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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Recombinant Human BTN3A1 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and biotinylated proteins were detected using streptavidin-HRP (Invitrogen S911). HRP was detected using enhanced chemiluminescence (ECL ...
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bioRxiv - Biochemistry 2021Quote: ... Biotinylated proteins were isolated using a Streptavidin (Thermo-Fisher Scientific) pull-down assay ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were biotinylated using NHS-PEG4-Biotin (Thermo Scientific, USA) following the manufacturer recommendations ...
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bioRxiv - Microbiology 2023Quote: ... and biotinylated proteins were extracted using Streptavidin beads (Thermo Scientific). Levels of cell surface CFTR in the bead extract were finally analyzed by western blotting ...
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bioRxiv - Biophysics 2023Quote: ... incubated 0.1 mg/mL biotinylated protein A (Thermo Fisher Scientific) in a flow channel for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... Biotinylated proteins were purified using streptavidin-agarose (Pierce/Thermo Fisher) and eluted in 6x SDS-PAGE sample buffer containing 3 mM biotin by boiling at 95 °C for 10 min under reducing conditions ...
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bioRxiv - Neuroscience 2024Quote: ... Biotinylated proteins were purified using Dynabeads MyOne Streptavidin T1 (Invitrogen). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... GS or DA recombinant proteins (970-2527aa) (Thermofisher), GST-AAK1 (1-501aa ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection with purified DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins were coated in PBS buffer (Gibco) at the concentration of 1.2 mg/ml of protein per well ...
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bioRxiv - Immunology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection with purified DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: ... and BMP4 recombinant protein (Gibco; 5 ng/mL). The media was half-changed every day during the differentiation process ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant magnetic protein G beads (Invitrogen, MA, USA) were added for 1 hour at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... HA-tagged polyQ and Myc-tagged polyA proteins were enriched using Pierce™ Anti-HA Agarose (Thermo Fisher Scientific) and Myc-Trap®_A Kit (Chromotek ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Recombinant protein was extracted using BugBuster Protein Extraction Reagent (Thermo Scientific, USA) and purified using cOmplete His-Tag Purification Resin (Roche ...
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bioRxiv - Microbiology 2023Quote: ... containing 1:400 of Human BD Fc Block (ThermoFisher Scientific, BDB564219) per condition ...
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bioRxiv - Immunology 2023Quote: ... along with goat anti-human IgG Fc secondary antibody (polyclonal, ThermoFisher). HLA-ESC was detected with an anti-HLA-E antibody (clone 3D12 ...
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bioRxiv - Neuroscience 2023Quote: ... was recombinantly expressed as an N-terminal Fc-fusion protein (Fc-hTfR1) in Expi293 suspension cells and purified by gravity flow with PierceTM Protein A Agarose (Thermo Fisher Scientific). HFCII probes were loaded with Fc-hTfR1 by thresholding to a 1 nm shift ...
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bioRxiv - Cell Biology 2023Quote: ... 100 pmol (77 nM) of recombinant dynein tail complex or recombinant protein A (ThermoFisher Scientific) were incubated with 100 μL IgG magnetic beads in 1300 μL IgG binding buffer with rotation for 1 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).
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bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... for His-tagged proteins or GST-agarose resin (Thermo Fisher Scientific) for GST-tagged proteins ...
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bioRxiv - Cell Biology 2019Quote: ... HA-tagged proteins were eluted by incubation with HA peptide (ThermoFisher) for 2 × 15 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: To localize GFP-tagged fusion proteins with BODIPY Red (Molecular Probes), transfected cells were grown overnight (16-20h ...
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bioRxiv - Cell Biology 2020Quote: ... To localize GFP-tagged fusion proteins with MitoTracker Red (Molecular Probes), after 16h transfection ...
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bioRxiv - Microbiology 2021Quote: ... for FLAG® (DYKDDDDK)-tagged proteins in iBind Western System (Invitrogen). Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2019Quote: ... tagged proteins were eluted and quantified by BCA (Pierce, ThermoFisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Tagged proteins were visualized using chemiluminescent reagents (Thermo Fisher Scientific 34579).
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bioRxiv - Biophysics 2023Quote: N-terminally 6xHis-tagged human Gβ1 (S2-N340) and human Gγ2 (M1-C68) were synthesized and separately cloned into pFastBac1 (Thermo Fisher) and expressed as described above ...
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bioRxiv - Cancer Biology 2019Quote: ... bait GST-tagged EIF4E or 6xHis-tagged CELF1 proteins (1 μM) were initially bound to glutathione-agarose beads (ThermoFisher Scientific) or Ni2+ beads (GE Healthcare) ...
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg of recombinant 6xHis-4xSUMO2-Strep was biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... B2-Fab was stained with biotinylated goat anti-human IgG (1:1,000, Invitrogen) followed by streptavidin-PE staining ...
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bioRxiv - Microbiology 2020Quote: ... with 0.1 ng/mL human recombinant epidermal growth factor (EGF; Gibco) and 0.05 mg/ml bovine pituitary extract (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... and human recombinant insulin (2.5 µg/mL, Thermo Fisher Scientific, 12585014). From Day 1 to 11 ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length recombinant human LRRK2[G2019S] was purchased from Invitrogen (#A15202). GZD-824 was purchased from Cayman Chemical (#21508 ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2.5 ng/mL of recombinant human IL-2 (Thermofisher, PHC0027). T-cells were co-cultured with the mCherry-Nucleus-7 MCF7 cells in the presence of CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 ng/mL of human recombinant epidermal growth factor (Invitrogen PHG0313), 10 ng/mL of basic fibroblast growth factor (Invitrogen PHG0263 ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 5 ng/mL recombinant human interleukin 2 (Gibco, #PHC0027), 2 mM L-glutamine (Lonza ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 ng/ml recombinant human FGF-Basic (Thermo Fisher Scientific) in a humidified incubator with 5% CO2 at 37°C ...