Labshake search
Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Recombinant Human F3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
-
bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
-
bioRxiv - Molecular Biology 2021Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
-
bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
-
bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
-
bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
-
bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
-
bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
-
bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
-
bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 Spike RBD 319-541 recombinant protein (OBA0101, Ovodan Biotech) was expressed in 25 mL culture using the Expi293F expression system (#A14635; ThermoFisher Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
-
bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
-
bioRxiv - Biochemistry 2023Quote: Recombinant trimeric spike proteins (5 µg/mL) were plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
-
bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
-
bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
-
bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
-
bioRxiv - Cell Biology 2020Quote: ... 25ng/mL recombinant murine EGF (Gibco), 50ng/mL recombinant human HGF (Peprotech) ...
-
Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... and RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... we added recombinant mouse IFNγ (ThermoFisher), 2-deoxyglucose (Abcam) ...
-
bioRxiv - Microbiology 2022Quote: ... and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). Next ...
-
bioRxiv - Cancer Biology 2020Quote: ... Recombinant IGF1 (Life Technologies, cat# PHG0071) and picropodophyllin (PPP ...
-
bioRxiv - Genomics 2019Quote: ... 50ng/mL recombinant murine EGF (Gibco), and 100ug/mL recombinant human Noggin (Peprotech) ...
-
bioRxiv - Cell Biology 2023Quote: ... The recombinant vitronectin (A14701SA, Thermo Fisher) was diluted in DPBS at the concentration of 1:200 to get the final concentration of 2.5 mg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... Recombinant TNFα (Thermo Fisher, cat# PHC3015) was reconstituted at 0.1mg/ml in water ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse recombinant Egf (Life Technologies #PMG8043), 100 ng/mL mouse recombinant Noggin (Peprotech #250-38 ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
-
bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
-
bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
-
bioRxiv - Immunology 2022Quote: ... The secreted fusion protein was purified on a CaptureSelect™ human albumin affinity matrix (Life Technologies), and protein eluted by adding 20 mM Tris and 2.0 M MgCl2 ...
-
bioRxiv - Genomics 2022Quote: ... human preadipocytes in a 6-well plate were transfected with 6.25ug Cas9 protein (Fisher Scientific, A36498) and 800ng sgRNAs (IDT) ...
-
bioRxiv - Microbiology 2023Quote: Human ACE2 fusion proteins were obtained from transient transfection using the Expi293TM system (Thermo Fisher Scientific). All plasmids used in this study were generated by ATUM Bio and transient transfection was carried out following the manufacturer’s protocol for the ExpiFectamineTM 293 transfection kit (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
-
bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
-
bioRxiv - Microbiology 2019Quote: ... 125ng of total recombinant protein was separated on 4-20% or 10-20% Novex WedgeWell Tris-Glycine precast gels (Thermofisher, Waltham, MA). For silver stain ...
-
bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
-
bioRxiv - Immunology 2020Quote: ... Plates were then incubated with 1μg/ml of biotinylated recombinant ACE2 protein for 1 hour at room temperature followed by incubation with HRP-conjugated streptavidin (Thermo Fisher Scientific) for 1 hour at room temperature ...
-
bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.4 picomol of folded reporter mRNA was mixed with 20 picomol of recombinant protein and 200 picomol of UltraPure BSA (Thermo Fisher # AM2618) in binding buffer (10 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2022Quote: ... 28 fmol of folded reporter mRNA was mixed with 0-10 picomol of recombinant protein and 100 picomol of UltraPure BSA (Thermo Fisher # AM2618) on ice for 1 hr ...
-
bioRxiv - Microbiology 2023Quote: Concentration of recombinant proteins were determined by measuring absorbance at 280 nm using a NanoDrop 1000 and/or NanoDrop One (Thermo Fisher Scientific) considering the extinction coefficient and the molecular weight calculated with the ProtParam tool (http://www.expasy.org/tools/protparam.html).
-
bioRxiv - Immunology 2023Quote: ... the microtiter plates were washed 5 × with PBS-T and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of both bat and human IgG antibodies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
-
bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
-
bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...