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Citations for Thermo Fisher :
601 - 650 of 7095 citations for Recombinant Human MME Fc Chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed thrice with ice cold PBS2+ and surface BBS-DAT chimeras were labeled with 120μM biotinylated α-bungarotoxin (α-BTX-b, Thermo Fisher) in PBS2+ ...
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bioRxiv - Plant Biology 2020Quote: ... the pEn_Sa_Chimera entry plasmid was digested by BbsI and annealed oligonucleotides bearing complementary overhangs were ligated through T4 DNA ligase (ThermoFisher Scientific, USA) (S2 Table 1) ...
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bioRxiv - Molecular Biology 2019Quote: Luciferase activity assay in the supernatants of transfected C6/36 cells with chimeras and wild type constructions were determined with Pierce™ Gaussia Luciferase Glow Assay Kit (Thermo Scientific). Percentage of luciferase activity was normalized with DMSO treatment.
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bioRxiv - Biophysics 2022Quote: ... and the cells were transfected with the indicated HaloTag- and/or EGFP-protein chimeras using Lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Cell Biology 2020Quote: ... 25ng/mL recombinant murine EGF (Gibco), 50ng/mL recombinant human HGF (Peprotech) ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... and RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... we added recombinant mouse IFNγ (ThermoFisher), 2-deoxyglucose (Abcam) ...
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bioRxiv - Microbiology 2022Quote: ... and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). Next ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant IGF1 (Life Technologies, cat# PHG0071) and picropodophyllin (PPP ...
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bioRxiv - Genomics 2019Quote: ... 50ng/mL recombinant murine EGF (Gibco), and 100ug/mL recombinant human Noggin (Peprotech) ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant vitronectin (A14701SA, Thermo Fisher) was diluted in DPBS at the concentration of 1:200 to get the final concentration of 2.5 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant TNFα (Thermo Fisher, cat# PHC3015) was reconstituted at 0.1mg/ml in water ...
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bioRxiv - Cell Biology 2023Quote: ... mouse recombinant Egf (Life Technologies #PMG8043), 100 ng/mL mouse recombinant Noggin (Peprotech #250-38 ...
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bioRxiv - Molecular Biology 2019Quote: 2.5D-Fc and 2.5F-Fc fusions were labeled with the succinimidyl ester derivative of Alexa Fluor 488 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... washed with FC buffer and resuspended in FC buffer including viability die and analyzed on Attune (Thermofisher Scientific). The permeabilized cells were excluded from the analysis due to intracellular staining.
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bioRxiv - Bioengineering 2023Quote: ... Bound SiRPα-Fc was detected with a secondary biotinylated rabbit anti-IgG Fc monoclonal antibody (Thermo Fisher Scientific) followed by HRP-conjugated streptavidin (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Atg3 KO MEF stably expressing ATG3 WT or ATG3 chimera were generated as follows: HEK293T cells were transfected using Lipofectamine 2000 reagent (Thermo Fisher Scientific, 11668019) with retroviral plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 30% heat-inactivated FCS (Gibco) and penicillin-streptomycin in each well ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% heat-inactivated FCS (Gibco) and penicillin-streptomycin and 30 ng/mL Doxycycline for 10 days prior to experiments.
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bioRxiv - Immunology 2021Quote: ... Fc block (anti-CD16/CD32, 101302 Affymetrix) was included to minimize nonspecific antibody binding ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented with 10% FCS (Gibco 10270-106), 100 U/ml LIF (homemade) ...
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bioRxiv - Immunology 2021Quote: ... and 10% heat-inactivated (FCS; Thermofisher, Scientifix). Cultures were supplemented with 10IU IL-2 (BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... FCS and antibiotics were purchased from Invitrogen/Thermofisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10 % vol/vol FCS (Gibco). Splenocytes were mashed through a 100 μm cell strainer (Falcon) ...
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bioRxiv - Cell Biology 2021Quote: ... 10% Fetal Calf Serum (FCS, Life Technologies), and 100 μg/mL Primocin (Invivogen ...
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bioRxiv - Cancer Biology 2020Quote: ... 10% Fetal Calf Serum (FCS, Thermo Scientific), 244 μM penicillin and 172 μM streptomycin ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% heat-inactivated FCS (Gibco). YFV strain French Neurotropic (National Collection of Pathogenic Viruses ...
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bioRxiv - Systems Biology 2020Quote: ... containing 5% dextran-charcoal treated FCS (Invitrogen) and antibiotics (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% heat-inactivated FCS (Invitrogen) and 0.05 mM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: ... with 10 % fetal calf serum (FCS, Gibco) and Antibiotic-Antimycotic® (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... 20% fetal calf serum (FCS) (Life Technologies), 2% UltroserG (Pall Life Sciences) ...
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bioRxiv - Cell Biology 2020Quote: ... and 10% fetal calf serum (FCS; Gibco). Cell cultures were maintained in 5 % CO2 in air at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% FCS (Life Technologies, #10500064), 50 units/ml penicillin and 50 µg/ml streptomycin (Lonza ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% (v/v) FCS (GIBCO) and antibiotics (100 U/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... 10% Fetal calf serum (FCS) (Invitrogen, Ireland), and 1% penicillin/streptomycin (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... 10% fetal calf serum (FCS, Life Technologies), 2% B-27 and 0.5 mM L-glutamine (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 10% FCS and GlutaMAX (Invitrogen). Cells were then pelleted at 140 × g for 5 min ...
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bioRxiv - Microbiology 2019Quote: ... 10 % v/v FCS (Gibco, tet free), Pen/Strep (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... heat-inactivated FCS (Fetal Calf Serum, Invitrogen), and 1-Oleoyl lysophosphatidic acid (LPA ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% Fetal calf Serum (FCS, Fisher Scientific) and Gentamycin (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal calf serum (FCS, GIBCO), 100U/ml Pen-Strep (GIBCO ...
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bioRxiv - Genetics 2020Quote: ... 10% fetal calf serum (FCS, Thermo Scientific) 100 U ml−1 penicillin ...