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Citations for Thermo Fisher :
1 - 50 of 9978 citations for Recombinant Human Granzyme B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
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bioRxiv - Immunology 2020Quote: ... Granzyme B (GB12, Invitrogen), IFN-γ (XMG1.2) ...
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bioRxiv - Immunology 2022Quote: ... anti-Granzyme B (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... granzyme B (PE; GB12; Invitrogen), CD69 (PE-CF594 ...
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bioRxiv - Immunology 2021Quote: ... and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher. Cell counting was performed with CountBright Absolute Counting Beads (Thermo Fisher ...
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bioRxiv - Immunology 2019Quote: ... and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher. Antibodies against human CD62L (clone DREG-56) ...
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bioRxiv - Bioengineering 2020Quote: ... Antibodies for Granzyme B (GB12; ThermoFisher), CD69 (FN50 ...
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bioRxiv - Immunology 2021Quote: ... anti-granzyme B-AF647 (GB11, Invitrogen), anti-granzyme K-FITC (GM26E7 ...
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bioRxiv - Immunology 2021Quote: ... granzyme B (clone GB11, Invitrogen, GRB04), CD69 (clone H1.2F3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Granzyme B (Invitrogen, Cat. #MA1-35461), CD4 (Leica ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant 6x-His tagged PTB was purified using Ni-NTA agarose (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Granzyme B (1:100; clone GB11 ThermoFisher); and Perforin (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Granzyme B and Ki67 (Thermo Fisher Scientific). Cells were analyzed using a Fortessa X-20 cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... RORγt and granzyme B (GB11) from ThermoFisher Scientific.
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bioRxiv - Immunology 2020Quote: ... was purchased from Biolegend and anti-granzyme B (GB11) from ThermoFisher-Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Granzyme B (clone: 23 H8L20, Thermofisher Scientific), CD103 (clone ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining for Granzyme A and Granzyme B was performed using permeabilization buffer (Invitrogen) following fixation ...
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bioRxiv - Genomics 2022Quote: ... Granzyme B (GZMB, 1:100, Invitrogen, MA1-80734), or Granzyme K (GZMK ...
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bioRxiv - Immunology 2022Quote: ... Granzyme B PE-Texas red (clone GB11, Invitrogen), CD11b APC-eFluor780 (clone M1/70 ...
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bioRxiv - Immunology 2022Quote: ... anti-Granzyme B (Invitrogen, ThermoFisher Scientific, Langenselbold, Germany), and anti-ZAP70 (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... and APC-anti-Granzyme B (GB11; GRB05 Invitrogen), washed using perm buffer ...
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bioRxiv - Immunology 2023Quote: ... Granzyme B PE-Texas red (clone GB11, Invitrogen), CD11b APC-eFluor780 (clone M1/70 ...
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bioRxiv - Cell Biology 2019Quote: Purified recombinant human p38α (MAPK14, GST-tagged, Thermo Fisher Scientific, #PV3304), p38β (MAPK11 ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant GST-tagged kinase active human mTOR (Cat. PV4753, ThermoFisher) was used instead of the immunoprecitated mTORC1 ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with an anti-human Granzyme B antibody (10µg/ml, clone GB11, Thermo Scientific) followed by a goat anti-mouse IgG Abberior Star 580 (Abberior Instruments) ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Immunology 2020Quote: ... Antibody against granzyme B (GB11) was obtained from Invitrogen. All antibodies were tested with their respective isotype controls ...
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bioRxiv - Immunology 2021Quote: ... Granzyme B- allophycocyanin (APC) (clone GB12, Cat#MHGB05, Invitrogen), and Ki67-Alexa Fluor-700 (clone B56 ...
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bioRxiv - Immunology 2021Quote: ... Thy-1.1 (HIS51) and Granzyme B (NGZB) from Thermofisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Alexafluor-594 tagged recombinant cholera toxin subunit B (CTB-red) was also purchased from Invitrogen Molecular Probes and used as per manufacturer’s recommendations (10 µM final concentration).
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bioRxiv - Molecular Biology 2023Quote: GST-tagged p107 constructs loaded on beads were phosphorylated with recombinant cyclin B/CDK1 (Invitrogen) in 200 nM ATP KAS buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Granzyme B (effector T cells, GRB-7, Thermo Fisher Scientific), CD68 (macrophages ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... and anti-Granzyme B (clone GB12 in APC, from Invitrogen Cat# MHGB05). For all flow cytometry stainings ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking supernatant samples from different groups and standards (recombinant human-TREM2-His; Life Technologies) were incubated for 2 h at room temperature (RT ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
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bioRxiv - Cell Biology 2019Quote: ... p38β (MAPK11, His-tagged, Thermo Fisher Scientific, #PV3679), p38γ (MAPK12 ...
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bioRxiv - Cell Biology 2019Quote: ... p38γ (MAPK12, His-tagged Thermo Fisher Scientific, #PV3654), p38d (MAPK13 ...
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bioRxiv - Cell Biology 2019Quote: ... p38d (MAPK13, His-tagged, Thermo Fisher Scientific, #PV3656) and hPI31 (UBPBio ...
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bioRxiv - Immunology 2023Quote: ... 6x His Tagged monoclonal antibody (Thermo Fisher Scientific) was also plated as an experimental control ...
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bioRxiv - Microbiology 2021Quote: ELISA assays were performed in 96-microwell plates coated with 150 ng/well of SARS-CoV-2 Spike (aa 16-1213) His-tagged recombinant protein (ThermoFisher) and 1010 phage or AAVP particles/50 μL of PBS O.N ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then purified by immobilized metal affinity chromatography by using HisPur Ni-NTA (Purify His-tagged recombinant fusion proteins using high-capacity nickel-nitrolotriacetic acid methal-chelate beads) spin columns (ThermoFisher). Recombinant proteins were refolded on the column by gradually removing urea until reaching to a concentration 0 M and then dialyzed against PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Modified pBAD/His B vector (Invitrogen) containing native dnrK and tamK31,39 were used as templates for chimeragenesis ...
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bioRxiv - Immunology 2019Quote: ... with anti-IFNγ and anti-granzyme B (XMG1.2, NGBZ, eBioscience, Thermo Fisher Scientific) for 45 min ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...