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Citations for Thermo Fisher :
501 - 550 of 6421 citations for Recombinant Human FGF2 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and a 546-nm fluorescently tagged Protein A (ThermoFisher, P11049) as our linking protein ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated with appropriate fluorescent-tagged secondary antibodies (1:250, Invitrogen) for two hours at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... and a Cy5-tagged secondary antibody (1:2000, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... APEX2-tagged hiPSCs were washed with DPBS (Thermo Fisher, 14190136) and then incubated in DPBS containing 0.5 mM biotin-phenol (Iris Biotech ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were visualized using Pierce ECL (Thermo Scientific) after treatment with an HRP-conjugated primary antibody directed against the FLAG epitope (Millipore Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells with either ATF6 plasmids or ATF6-mutant plasmids were transfected with 200 ng of FGF2-ERSE-luciferase-reporter plasmids using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... ESCs cultured on fibronectin-coated plates in 2i/LIF medium were exchanged with serum-free N2B27 with Activin A and FGF2 as described above with the addition of 1% KnockOut Serum Replacement (Gibco). EpiSC-L cells are collected for analysis after 10 days of differentiation.
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged goat anti-mouse IgG or Alexa Fluor 750-tagged goat anti-rabbit IgG (1:100, Thermo Fisher, cat# A21235 and A21039), or Alexa Fluor 647-tagged donkey anti-mouse IgG ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/mL recombinant murine EGF (Gibco, PMG8043), 100ng/mL recombinant human Noggin (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/ml recombinant EGF (Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
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bioRxiv - Immunology 2022Quote: ... The recombinant ORF8 (ThermoFisher, Cat. RP-87666) was conjugated with FITC using the FITC conjugation kit (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) was added to lysate (1ul per 100ul lysate) ...
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bioRxiv - Genomics 2019Quote: ... 0.25 μL RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen), was then added to each well ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant Cas9 2.0 (Thermo Fisher Scientific, A36498) was mixed with gRNA and incubated for 15 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant LRRK2 protein was from ThermoFisher (#A15197). For pT73 Rab10 assay ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant pSinRep5 and helper plasmid pDH26S (Invitrogen) were transcribed in vitro into capped RNA using the Megascript SP6 kit (Ambion) ...
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bioRxiv - Microbiology 2022Quote: ... and 0.2 ng/mL recombinant EGF (Gibco). The transfection of primary NHKs to generate HPV18+ keratinocytes was performed as described previously [86] ...
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bioRxiv - Molecular Biology 2022Quote: ... RNaseOUTTM Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) and Protoscript II reverse transcriptase (New England BioLabs ...
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bioRxiv - Physiology 2022Quote: ... Recombinant PIK3CG was purchased from Thermo Fisher.
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant TrueCut Cas9 V2 (Thermo Fisher Scientific) was diluted in resuspension buffer R ...
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bioRxiv - Immunology 2024Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (10777019, Invitrogen); Deoxynucleotide (dNTP ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.2 ng/mL recombinant EGF (Gibco). HPV18+ NHKs were generated as described previously [86] ...
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bioRxiv - Cell Biology 2024Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher), Random Hexamer Primers (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... lectin PNA tagged with Alexa Fluor 488 (Thermo Fisher Scientific Inc.) was used.
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bioRxiv - Molecular Biology 2020Quote: ... GST-tagged NgAgo variants were purified by glutathione agarose (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 100 ng/mL pHrodo tagged zymosan A beads (Thermo Fisher Scientific), 100 ng/mL pHrodo tagged S ...
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bioRxiv - Evolutionary Biology 2019Quote: ... for His-tagged proteins or GST-agarose resin (Thermo Fisher Scientific) for GST-tagged proteins ...
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bioRxiv - Cell Biology 2019Quote: ... HA-tagged proteins were eluted by incubation with HA peptide (ThermoFisher) for 2 × 15 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: To localize GFP-tagged fusion proteins with BODIPY Red (Molecular Probes), transfected cells were grown overnight (16-20h ...
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bioRxiv - Cell Biology 2020Quote: ... To localize GFP-tagged fusion proteins with MitoTracker Red (Molecular Probes), after 16h transfection ...
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bioRxiv - Microbiology 2021Quote: ... for FLAG® (DYKDDDDK)-tagged proteins in iBind Western System (Invitrogen). Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2019Quote: ... tagged proteins were eluted and quantified by BCA (Pierce, ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Myc-tagged proteins were immunoprecipitated with Protein G Dynabeads (Invitrogen, 10003D) coated with anti-myc antibody (Cell Signaling ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated in Alexa tagged secondary antibodies (Life Technologies, Invitrogen) prepared in SG-PBS for 1hr at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated in Alexa tagged secondary antibodies (Life Technologies, Invitrogen) prepared in SG-PBS for 1hr at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Tagged proteins were visualized using chemiluminescent reagents (Thermo Fisher Scientific 34579).
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bioRxiv - Neuroscience 2023Quote: ... and AlexaFluor 488-tagged donkey-anti-mouse (Molecular Probes, 1:1000) secondary antibodies and Hoescht 33342 stain (Molecular Probes ...
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bioRxiv - Neuroscience 2023Quote: ... and AlexaFluor 647-tagged donkey-anti-rabbit (Molecular Probes, 1:1000) secondary antibodies for one hour ...
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bioRxiv - Cell Biology 2023Quote: ... The RFP-tagged organelle markers were purchased from Invitrogen (Carlsbad, CA): CellLight Golgi-RFP BacMam 2.0 (Cat# C10593) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3mM CaCl2 with 250 units of 6xHIS-tagged SUMO protease (ThermoFisher) for scar-free SUMO tag cleavage ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The tagged cDNA fragments were subcloned into expression vector pcDNA3.1(+) (Invitrogen). Transient transfection of HEK293 cells by plasmid DNA was carried out by Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria are tagged with FM-4-64 dye (Thermo Scientific). In the confocal microscope study ...
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bioRxiv - Biophysics 2024Quote: ... Nitrocellulose membrane was incubated with His6-tagged Monoclonal Antibody - HRP (Invitrogen) with 1:3000 v/v dilution in PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then stained with fluorescently tagged secondary antibodies (Thermo Fisher Scientific Cat# A10037 ...
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bioRxiv - Bioengineering 2023Quote: ... 70 E8 media was aspirated and replaced with a Differention Media cocktail designed to stimulate cortical neurogenesis via the removal of FGF2 consisting of: DMEM/F12 (48.25%; Gibco 11330-032), Neurobasal-A (48.25% ...
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bioRxiv - Developmental Biology 2023Quote: ... were maintained in primed state using MEF-conditioned media (CM) supplemented with FGF2 (20ng/mL) on Geltrex coated dishes (ThermoFisher Scientific). CM-FGF2 media was refreshed every day ...
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bioRxiv - Neuroscience 2023Quote: ... and then into NPCs (Neural Progenitors) in NPC medium (N2B27 medium with supplements and with additional 20 ng/mL FGF2 (Gibco; PHG0021) and 1 ug/mL Laminin (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... Then recombinant dsDNA fragment (add to > 400 ng/μl final concentration) or recombinant single-strand DNA (ordered from Invitrogen or IDT ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...