and anti-human-CD44 (5 μg/ml) (Thermofisher; clone IM7).
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stored using a controlled rate freezer (Cryo Met Freezer, Thermo Fisher) at −1°C/min until the temperature reached −100°C ...
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bioRxiv - Bioengineering 2023Quote: Rhodamine-tagged dextran (Invitrogen) was encapsulated into AlgLung and S-AlgLung hydrogels at a concentration of 0.5 mg/ml ...
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Cancer Biology 2019Quote: ... iPSC line WTC-11 (Coriell Institute) was maintained on recombinant human vitronectin[36,37](Thermo Fisher Scientific), coating six-well tissue culture plastic plates (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
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bioRxiv - Microbiology 2020Quote: Mice engrafted with J-Lat cells were treated with recombinant human TNF-α (Cat. #PHC3011, Gibco), a potent and well-known LRA ...
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bioRxiv - Genomics 2022Quote: ... mTESR1 is exchanged with the addition of 5 ng/ml Recombinant Human BMP4 (Fisher Scientific, 314BP010). Exposure to BMP4 triggers differentiation of cells and is considered 0 hours for experimental purposes ...
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bioRxiv - Cell Biology 2022Quote: ... with <0.9% rabbit brain tissue contaminant) and another tube with human recombinant Thromboplastin (Thermo fisher scientific). Next to remove cells ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 12 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) (Thermo Fisher Scientific, USA) was included in the culture medium ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: iPSCs from all lines were cultured in StemFlexTM medium on human recombinant vitronectin (Thermo Fisher Scientific) as described (34,35,37) ...
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bioRxiv - Physiology 2024Quote: ... or 0.75 UI human recombinant insulin/kg body weight for ITTs (catalog no. 12585014, Gibco™). Tail glucose measurements were then taken at the indicated time points in the figures to measure glucose clearance.
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bioRxiv - Immunology 2024Quote: ... recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) (300U/mL; Thermo Fisher Scientific, Waltham, MA) and recombinant human interleukin 4 (rhIL-4 ...
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bioRxiv - Molecular Biology 2021Quote: GPI-APs were HA-tagged by cloning their coding cDNA amplified from the human cDNA library or from Invitrogen plasmids ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed 1x and incubated with Met-free DMEM (Gibco, Thermo Fisher Scientific) supplemented with 2 mM Gln for 30 min including LRRK2 inhibitors or 0.1% DMSO ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed 1x and incubated with Met-free DMEM (Gibco, Thermo Fisher Scientific) supplemented with 2 mM Gln for 30 min including LRRK2 inhibitors or 0.1% DMSO ...
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bioRxiv - Biochemistry 2022Quote: ... The medium was removed and the cells were starved in Met-deficient medium (Gibco) supplemented with 1 % (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed 1× and incubated with Met-free DMEM (Gibco, Thermo Fisher Scientific) supplemented with 2 mM Gln 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed 1× and incubated with Met-free DMEM (Gibco, Thermo Fisher Scientific) supplemented with 2 mM Gln 30 min ...
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bioRxiv - Microbiology 2023Quote: ... The following pairs of primary antibodies were used: mouse anti-c-Met (#370100, Invitrogen) and rabbit anti-E-cadherin antibodies (#GTX100443 ...
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bioRxiv - Microbiology 2023Quote: ... Specific epithelial cell proteins were detected by immunoblotting with anti-c-Met (#370100, Invitrogen), anti-EGFR (#2234 ...
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bioRxiv - Molecular Biology 2021Quote: ... cerevisiae EKY3/pYES2-TOP1 induced for expression of wild-type human TOP1with the PrepEase Histidine-Tagged Protein Purification Midi Kit – High Specificity (from Affymetrix) according to the manufacturer’s procedures ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...