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Citations for Thermo Fisher :
701 - 750 of 6379 citations for Recombinant Human GFPT1 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... and 1 μL of RNaseOut™ Recombinant RNase Inhibitor (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant SRPK1 and PKR were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant clones were selected with 120 μg/ml Hygromycin (Invitrogen) and pooled to obtain a stable population maintained in medium containing Puromycin ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... 200 U/mL RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher, 10777019), 400U/mL RNasin (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 ng/mL recombinant bFGF (Thermo Fisher Scientific, PHG0261). Human iPSCs were converted to EPS cells referred to protocol described previously16 ...
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bioRxiv - Cell Biology 2021Quote: Recombinant constructs were expressed in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media ...
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bioRxiv - Biophysics 2020Quote: The recombinant tubulin was expressed in HighFive cells (Life Technologies) and purified by three steps of column chromatography (DEAE Sepharose ion exchange chromatography ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transfection with purified plasmid using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... cells were pretreated with mouse recombinant IFN-γ (BMS326, Invitrogen) at 6.25ng/mL for overnight and followed by infection with Mycobacterium tuberculosis ...
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bioRxiv - Immunology 2021Quote: ... cells were pretreated with recombinant IL-13 (PMC0134, Thermo Scientific) at 20ng/mL and IL-4 (PMC0045 ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with recombinant murine EGF (100 ng ml−1, Gibco), recombinant human R-spondin 1 (200 ng ml−1 ...
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bioRxiv - Immunology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 ng/ml recombinant mouse EGF (Thermo Fisher Scientific, PMG8041), 1 ug/ml recombinant human R-spondin1 (R&D ...
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bioRxiv - Neuroscience 2022Quote: ... AlexaFluor™ 555 cholera toxin subunit B (recombinant) (Invitrogen #C22843)] of 1mg/mL (Molecular Probes® ...
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bioRxiv - Microbiology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with recombinant bacmids using Cellfectin II reagent (Gibco-Thermo Fisher Scientific™ ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with recombinant murine EGF (100 ng mL-1, Gibco), recombinant human R-spondin 1 (200 ng mL-1 ...
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bioRxiv - Physiology 2022Quote: Recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transient transfection ...
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bioRxiv - Microbiology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore) ...
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bioRxiv - Biochemistry 2023Quote: ... 80 U of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher), and 25 μl of T7 RNA polymerase (lab made) ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.15 μL recombinant RNase Inhibitor (40 U/μL, Thermo Fisher); 0.06 μL Dithiothreitol (DTT ...
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bioRxiv - Genomics 2023Quote: ... 200 units/mL RNaseOUT™ Recombinant Ribonuclease Inhibitor (#10777019; ThermoFisher), and 1X cOmplete ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µg (60 pmol) of recombinant Cas9 protein (A36498, Invitrogen) was incubated with 180 pmol of sgRNA in a 20 µL reaction for 20 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... 0.5 μl of RNaseOUT Recombinant Ribonuclease Inhibitor (ThermoFisher, 10777-019) to the hexamer annealed RNA ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng/mL recombinant mouse interleukin-3 (Gibco PMC0031).
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bioRxiv - Biophysics 2023Quote: ... Tetravalent recombinant (wild-type) streptavidin was procured from Thermo Scientific. Plasmids encoding SBD2 (T369C/S451C ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
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bioRxiv - Immunology 2024Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher, Cat. #A14635) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 50 ng/ml mouse recombinant EGF (Invitrogen, PMG8043). The organoids were trypsinized with 0.05% Trypsin-EDTA (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Cell Biology 2020Quote: ... The RNA transcripts were tagged with poly(A) tail using Poly(A) Tailing Kit (Invitrogen) to extend the stability of mRNA in zebrafish embryos.
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibody incubations were for 1 hour at RT using fluorescently tagged antibodies from Invitrogen. All antibody dilutions were made in a solution of 1 mg/ml BSA in 0.25% Tween 20 in PBS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... For each sample pool we retained only biotin-tagged fragments using Dynabeads M-270 (Invitrogen), and performed six separate ...
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bioRxiv - Cancer Biology 2022Quote: Myc-tagged LCK containing plasmid was generated using pENTR/D-TOPO cloning kit (Thermo Scientific) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: Biotinylated proteins were tagged using streptavidin-coated quantum dots (Qdot 655 and Qdot 605 - ThermoFisher) by incubating at 100 nM in HSABC (50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Affinity separated FLAG-tagged protein was eluted using 35 μL IgG elution buffer (Thermo Fisher), denatured with equal volume of Laemlli buffer ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with FLAG-tagged OGA using Lipofectamine 3000 reagent (Thermo Fisher Scientific) at a ratio 1:2 (μg:μl ...
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bioRxiv - Cell Biology 2021Quote: Mouse hemagglutinin (HA) tagged Lphn2 construct was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) and cloned into the Gateway system pENTR221 vector (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... HA-tagged NPH3 was immunoprecipitated using Pierce™ Anti-HA Magnetic Beads (Thermo Fisher Scientific). Thiophosphorylation was visualised by immunoblotting with anti-thiophosphoester monoclonal antibody (clone 51-8 ...
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bioRxiv - Biochemistry 2022Quote: ... and transfected with pEGFP-C3 to express GFP-tagged S142A ExoU and RFP-Lamp1 (ThermoFisher), when 70% confluent ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged RRs were purified from clarified lysate using HisPur Ni-NTA resin (Thermo Scientific) or HisPur Cobalt resin (Thermo Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Then 200-fold MPBS/MTCS-diluted secondary antibody AlexaFluor488-tagged donkey anti-rat IgG (Thermofisher) antibody for JIM5 and JIM7 ...
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bioRxiv - Neuroscience 2019Quote: ... 500 µg of proteins were used for c-Myc-tagged tomosyn co-immunoprecipitation (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... We immunoprecipitated GFP-tagged RBBP4 and RBBP7 proteins with anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each C-terminally HA tagged GC-C variant was synthesized via gene synthesis (Life Technologies). GC-C was then cloned into the lentiviral transfer vector pUltra (Addgene #24129 ...
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bioRxiv - Molecular Biology 2021Quote: Cells with tagged RPAC1 were grown in suspension in Expi293 Expression Medium (Thermo Fisher Scientific) up to 7 x 106 cells/mL ...
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bioRxiv - Biochemistry 2022Quote: ... TAP- or FTpA-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific) for 2.5 h at 20°C ...
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bioRxiv - Cell Biology 2022Quote: L6 rat myoblasts expressing myc-tagged GLUT4 (33) were maintained in α-MEM (12561056, Gibco) supplemented with 10% fetal bovine serum and 1% pen/strep antibiotic in a humidified atmosphere containing 5% CO2 and 95% air at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... or ALS-associated Ruby-tagged sequences of DnaJC7 using 5 μL of Lipofectamine 2000 (Gibco) transfection reagent in OptiMEM (Gibco ...