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Citations for Thermo Fisher :
401 - 450 of 7391 citations for Recombinant Human Histidine rich Glycoprotein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 12 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) (Thermo Fisher Scientific, USA) was included in the culture medium ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: iPSCs from all lines were cultured in StemFlexTM medium on human recombinant vitronectin (Thermo Fisher Scientific) as described (34,35,37) ...
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bioRxiv - Physiology 2024Quote: ... or 0.75 UI human recombinant insulin/kg body weight for ITTs (catalog no. 12585014, Gibco™). Tail glucose measurements were then taken at the indicated time points in the figures to measure glucose clearance.
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bioRxiv - Immunology 2024Quote: ... recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) (300U/mL; Thermo Fisher Scientific, Waltham, MA) and recombinant human interleukin 4 (rhIL-4 ...
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bioRxiv - Molecular Biology 2021Quote: GPI-APs were HA-tagged by cloning their coding cDNA amplified from the human cDNA library or from Invitrogen plasmids ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Biochemistry 2021Quote: ... and a C-terminal hexa-histidine tag (GeneArt Gene Synthesis, Thermo Fisher Scientific). The HexaPro expression plasmid was a gift from Jason McLellan (Addgene plasmid #154754 ...
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bioRxiv - Microbiology 2019Quote: ... ZagA was purified using Ni-NTA beads (Prepease Histidine purification beads, Life Technologies) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... we used a rich variant of M9-glycerol media containing M9 salt (Fisher Scientific), 4 % ultrapure glycerol (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... or 50 μg/mL neutralized rat tail collagen (rich in Coll I) (Gibco, A1048301) for 2 hours to overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... smegmatis cells were cultured in 7H9-rich medium supplemented with Middlebrook ADC (ThermoFisher, B12352). 100 μl frozen stocks were added to 10 ml of 7H9-rich-ADC medium and subcultured once before use ...
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bioRxiv - Biochemistry 2023Quote: AlbuMAX™ II Lipid-Rich BSA and RPMI-1640 were purchased from Thermo Fisher Scientific® (Leicestershire ...
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bioRxiv - Microbiology 2023Quote: ... “seed culture” was grown in Miller LB rich medium (Fisher Scientific, Cat. No. BP1426) from a single colony on an LB agarose plate ...
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bioRxiv - Developmental Biology 2023Quote: ... with 4 mg/mL AlbuMAX™ II Lipid-Rich Bovine Serum Albumin (GIBCO™), 1× MACS NeuroBrew-21 with Vitamin A (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: DNA coding for full length HA-tagged human NEGR1 (NM_173808.2) was synthesized using the GeneArt® Gene Synthesis service (Life Technologies) and subcloned into the pcDNA3 vector ...
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bioRxiv - Biochemistry 2022Quote: Chimeric SHh precursor encoding HA-tagged NLuc fused to native and mutant forms of human Sonic HhC autoprocessing domain were assembled in pDisplay (Invitrogen). Fragments encoding Sonic HhC were inserted into pDisplay using PstI and XhoI ...
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Peptide Arrays of Three Collections of Human Sera from Patients Infected with Mosquito-Borne VirusesbioRxiv - Microbiology 2019Quote: ... The arrays were then incubated for 1 h with goat anti-human IgG tagged with Alexa Fluor® 488 (Invitrogen) as secondary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... HeLa cells were transfected with green fluorescent protein (GFP)-tagged human NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit IgG antibodies (1:500 for IF) and recombinant human BMP2 (#PHC7145) were from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Immunology 2020Quote: ... 0.25% L-Histidine in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). Due to inherent instability ...
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bioRxiv - Immunology 2022Quote: ... 0.25% L-Histidine in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: ... then probing with α-6x-histidine antibody (Rabbit polyclonal, Thermo Scientific Fisher: PA1-983B)at 1:1000 in TBST overnight at 4°C shaking ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... L-histidine and fluorescein isothiocyanate isomer I (FITC) were from Acros Organics (Geel, Belgium). D- mannitol ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Biophysics 2019Quote: ... FLAG-tagged hZP3 and HA-tagged hZP4 were synthesized (GenScript; GeneArt/Thermo Fisher Scientific) and subcloned into pHLsec329 ...
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bioRxiv - Immunology 2021Quote: ... The primary antibodies used were as follows: P-glycoprotein (1:1000, Invitrogen MA1-2652), Caspase-1 (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... VSV pseudotyped with EBOV glycoprotein was grown by infecting Vero cells (Ambion, Austin, TX) and then harvesting via filtration of the supernatant through 0.4 µM filters 24-30 hours after infection ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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ST6GAL1-mediated heterogeneity in sialic acid levels potentiates invasion of breast cancer epitheliabioRxiv - Cancer Biology 2020Quote: ... or 50 μg/mL neutralized rat tail collagen (rich in type 1 collagen) (Gibco, A1048301) for 2 hours at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Nutrient and iron-rich Brain heart infusion (BHI) media (Oxoid, Thermo Scientific, Carlsbad, CA, USA) were also used in liquid cultures and agar plates for cultivating experimental strains.
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bioRxiv - Cell Biology 2022Quote: ... This cell-rich solution was plated on coverslips coated with rat tail collagen I (Gibco) and incubated for 1 hr at room temperature to allow keratocytes to adhere ...
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bioRxiv - Microbiology 2022Quote: ... Microbes were incubated overnight in rich liquid medium (2% proteose peptone (Thermo Fisher Cat#LP0085), 0.5% NaCl (Mercury Cat#1064041 ...