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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Recombinant tau monomers (human 4R2N T40/ Evotec, Germany) were labeled with Cy3 (Life Technologies). To prepare tau oligomers ...
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bioRxiv - Molecular Biology 2020Quote: Human β-cells were seeded onto collagen coated 8-well chambered cover glasses (Lab-Tek, Thermo Scientific) at a density of 70,000 cells/cm2 ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ & mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ and mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
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bioRxiv - Synthetic Biology 2020Quote: ... and the His-tagged proteins on the cell surface were then visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA) using FUSION Solo S documentation system (Vilber ...
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Developmental Biology 2021Quote: ... alpha-smooth muscle actin (1:500; Thermo Fisher Scientific, MA1-06110), ACE2 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... Anti IL-1 alpha (Thermo Fisher Scientific; Cat.# 11-7118-81).
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bioRxiv - Cancer Biology 2022Quote: ... PE-Cyanine7_TNF alpha (1:100, Thermo Fisher Scientific, #25-7423-82)
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4 °C and alpha-Tubulin (Invitrogen, 11224-1-AP) for 1-2 hours at room temperature ...
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bioRxiv - Pathology 2019Quote: ... anti-alpha smooth muscle actin antibody (1:200, 180186; Life technologies), anti-CD31 (1:100 ...
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bioRxiv - Bioengineering 2022Quote: ... 2) alpha-SMA-eFluor660 (1:150; ThermoFisher Scientific; 50-9760-82), TGF-beta-PE (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... 1/500 diluted alpha-synuclein monoclonal antibody (Syn 211, Thermofisher, AHB0261) prepared in intracellular staining buffer (ISB ...
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bioRxiv - Cell Biology 2022Quote: ... alpha-tubulin (MS-581, Thermo Fisher; 1:10,000 for western blotting) Lamin B1 (ab16048 ...
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bioRxiv - Developmental Biology 2023Quote: ... Alpha Amylase (rabbit polyclonal, 1:200; PA5-51078, Thermo Fisher Scientific), Insulin (rabbit polyclonal ...
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bioRxiv - Developmental Biology 2024Quote: ... Alpha-bungarotoxin (BTX) conjugated with Alexa Fluor 647 (1:500, Invitrogen) was used in order to visualize nicotinic acetylcholine receptor densities ...
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bioRxiv - Molecular Biology 2019Quote: ... Hepatocytes were seeded as 2.5 × 105 cells/well onto type I collagen coated 12-well plates in attachment medium (William’s E media, #12551032, Thermo Fisher Scientific) with 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: Eight-well glass plate was coated with a solution of 100 µg/ml Collagen Type I (Thermofisher Scientific, Waltham, MA, # A1064401) dissolved in 0.01N HCl and air dried ...
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bioRxiv - Cell Biology 2021Quote: ... 15 × 104 HEK 293 cells were seeded on collagen-coated coverslips (Cellmatrix, Type IV, Nitta gelatin, Osaka, Japan) and transfected with 5 nM siRNA using RNAiMax (Thermo Fisher) using a reverse-transfection protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×104 cells were seeded on top of 1.7mg/mL collagen (type I, rat tail) with or without Matrigel matrix (both from Corning, via Thermo Fisher Scientific) supplemented with 10% minimum essential medium alpha medium (MEMalpha ...
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bioRxiv - Genomics 2022Quote: ... two-thirds of the cells were collected for single-cell sequencing and the remaining cells were re-plated on 6-well plates coated with 5 μg/cm2 Type I rat collagen (Gibco, A1048301). Two additional samples were collected on Days 11 and 21 for single-cell sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... and Myc-tagged human Insig1 (NM_005542) were prepared by subcloning PCR products into the pcDNA3 vector (Invitrogen). YFP-tagged human RHBDL4 and YFP-human SREBP-1c-CFP were prepared by subcloning PCR products into the pCI-EYFP vector ...
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-rabbit or anti-human IgG (H+L) (Life Technologies) respectively ...
