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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL of His-Tag magnetic beads (Dynabeads™ Invitrogen #10103D) was added to each tube ...
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bioRxiv - Microbiology 2023Quote: ... bags were put into the transportation box (AnaeroPack™ 7.0 L Rectangular Jar, Thermo Scientific). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... Aliquots were gradually frozen using a commercial freezing box (Mr. Frosty, Nalgene, Thermo Fisher Scientific) at −80 °C for 24 h before being transferred to liquid nitrogen for long-term storage.
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bioRxiv - Evolutionary Biology 2024Quote: ... Electrophoresis was performed in an Owl™ D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 45 min at 130 V delivered from an Owl™ EC 300 XL power supply (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1β high sensitivity kit from Invitrogen Inc ...
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bioRxiv - Neuroscience 2020Quote: ... human fibroblasts were cultured in fibroblast culture medium [DMEM high glucose (Life Technologies) plus 10% FBS (Life Technologies)] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1β high sensitivity kit from Invitrogen. The sample of ELISA used in the present study consist of cell culture media (referred to as sample hereafter ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The ELISA was performed using Human IL-1b high sensitivity kit from Invitrogen. The samples for ELISA were media collected after treating the cells with drugs at 100uM ...
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bioRxiv - Cell Biology 2024Quote: Human Embryonic Kidney (HEK) 293T cells were cultured in DMEM high glucose (Invitrogen), supplemented with 10% fetal bovine serum (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... H10,14,243F GPR65 or B2AR N-terminally tagged with FLAG were cultured in DMEM high glucose (Cytiva, SH3024301) supplemented with 10% fetal bovine serum (FBS; Gibco, 26140079). Stable cell lines expressing one of the constructs were generated using Geneticin (Gibco ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Biophysics 2019Quote: ... FLAG-tagged hZP3 and HA-tagged hZP4 were synthesized (GenScript; GeneArt/Thermo Fisher Scientific) and subcloned into pHLsec329 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant viruses were generated in Vero E6 cells (ATCC-CRL1586) grown in DMEM high glucose media (Gibco #11995065) supplemented with 10% Hyclone Fetal Clone II (GE #SH3006603HI) ...
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... MEF cells were grown in high-glucose Dulbecco’s Modified Eagle Medium (DMEM; Cytiva) supplemented with 10% Heat-Inactivated Fetal Bovine Serum (HI FBS; Life Technologies), 10 mL per L of Antibiotic Antimycotic Solution (Penicillin/Streptomycin/Amphoterichin B ...
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bioRxiv - Cell Biology 2022Quote: ... established mature DB-VOs at day 15 from diabetic iPSCs were started to culture in high glucose concentration (33mM, adapted from our previous publication (Yang et al., 2020)) plus 1 ng/ml human TNF (Invitrogen, PHC3011) and 1 ng/ml IL-6 (Invitrogen ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Immunology 2021Quote: ... Immune mediator levels in COVID-19 patient plasma across different active and convalescent groups were measured with 24-plex Human ProcartaPlex™ (ThermoFisher Scientific). The kit analyte detection panel included brain-derived neurotrophic factor (BDNF) ...
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bioRxiv - Molecular Biology 2022Quote: Keratinocytes were transfected with either HOXC13-AS siRNA or control siRNA for 24 hours (n=3 per group) and transcriptomic profiling was performed using human Clariom™ S assays (ThermoFisher Scientific) at the core facility for Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged goat anti-mouse IgG or Alexa Fluor 750-tagged goat anti-rabbit IgG (1:100, Thermo Fisher, cat# A21235 and A21039), or Alexa Fluor 647-tagged donkey anti-mouse IgG ...
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bioRxiv - Cell Biology 2019Quote: ... we transfected one 100 mm plate of DLD1 BRCA2-/- cells at 70% of confluence with 10 µg of a plasmid containing human EGFP-MBP-tagged BRCA2 cDNA (corresponding to accession number NM_000059) using TurboFect (Thermo Fisher Scientific), 48h post-transfection the cells were serial diluted and cultured in media containing 1 mg/ml G418 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... StrepII- and MBP-tagged human PUM1 and PUM2 RD and RBD constructs were expressed in E.coli BL21 (DE3) Star cells (Thermo Fisher Scientific). Cells were grown in LB overnight at 37°C ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with plasmid encoding Myc-tagged human NLRP3 using the Lipofectamine™ 3000 transfection kit (L3000015, Thermo fisher). At 24 h after transfection ...
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bioRxiv - Biophysics 2022Quote: ... FLAG-tagged human DNA2 nuclease-deficient (D277A) and helicase-deficient (K654R) mutants were generated by Phusion site-directed mutagenesis (Thermo Fisher) of wild-type DNA2-FLAG (pIF494 ...
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bioRxiv - Cell Biology 2023Quote: Methods used for these assays have been described.28 Briefly, human FLAG-tagged CDKL5 WT or kinase dead (KD, CDKL5 K42R) constructs were subcloned into pT7CFE1-CHis plasmid (Thermo Fisher). These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-tagged WT or kinase dead (KD, CDKL5 K42R) constructs of human CDKL5 were subcloned into a pT7CFE1-CHis plasmid (Thermo Fisher). A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Assay mixes with 1 nM GST-tagged GR (aa 521-777, Invitrogen #PV4689), 25 nM Alexa-488 conjugated GST antibody (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... followed by fluorescence tagged goat anti-rabbit IgG Alexafluor 488 (1:200, Invitrogen) and fluorescence tagged goat anti-mouse IgG Alexafluor 594 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa 555 or Alexa 594 tagged secondary antibodies (Invitrogen, Germany, dilution 1:2000) were used ...
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bioRxiv - Neuroscience 2022Quote: ... and Alexa Fluor 647-tagged donkey anti-rabbit IgG (1:500, Thermo Fisher, catalog # A-31573 ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa 488 or Alexa 594 tagged secondary antibodies (Invitrogen, Germany, dilution 1:1000) were used ...
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bioRxiv - Microbiology 2021Quote: ... 10% HI FBS (Gibco), 1X ultraglutamine (Lonza) ...
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bioRxiv - Microbiology 2021Quote: ... His (ThermoFisher, MA1-21315), FLAG (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... HI FBS (2273356P, Gibco). HEPES (54457 ...
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bioRxiv - Immunology 2020Quote: ... or anti-His (Invitrogen) for recombinant ZVrecE80 antigen in 0.1M carbonate buffer pH 9.6 ...
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bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
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bioRxiv - Microbiology 2022Quote: ... anti-penta HIS (Invitrogen) and anti-HSP70 (26 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Neuroscience 2024Quote: ... mass spectrometer equipped with a FAIMSpro differential ion mobility device that was coupled to an UltiMate 3000 (Thermo Scientific). Samples were loaded onto a precolumn (Acclaim 5µm PepMap 300 µ Cartridge ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) plus 2% penicillin-streptomycin (Gibco) at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...