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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Recombinant Human Lysyl Oxidase Like 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... while islets were seeded onto the same coverslips coated with recombinant human laminin-521 (Gibco, #A29248, 0.5 μg/cm 2). Cells/islets were allowed to attach overnight ...
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bioRxiv - Microbiology 2020Quote: ... All human MARCH cDNAs were cloned into pCDNA3.1/myc-His A (Invitrogen) and were then subcloned into pBJ5 ...
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bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 U RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen, 10777019), 10 U SuperscriptII (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... 2 U RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen, 10777019), 10 U SuperscriptII (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: N-terminally 6xHis-tagged human Gβ1 (S2-N340) and human Gγ2 (M1-C68) were synthesized and separately cloned into pFastBac1 (Thermo Fisher) and expressed as described above ...
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bioRxiv - Physiology 2020Quote: ... MCF-10 human mammary epithelial cells and COS-7 fibroblast-like monkey cells were maintained in DMEM (Fisher Scientific) supplemented with 10% fetal bovine serum and 2% penicillin/streptomycin.
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bioRxiv - Microbiology 2021Quote: ... A549 (male, human lung epithelial-like) cells were obtained from ATCC and grown at 37 °C in DMEM (Gibco) supplemented with 10 % FBS (GE Healthcare Life Science ...
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bioRxiv - Biophysics 2021Quote: Detergent extracted His-MBP-tagged proteins were applied to a 40 mL POROS MC 20 Ni2+ column (Applied Biosystems) equilibrated with buffer A (200 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... SmBChE1 and SmAChE3 (fSmChEs) were EcoRI/XbaI cloned into the C-terminal 6-His-tagged pPICZαA expression vector (Invitrogen) to facilitate secretory expression ...
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bioRxiv - Biophysics 2023Quote: ... and the 3C protease was removed using Dynabeads magnetic beads designed for His-tagged protein isolation (Thermo Fisher, 10103D). The supernatant from the magnetic bead pulldown was then concentrated using Amicon 10 kDa centrifugal filter units (MilliporeSigma) ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged TpPyShell together with TEV protease was dialyzed overnight at 4°C in SnakeSkin dialysis tube (Thermo Scientific) against dialysis buffer (50 mM Tris-HCl pH8.0 ...
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bioRxiv - Microbiology 2020Quote: ... with 0.1 ng/mL human recombinant epidermal growth factor (EGF; Gibco) and 0.05 mg/ml bovine pituitary extract (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... and human recombinant insulin (2.5 µg/mL, Thermo Fisher Scientific, 12585014). From Day 1 to 11 ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length recombinant human LRRK2[G2019S] was purchased from Invitrogen (#A15202). GZD-824 was purchased from Cayman Chemical (#21508 ...
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bioRxiv - Pathology 2019Quote: ... CSF-1 Recombinant Human Protein (25ng/mL, Thermo Fisher Scientific PHC9504), and Recombinant Human TGF-β1 (50ng/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 ng/mL of human recombinant epidermal growth factor (Invitrogen PHG0313), 10 ng/mL of basic fibroblast growth factor (Invitrogen PHG0263 ...
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bioRxiv - Microbiology 2020Quote: Recombinant human mAbs were expressed in Expi293 HEK cells (Life Technologies), which were maintained in suspension at 37°C and 8% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... and 1% human recombinant insulin and zinc solution (Gibco, 12585-014). On day 3 ...
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bioRxiv - Cell Biology 2022Quote: Recombinant human (h) PF4 was expressed in Drosophila Expression System (Invitrogen) S2 cells and purified as described22 ...
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bioRxiv - Cancer Biology 2019Quote: ... 50 ng/mL recombinant human EGF (Thermo Fisher Scientific, Waltham, MA), 0.1 mM N-acetyl-L-cysteine (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 2.5 μg / 500 mL EGF recombinant human protein (Gibco) and 1% penicillin and streptomycin (Wisent ...
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bioRxiv - Neuroscience 2021Quote: ... and 20 ng/mL thermostable recombinant human Fibroblast Growth Factor (Gibco)] unless otherwise noted ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.25 and 1 ng/ul human recombinant FGF8 (Thermofisher scientific, # PHG0184) diluted in E2 media by immersion starting at 18hpf ...
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bioRxiv - Bioengineering 2021Quote: ... human recombinant insulin (10 μg ml-1, Life Technologies, 12585-014), IGF-2 (30 ng ml-1 ...
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bioRxiv - Genetics 2022Quote: ... Recombinant human macrophage colony-stimulating factor (CSF1, Gibco-Thermo Fisher, PHC9501) was then added at a final concentration of 100 ng/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 ng/ml recombinant human epidermal growth factor (Invitrogen, Waltham, MA), and 5 μg/ml of follicle-stimulating hormone (Bioniche Animal Health ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/ml human recombinant epidermal growth factor (Fisher Scientific PHG0311), 5 mM glucose (Fisher Scientific A2494001) ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 ng/mL human EGF recombinant protein (Life Technologies, Cat: PHG0311), and 100 IU/mL P/S ...
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bioRxiv - Genetics 2022Quote: ... Recombinant human macrophage colony-stimulating factor (CSF1, Gibco-Thermo Fisher, PHC9501) was then added at a final concentration of 100 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/uL human IL-6 recombinant protein (ThermoFisher PHC0066).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... VEGF ligand (recombinant human protein) was ordered from ThermoFisher (CAT: #PHC9391).
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bioRxiv - Microbiology 2024Quote: ... Treatment with recombinant 10 U/mL human IFNγ (ThermoFisher Scientific RIFNG100) was done at 24 hours post-infection ...
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bioRxiv - Bioengineering 2024Quote: ... 5250 ng/mL human recombinant IL-6 protein (Invitrogen; Waltham, MA) was added to the experimental group and an equal volume of 1X PBS was added to the control group ...
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bioRxiv - Immunology 2021Quote: Purified CD8+ cells were cultured in AIMV medium supplemented with 10% FBS and 100 U/ml recombinant human IL-2 (ThermoFisher Scientific) and used for sorting of CD8+ T cell subsets and for further experiments three days after isolation.
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and 20 U/ml recombinant interleukin-2 (IL-2; Thermo Fisher Scientific) at 37° C ...
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bioRxiv - Neuroscience 2020Quote: ... cells resuspended in 500µl 2% HI-FBS + PBS (ThermoFisher 12484028), and passed through a 40µm nylon mesh cell strainer (FisherScientific 352235) ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant Rep1ΔAD-His and its variants were incubated with 50 μl D–Galactose agarose beads (Thermo Scientific) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: Human iPSC-derived Brain Microvascular Endothelial-like cells were treated with 25 μg/mL fluorescent transferrin conjugate (Thermo Fisher Scientific) and incubated at 37oC for 30 min ...
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bioRxiv - Microbiology 2022Quote: Murine macrophage-like RAW 264.7 cells and human A549 lung epithelial carcinoma cells were cultivated in RPMI 1640 medium (Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Bioengineering 2022Quote: ... supernatants were harvested and filtered with a 0.22 µm membrane.The His-tagged proteins were purified with the HisPur Ni- NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Biochemistry 2019Quote: ... mCherry-(backbone pLV-CMV-bc) and FLAG-His-tagged(backbone Pcdna3) p16INK4A constructs were created using gateway technology (Life Technologies). For cell cycle profiling ...