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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Recombinant Human ERBB2 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled anti-human IgG Fc antibody (Catalog# 31413, Thermo Fisher) was added to each well and incubated for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... nanovials were modified with biotinylated goat anti-human IgG Fc antibody (Thermo Fisher Scientific). Samples were resuspended in DMEM and seeded in a well plate ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated Abs specific for the Fc region of human IgG (Invitrogen) were used as secondary antibodies to detect antibody binding in ELISA experiments ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Protease susceptibility measurements were performed in biological replicates (n = 2 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Titrations were performed in biological replicates (cultures from independent colonies grown on separate days ...
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bioRxiv - Immunology 2020Quote: ... followed by addition to the Fc-chimeric human ACE2-coated MaxiSORP ELISA plate (Nunc) for an hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Human JAGGED1-Fc (Martin et al., 2023) was transfected into Expi293F cells (ThermoFisher, A14527) using FectroPro (Polyplus ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human Fc chip was regenerated to baseline using IgG Elution Buffer (Thermo Scientific) and the process was repeated for all humAbs in the panel ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cardiomyocytes were labeled with of DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17, Invitrogen, MA1-142-A488) at 1:500 dilution in 1% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... The protein was labeled with Rv3718c was labeled with Alexa Fluor 647 NHS Ester (Invitrogen™ Catalog# A20106) for 30 minutes at room temperature in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... The purified GlaS proteins were labeled using a FITC protein labeling kit (F6434, Thermo Fisher) and their protein concentrations were determined by Bradford assay.
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bioRxiv - Cell Biology 2022Quote: Recombinant protein concentration was quantified through BCA protein assay kit (Thermo Fisher Scientific, Buenos Aires, Argentina) on a BioRad 3350 Microplate Reader using BSA as standard ...
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bioRxiv - Immunology 2023Quote: ... The concentrations of the purified recombinant proteins were determined by the BCA protein assay kit (ThermoFisher) and separated by reducing SDS-PAGE and visualized by silver staining ...
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bioRxiv - Immunology 2022Quote: ... The concentrations of the purified recombinant proteins were determined by BCA protein assay kit (Thermo Scientific) using bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RBD-hFc proteins were captured by Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), washed by wash buffer (100 mM Tris/HCl ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... SV5-tagged proteins were detected using mouse anti-SV5 mAb (1:5000, Life Technologies); HA-tagged proteins were detected by anti-HA mAb (clone HA-7 ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher). To eliminate nonspecific binding proteins ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
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bioRxiv - Immunology 2022Quote: ... for viability and antibodies directed against human CD19 (APC clone SJ25-C1, Thermo Fisher Scientific), CD38 (PE-Cy7 clone HB-7 ...
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bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were labeled with an anti-human AlexaFluor 488-conjugated secondary antibody (Invitrogen) at 1:500 and labelled with HCS Cell Mask TM Blue Stain (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... mono-Fc was removed using Pierce Protein A agarose beads (Thermo Scientific). The flow-through containing PvDBP-RII was then mixed with DB1 or DB9 Fab (1.5:1 Fab to protein ratio ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: Recombinant 6XHis-tagged TbVps24 (N-terminus tag) was cloned using the pTrcHis TOPO TA expression system (Thermo Fisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Human protein arrays (ProtoArrays V5.0, Life Technologies) kept at -20°C were equilibrated at 4 °C for 15 min and ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Genetics 2019Quote: ... cells were incubated in differentiation medium (DM) (10 µg/ml recombinant human insulin (Gibco), 2% HI horse serum (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Basal Medium plus 50 ng/ml human recombinant VEGFA (PHC9391 Gibco, Frederick, MD, USA) or Basal Medium plus 100 ng/ml recombinant human NTN1 (6419-N1 ...
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bioRxiv - Cell Biology 2022Quote: ... 4h prior treatment with recombinant human epidermal growth factor (EGF) (Cat# RP-10927, Invitrogen) at 5 ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen, #35351, Lot# 1700382, 1:1000) to label EGFR.
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bioRxiv - Immunology 2023Quote: ... supplemented with 294.4 ng/l human recombinant Epidermal Growth Factor (EGF) (Gibco, 10450-013), 25 mg/l Bovine Pituitary Extract (Gibco ...
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bioRxiv - Immunology 2021Quote: Avi-tag-biotinylated spike and RBD were incubated with subsequent fluorochrome-labeled streptavidin at 4:1.5 ratio overnight at 4 °C as follows: spike with APC-streptavidin (Invitrogen), Wuhan RBD with PerCP-Cy5.5-streptavidin (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... The biotinylated proteins were tetramerized by adding APC-conjugated streptavidin (Thermo Fisher Scientific) at an allergen ...
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bioRxiv - Bioengineering 2024Quote: ... and human and mouse DKK3 (hDKK3: AA 1-350, mDKK3: AA 1-349) triple tagged with Fc-Myc-6xHis were transfected into Expi293FTM (Thermo Fisher Scientific) cells at a density of 3 × 106 viable cells/mL using ExpiFectamineTM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and Myc-tagged human Insig1 (NM_005542) were prepared by subcloning PCR products into the pcDNA3 vector (Invitrogen). YFP-tagged human RHBDL4 and YFP-human SREBP-1c-CFP were prepared by subcloning PCR products into the pCI-EYFP vector ...
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-rabbit or anti-human IgG (H+L) (Life Technologies) respectively ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
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bioRxiv - Biophysics 2023Quote: ... they were transfected with the S6-tagged human TrkA by means of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...