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Citations for Thermo Fisher :
601 - 650 of 7393 citations for Recombinant Human CSNK1G1 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 6-well plates were coated with 20 µg/mL Human recombinant laminin-521 (Biolaminin; Biolamina; LN521-05) in 1x dPBS++ (Gibco; 14040117) and incubated overnight at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... HA-tagged polyQ and Myc-tagged polyA proteins were enriched using Pierce™ Anti-HA Agarose (Thermo Fisher Scientific) and Myc-Trap®_A Kit (Chromotek ...
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bioRxiv - Microbiology 2020Quote: Dynabeads His-Tag Isolation & Pulldown (Invitrogen) were washed in PBS-TH (PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-His (Thermo Fisher scientific, rd230540a), anti-EZH2 (Cell signaling #5246 ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: ... pcDNA3.1/V5-His (Thermo Fisher Scientific), pCLXSN-GFP (Reiley et al 2005) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% HI-FBS (Gibco) at 37°C in the presence of 5% CO2 ...
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bioRxiv - Genetics 2019Quote: ... 2% HI horse serum (Thermo Fisher), and 1% P/S ...
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bioRxiv - Immunology 2021Quote: ... magnetic beads (Dynabeads His-Tag, Invitrogen) were coated with histidine tagged S protein according to the manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Hi-Di Formamide (Applied Biosystems, California) and 500 liz size standard (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... with 10% HI-FBS (Gibco, #10082147) and 1% Penicillin-Streptomycin (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... Stock anti-His antibody (Thermo Fisher) was diluted to 70 μg/mL in BRB80 buffer ...
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bioRxiv - Microbiology 2022Quote: ... Mouse-anti-His (HIS.H8) antibody (Invitrogen) (for eGFP/His-tagged NP protein co-immunoprecipitation ...
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bioRxiv - Neuroscience 2020Quote: ... containing 10% HI FBS (Life Technologies) and 35 µg/ml Ascorbic acid (Sigma) ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) was coated at 2 μg/mL in PBS onto half-area 96-well high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) or streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-Horse Serum (Gibco, 26050088) + 0.8% Pen/Strep (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Microbiology 2021Quote: Plasmid based on pBAD/His (Invitrogen) or pHT01 (MoBiTech ...
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bioRxiv - Microbiology 2020Quote: ... the pIB/V5-His vector (Invitrogen) and insert egfp fragment were amplified by PCR ...
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bioRxiv - Biochemistry 2020Quote: ... Anti‐His tag monoclonal antibody (Invitrogen) was used in western blot analysis.
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bioRxiv - Biochemistry 2021Quote: Modified pBAD/His B vector (Invitrogen) containing native dnrK and tamK31,39 were used as templates for chimeragenesis ...
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bioRxiv - Biochemistry 2021Quote: ... His (1:1000, Invitrogen, mouse monoclonal), Flag M2(1:1000 ...
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bioRxiv - Immunology 2022Quote: ... and Hi-5 cells cells (Invitrogen), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... + 10% HI-FBS (Thermo Fisher, 16140071) prior to adding indicated concentration of human IFN-alpha 2b (pbl Assay Science ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant RNase H (Invitrogen) was later added to the reverse transcribed samples and incubated for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Recombinant B18R (Life Technologies) was used at 1ug/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transfected with pDisplay encoding myc-tagged GluA1-GluA4-ATD or pCAGGS encoding FLAG-tagged Nrx or GFP with Lipofectamin 2000 (Invitrogen). On the following day ...
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bioRxiv - Microbiology 2020Quote: Human IFITM proteins and SARS-CoV-2 viral proteins were cloned into MaMTH N-term tagged Prey and C-term tagged Bait vectors respectively using Gateway cloning technology (ThermoFisher). Correctness of recombined insertions was confirmed by Sanger sequencing (Eurofins) ...
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bioRxiv - Cell Biology 2023Quote: U2OS were transfected with the MAC-tagged GTPBP8 expression vector or a control MAC-tagged GFP vector using Fugene 6 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Millipore] and isolated using Pierce GST Spin Purification Kit (Thermo Scientific). GST-p85-S6K1 expression vector48 was obtained from Addgene (Plasmid #8466 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was incubated with GST-TAG antibody (1:1000, ThermoFisher) or anti-MC4 (rabbit ...
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bioRxiv - Microbiology 2019Quote: ... GST-tag and 3xFLAG-tag fusions were achieved using pDEST27 (Invitrogen) and pCI-neo-3xFLAG vector ...
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bioRxiv - Molecular Biology 2020Quote: ... The GST tag was removed using HRV 3C protease (Thermo Scientific) with an enzyme to substrate ratio of 1:100 at 4°C for 16 hrs ...
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bioRxiv - Cell Biology 2023Quote: ... anti-GST mouse monoclonal (Invitrogen, #MA4-004, 1:5000 in WB), anti-PLK4 rabbit polyclonal (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... and polyclonal anti-GST (Thermo Fisher Scientific, Waltham, MA, USA, CAB4169) antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 500 μm disk patterns were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in pre-warmed DPBS (Thermo Fisher Scientific) for 3 hours at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... IPS cells were thawed and cultured at all times cultured on recombinant human laminin LN521 (Biolamina, 2021-21) kept in E8 basal medium (Thermo Fisher Scientific) supplemented with primocin (0.1 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were dissociated with TrypLE Express for 5 mins at 37°C and induced into EpiLCs by addition of human recombinant basic fibroblast growth factor (bFGF) (Invitrogen, 13256-029) and activin A (Peprotech ...
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bioRxiv - Cell Biology 2021Quote: ... Phages displaying human Fabs were enriched after three rounds of biopanning on biotinylated recombinant human ACE2 immobilised to streptavidin Dynabeads (Dynal M-280, Invitrogen, cat # 112.06D). After the third round of panning ...
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bioRxiv - Microbiology 2020Quote: ... To starve Chlamydia of tryptophan HeLa cells were incubated for 24 h in media containing 2 ng/ml recombinant human IFN-γ (Invitrogen: PHC4033) prior to infection ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pulsed in starvation buffer supplemented with 0.1% w/v bovine serum albumin at 4 °C for 40 min with 50 ng/mL human recombinant EGF (Thermo-Fisher, Gibco™, PHG0311L) to allow ligand bind to the EGFR ...
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bioRxiv - Cell Biology 2020Quote: ... 0.055 mM β-mercaptoethanol (#21985-023) and recombinant human full-length bFGF (#PHG0261) at 10 ng/ml (all reagents from Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... and cultured in RPMI medium supplemented with 10% FBS and differentiated for 7 days with 20 ng/mL recombinant human Macrophage-Colony Stimulating Factor protein (M-CSF) (Thermo Fisher Scientific), and 100U/L of penicillin-streptomycin solution (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
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bioRxiv - Cell Biology 2023Quote: ... DD5A) and three controls (Ctl1B, Ctl7C, Ctl8F) were cultured on human recombinant laminin LN521 (Biolamina) in Essential-8TM medium (Thermo Fisher Scientific) and passaged using Trypl Express (Thermo Fisher Scientific ...