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Citations for Thermo Fisher :
101 - 150 of 8180 citations for Recombinant Human CD3D Flag and Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific, E304050), 2.4 µM human His6-UBE2T E2 enzyme (Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Biochemistry 2021Quote: GST-tagged p107 constructs loaded on beads were phosphorylated with recombinant Cyclin A/CDK2 (Invitrogen) in 200 nM ATP KAS buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Alexafluor-594 tagged recombinant cholera toxin subunit B (CTB-red) was also purchased from Invitrogen Molecular Probes and used as per manufacturer’s recommendations (10 µM final concentration).
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bioRxiv - Molecular Biology 2023Quote: GST-tagged p107 constructs loaded on beads were phosphorylated with recombinant cyclin B/CDK1 (Invitrogen) in 200 nM ATP KAS buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Immunology 2022Quote: ... human recombinant Annexin V (5 μg/ml) (Invitrogen, #BMS306) or with the same amounts of their respective solvent (DMSO or H2O ...
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bioRxiv - Cancer Biology 2019Quote: ... Human recombinant bFGF and EGF were purchased from Invitrogen/Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... and 20 ng/ml human recombinant EGF (Thermo Fisher) at 37°C with 5% CO2.
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant IL-2 (2 ng/ml) (Gibco, USA), IL-7 (10 ng/ml ...
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bioRxiv - Cell Biology 2019Quote: ... 20 μg/mL human recombinant insulin (Thermo Fisher Scientific), 20 μg/mL ascorbic acid (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2020Quote: ... and 100 ng/ml human recombinant FGF8 (Gibco, PHG0184). The medium was refreshed daily for 7–10 days as the cells aggregated to form spheroids.
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bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 ng/mL human recombinant FGF (Gibco, #AA10-155), 10 ng/mL heparin sulfate (Stemcell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.01 mg/ml human recombinant insulin (Life Technologies). T-47D cells were cultured in RPMI-1640 Medium (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: Recombinant human Dynactin was expressed using Expi293F cells (Invitrogen) and Expi293 expression medium (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1pM Insulin Human Recombinant Zinc (Life Technologies 12585-014). For generating steatosis cells were supplemented oleic acid-albumin (Sigma-O3008 ...
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bioRxiv - Immunology 2020Quote: ... or 20 ng/ml recombinant human IL4 (Life Technologies), respectively ...
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bioRxiv - Neuroscience 2021Quote: ... MA) and recombinant human insulin was obtained from Gibco.
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bioRxiv - Cancer Biology 2021Quote: ... Human recombinant insulin (10 μg ml−1, Gibco #12585014) was additionally included in the culture media for MCF7 ...
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bioRxiv - Microbiology 2022Quote: ... and 50 IU/mL recombinant human IL-2 (Gibco). IL-2 was also supplemented to each culture for the maintenance of the cells.
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bioRxiv - Neuroscience 2023Quote: ... 20 ng/mL recombinant human EGF (#PHG0313, Gibco, USA), 20 % Knockout Serum Replacement (#10828028 ...
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bioRxiv - Physiology 2023Quote: ... hiPSCs were cultured on recombinant human vitronectin (rhVTN, ThermoFisher) in E8 Flex medium (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... 10 ng/mL recombinant human epidermal growth factor (Invitrogen), 0.1 μM Dibenzazepine (DBZ ...
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bioRxiv - Immunology 2023Quote: ... 294.4 ng/l human recombinant EGF (Gibco, 10450-013) and 300 µM of CaCl2 (Kanto Chemicals ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μg/mL human recombinant insulin (Thermo Fisher Scientific), 1mM sodium pyruvate (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... 50 ng/ml human recombinant EGF (10450-013; Gibco), 3.0% Noggin/R- Spondin-conditioned media ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant human thioredoxin 1 (hTrx) was purchased from ThermoFisher. The plasmid pET-TRSter for heterologous expression of human thioredoxin reductase (hTrxR ...
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bioRxiv - Cancer Biology 2022Quote: ... growth factor 100ug/ml FGF10 (recombinant human) ( Thermo Fisher) and 500nM A-83-01 (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... on recombinant human Vitronectin (A14700, Thermo Fisher / Life Technologies)-coated 6-well plates ...
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bioRxiv - Developmental Biology 2023Quote: ... on recombinant human Vitronectin (A14700, Thermo Fisher / Life Technologies)-coated 6-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg/mL recombinant human insulin (Thermo Fisher Scientific), 0.55 µg/mL human transferrin (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Genomics 2024Quote: ... 20 ng/mL human recombinant EGF (Thermo Fisher PHG0311), and 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2024Quote: ... growth factor 100ug/ml FGF10 (recombinant human) ( Thermo Fisher) and 500nM A-83-01 (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... Affinity separated FLAG-tagged protein was eluted using 35 μL IgG elution buffer (Thermo Fisher), denatured with equal volume of Laemlli buffer ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with FLAG-tagged OGA using Lipofectamine 3000 reagent (Thermo Fisher Scientific) at a ratio 1:2 (μg:μl ...
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bioRxiv - Biochemistry 2024Quote: ... to assess FLAG-tagged protein expression and immunoprecipitation by Immunoblot and silver stain (ThermoFisher Scientific) respectively ...
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bioRxiv - Microbiology 2023Quote: Mono-Fc tagged PvDBP-RII used for SAXS experiments was expressed in Expi293 cells (Thermo Scientific) as described above and purified using the CaptureSelect C-tagXL Affinity Matrix (Thermo Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... and both HRP-conjugated anti-mouse IgG Fc and anti-human IgG Fc were purchased from Invitrogen™ (Waltham ...
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bioRxiv - Microbiology 2021Quote: ... FXa-HRP conjugated anti-human Fc antibody (05-4220, Invitrogen) was used as a detecting antibody ...
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bioRxiv - Immunology 2022Quote: ... or IgG1 Fc (human) protein (Thermo Fisher Scientific, Rockford, IL) was added to a final concentration of 10 mg/mL and samples were incubated with gentle mixing for 2 hours at room temperature ...
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bioRxiv - Immunology 2021Quote: ... then incubated with human Fc receptor coupled to phycoerythrin (ThermoFisher). To set positive gates ...
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bioRxiv - Immunology 2021Quote: ... or specific for the Fc region of human IgG (Invitrogen), the Fc region of human IgM (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... or specific for the Fc region of human IgG (Invitrogen), the Fc region of human IgM (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant hACE2-Fc protein was first biotinylated using EZ-Link Sulfo-NHS-Biotin (ThermoFisher) as the instruction described ...
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bioRxiv - Cell Biology 2020Quote: ... 20 ng/ml recombinant human epidermal growth factor (Invitrogen, USA), 10 ng/ml human basic FGF (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... human recombinant Epidermal Growth Factor (5 ng/ml, ThermoFisher Scientific) and 0.15 mM CaCl₂ (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... coated with 5μg/ml human recombinant vitronectin (Life Technologies, #A14700) in mTesR1 (StemCell Technologies ...