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Citations for Thermo Fisher :
101 - 150 of 8539 citations for Recombinant Human FZD5 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco and used at the indicated concentrations ...
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bioRxiv - Systems Biology 2021Quote: ... anti-human FITC-IgG (Fc specific) (ThermoFisher Scientific) at a dilution of 1:400 and for 45 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 100ug/mL anti-human IgG(Fc) (Life Technologies) was added to virus-Ab complexes and incubated for 30 min at 37°C prior to the addition of cells as described in (39) ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10 % V/V heat inactivated Fetal Calf Serum (Hi FCS, Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (hi-FCS) (Gibco) and 1% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... medium containing 10% (v/v) heat inactivated fetal calf serum (HI-FCS; Gibco), 100 U/mL Penicillin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10 % v/v heat-inactivated fetal calf serum (HI-FCS, Gibco), 1 mM non-essential amino acids (NEA ...
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bioRxiv - Cell Biology 2021Quote: All FZD5 and FZD6 amber mutants were generated using the GeneArt™ Site-directed Mutagenesis System (Thermo Fisher Scientific) with the following primers ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged RRs were purified from clarified lysate using HisPur Ni-NTA resin (Thermo Scientific) or HisPur Cobalt resin (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr: 11533923, Fisher Scientific), a HRP-coupled secondary antibody (ArtNr ...
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bioRxiv - Neuroscience 2023Quote: ... The His-tagged dAsapPZA protein was expressed and purified following the manufacturer’s guidelines (Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... His-tagged LCRIS_00661 and LCRIS_00558 genes with the appropriate restriction sites were ordered from Invitrogen and subcloned into a previously constructed S ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml recombinant human GDNF (Invitrogen, USA) and 1% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: ... 20 ng/mL human recombinant EGF (Thermo Fisher), 10 ng/mL cholera toxin ...
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bioRxiv - Immunology 2022Quote: ... 50 ng/mL recombinant human EGF (Gibco, PHG0314), 80 ng/mL recombinant human R- spondin 1 (R & D systems ...
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bioRxiv - Developmental Biology 2019Quote: ... BMP2 recombinant human protein (Thermo Fisher Scientific, #PHC7145) was applied for 1h on cells ...
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bioRxiv - Neuroscience 2021Quote: Human recombinant IGF1 was purchased from Thermo Fisher, and K252a ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10 Units/mL human recombinant insulin (Gibco). All cell culture was conducted in a class two ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant full-length human G2019S-LRRK2 protein (Invitrogen) was used as a protein standard ...
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bioRxiv - Cancer Biology 2020Quote: ... Human recombinant EGF was from Invitrogen (cat # PHG0311) or PeproTech (cat # AF-100-15) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human bFGF (Cat# 13256029) purchased from Gibco; LY294002 (Cat# PHZ1144 ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg/mL human recombinant insulin (Thermo Fisher), and 1 μg/mL hydrocortisone (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Human BMP-4 Recombinant Protein (Gibco, Catalog # PHC9534). For cell culture of iPSCs on niches ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-8 Monocyte Recombinant Human Protein (ThermoFisher, PHC0884), and Recombinant Human CXCL1/GROα Protein (R&D Systems ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant human insulin was purchased from Thermo Fisher USA ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10 IU/ml human recombinant insulin (Gibco). OAW28 was grown in Advanced DMEM (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 ng/mL human recombinant EGF (Gibco, #PHG0314), 20 ng/mL human recombinant FGF (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Vitronectin (recombinant human protein) was from Fisher Scientific.
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bioRxiv - Cancer Biology 2020Quote: ... 20 ng/mL recombinant human EGF (Gibco #PHG0313), 10 ng/mL basic-FGF (Millipore #GF003-AF) ...
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bioRxiv - Neuroscience 2020Quote: ... human recombinant brain-derived neurotrophic factor (Life Technologies), penicillin/streptomycin ...
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bioRxiv - Cell Biology 2019Quote: ... 10 ng/mL recombinant human EGF (Thermo Fisher), 25 ng/mL prostaglandin E1 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng/ml human recombinant EGF (Gibco, #PHG0311L), 100 ng/ml Cholera Toxin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/mL human recombinant EGF (ThermoFisher PHG0311), Antibiotic-Antimycotic (Gibco 15240-062) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human IL-2 was purchased from ThermoFisher Scientific.
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bioRxiv - Neuroscience 2022Quote: ... 10μg ml−1 human recombinant insulin (GIBCO, 12585014).
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bioRxiv - Cell Biology 2023Quote: ... exposed to recombinant human TGFβ1 (Thermo Fisher 7754BH005) 2ng/ml for 3 days ...
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bioRxiv - Biophysics 2023Quote: ... The recombinant human TGF-β1 (Invitrogen, MD, USA) was added at a final concentration of 1 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... human recombinant laminin (0.2 mg/ml, Life Technologies), Y-27632 (10 mM ...
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bioRxiv - Developmental Biology 2023Quote: ... 12.5 µg/mL human insulin recombinant zinc (Gibco), 20 ng/mL leukaemia inhibitor factor (Cambridge Stem Cell Institute) ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant Human EGF (Invitrogen, 100 μg/500 mL), Hydrocortisone (Sigma H-0135 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10ng/ml recombinant human IL-3 (Gibco). Cultured cells were infected with lentivirus encoding GFP/Scrambled control shRNA overnight and fresh media was added the following day ...
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bioRxiv - Cell Biology 2024Quote: ... 1.61 nM EGF Recombinant Human Protein (Invitrogen, PHG0313), 1.1 μM hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Biophysics 2020Quote: ... and inserted into a human Fc expression vector (Invitrogen). The proteins were expressed in HEK293T cells ...
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bioRxiv - Synthetic Biology 2021Quote: ... Sybr Safe DNA gel stain and BenchMark™ His-tagged Protein Standard were purchased from Invitrogen. HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech ...
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bioRxiv - Evolutionary Biology 2020Quote: ... His-tagged proteins were eluted from the beads in a disposable polypropylene column (29924, ThermoFisher Scientific) using elution buffer (4 ml wash buffer containing 400 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... media was collected and His tagged proteins were bound using HisPur Ni-NTA Resin (ThermoFisher; 88221) in the presence of Calbiochem’s EDTA-free protease inhibitor cocktail 1:1000 (MilliporeSigma ...