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bioRxiv - Biophysics 2023Quote: ... they were transfected with the S6-tagged human TrkA by means of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
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bioRxiv - Neuroscience 2020Quote: ... and collagen (ThermoFisher). Astrocytes serve as a feeder layer for neurons and were seeded (50,000/well ...
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bioRxiv - Neuroscience 2020Quote: ... CCM2 isoforms were inserted into pcDNA3.1/V5-His-TOPO and Topo pCR®II plasmid vectors (Invitrogen) respectively (1-6 ...
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bioRxiv - Molecular Biology 2019Quote: ... RING1Bfl/fl conditional mESCs were transfected with pCAG vectors encoding 2xFlag-HA-tagged mouse wild-type or RING1B I53A/S using Lipofectamine 2000 (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... mESCs were transfected with 10ug pCAG vectors encoding 2xFlag-HA-tagged BAP1 wild-type or BAP1 C91S using Lipofectamine 2000 (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... We coated glass substrates with collagen rat tail 1 (Gibco) 30 μg/ml for 2 hours ...
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bioRxiv - Pathology 2022Quote: ... and Collagen I (Thermo-Fisher Scientific, PA1-26204; 1:100). The secondary antibodies for immunofluorescence include donkey anti-rabbit Alexa Fluor 488 and donkey anti-chicken Alexa Fluor 594 (Thermo-Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Rabbit anti-collagen-IV (#PA5-104508; Thermo Fisher; 1:100), Rabbit anti-laminin (#ab11575 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mg/ml collagen solution (Thermo Fisher Scientific, Waltham, USA) and 17 mM acetic acid solution or with PLL solution (0.1 mg/ml) ...
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bioRxiv - Cancer Biology 2021Quote: ... were evenly coated with 75 µL of a 1 mg/mL collagen solution composed of 3 mg/mL rat tail collagen (Gibco), serum free media ...
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bioRxiv - Genetics 2019Quote: ... were joined with the PCR amplified wild-type or activated Tkv cytoplasmic domain in the pIB/V5-His vector (ThermoFisher). The activated Tkv chimera (Tac-TkvA ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... the muscle slurry was digested with 1000 U/mL Collagenase type II (Gibco, Cat# 17101015) in 10 mL of complete Ham’s F-10 medium (Lonza ...
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bioRxiv - Genetics 2020Quote: ... and subjected to five rounds of digestion using type II collagenase (Thermo Fisher Scientific, 17101015). Digests three to five were collected for culture ...
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bioRxiv - Cancer Biology 2021Quote: Cells grown in GelMA hydrogels were dissociated using collagenase Type II enzyme (17101015, Gibco, USA) at a concentration of 1mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... In the sequence cell suspension was dissociated by collagenase type II (Gibco™, Carlsbad, USA) under agitation at 37 °C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Hearts were explanted and digested with Collagenase Type II at 1mg/ml (Thermo Scientific, USA) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... that was immobilized in a well of a microtiter plate (type II, F96 Maxisorp, Nunc) via precoated anti-HIS antibodies in the presence of 10 μg/ml RBD-SD1-mouse IgG ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM Hepes-K+ pH 7.6) containing 2 mg/mL of collagenase type II (GIBCO) for 1 h at room temperature ...
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bioRxiv - Physiology 2021Quote: ... We performed the assay using a modified 1-plex protocol for Fast Blue Detection using Type 1 Mouse AGER probe and Type 1 Mouse GAPDH probe (Affymetrix). The sections were counterstained with Nuclear Fast Red ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated with appropriate fluorescent-tagged secondary antibodies (1:250, Invitrogen) for two hours at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... and a Cy5-tagged secondary antibody (1:2000, Thermo Fisher Scientific Inc. ...
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bioRxiv - Bioengineering 2022Quote: ... Tissue fragments were digested with type II collagenase at 5mg/ml for 1 hour at 37°C and subsequently incubated with 1X TrypLE (Gibco, Waltham, USA) for 15 minutes at 37°C ...